Method of Reduction Background Fluorescence in Human Fetal Brain Tissue and Quantitative Estimate of the Effect of Photobleaching

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Method of Reduction Background Fluorescence in Human Fetal Brain Tissue and Quantitative Estimate of the Effect of Photobleaching. / Kozubenko, E. A.; Zykin, P. A.; Krasnoshchekova, E. I.; Tkachenko, L. A.; Fedoseeva, K. N.; Kharazova, A. D.

In: Bulletin of Experimental Biology and Medicine, Vol. 171, No. 1, 05.2021, p. 100-104.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{08df1b9268594817a3c3f532d73be085,

title = "Method of Reduction Background Fluorescence in Human Fetal Brain Tissue and Quantitative Estimate of the Effect of Photobleaching",

abstract = "We developed a method of reducing the background fluorescence of samples made from formalin-fixed and paraffin-embedded blocks of the brain of the second-trimester human fetuses. For reducing excess background fluorescence, the samples were subjected to photobleaching using an LED lamp with blue and red emission peaks in the range of visible spectrum in a construction of an original design. The decrease in the background autofluorescence was checked by measuring the intensity of the emitted background fluorescence of the samples and relative abundance of immunopositive structures after immunohistochemical staining. It was found that the proposed method reliably reduced the background fluorescence of the samples, which improved the quality of multicolor immunofluorescence images of the cerebral cortex.",

keywords = "утофлюоресценция, фотовыцветание, фетальный мозг;, слой-специфичные маркеры нейронов, background fluorescence, photobleaching, FETAL BRAIN INJURY, layer-specific markers of neurons",

author = "Kozubenko, {E. A.} and Zykin, {P. A.} and Krasnoshchekova, {E. I.} and Tkachenko, {L. A.} and Fedoseeva, {K. N.} and Kharazova, {A. D.}",

year = "2021",

month = may,

doi = "10.1007/s10517-021-05180-w",

language = "English",

volume = "171",

pages = "100--104",

journal = "Bulletin of Experimental Biology and Medicine",

issn = "0007-4888",

publisher = "Springer Nature",

number = "1",

}

RIS

TY - JOUR

T1 - Method of Reduction Background Fluorescence in Human Fetal Brain Tissue and Quantitative Estimate of the Effect of Photobleaching

AU - Kozubenko, E. A.

AU - Zykin, P. A.

AU - Krasnoshchekova, E. I.

AU - Tkachenko, L. A.

AU - Fedoseeva, K. N.

AU - Kharazova, A. D.

PY - 2021/5

Y1 - 2021/5

N2 - We developed a method of reducing the background fluorescence of samples made from formalin-fixed and paraffin-embedded blocks of the brain of the second-trimester human fetuses. For reducing excess background fluorescence, the samples were subjected to photobleaching using an LED lamp with blue and red emission peaks in the range of visible spectrum in a construction of an original design. The decrease in the background autofluorescence was checked by measuring the intensity of the emitted background fluorescence of the samples and relative abundance of immunopositive structures after immunohistochemical staining. It was found that the proposed method reliably reduced the background fluorescence of the samples, which improved the quality of multicolor immunofluorescence images of the cerebral cortex.

AB - We developed a method of reducing the background fluorescence of samples made from formalin-fixed and paraffin-embedded blocks of the brain of the second-trimester human fetuses. For reducing excess background fluorescence, the samples were subjected to photobleaching using an LED lamp with blue and red emission peaks in the range of visible spectrum in a construction of an original design. The decrease in the background autofluorescence was checked by measuring the intensity of the emitted background fluorescence of the samples and relative abundance of immunopositive structures after immunohistochemical staining. It was found that the proposed method reliably reduced the background fluorescence of the samples, which improved the quality of multicolor immunofluorescence images of the cerebral cortex.

KW - утофлюоресценция

KW - фотовыцветание

KW - фетальный мозг;

KW - слой-специфичные маркеры нейронов

KW - background fluorescence

KW - photobleaching

KW - FETAL BRAIN INJURY

KW - layer-specific markers of neurons

UR - http://www.scopus.com/inward/record.url?scp=85106753748&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/19b1d8a3-6743-3dd0-98f7-fc66a8684e67/

U2 - 10.1007/s10517-021-05180-w

DO - 10.1007/s10517-021-05180-w

M3 - Article

C2 - 34046785

AN - SCOPUS:85106753748

VL - 171

SP - 100

EP - 104

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

SN - 0007-4888

IS - 1

ER -

ID: 74585574