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Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria. / Vasileva, A.; Skopina, M.; Averina, S.; Gavrilova, O.; Ivanikova, N.; Pinevich, A.

In: Journal of Great Lakes Research, Vol. 43, 2017, p. 43-54.

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Vasileva, A. ; Skopina, M. ; Averina, S. ; Gavrilova, O. ; Ivanikova, N. ; Pinevich, A. / Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria. In: Journal of Great Lakes Research. 2017 ; Vol. 43. pp. 43-54.

BibTeX

@article{22e91fea37824318bab9b850dc78d312,
title = "Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria",
abstract = "{\textcopyright} 2017.Despite Synechococcus picocyanobacteria being important in lacustrine ecosystems, they have received less attention than their marine counterparts. We describe for the first time, using 454 pyrosequencing, the diversity of Lake Onego cyanobacteria. The majority (97.52%) of 16S rRNA gene sequences observed in Synechococcus collected from the epilimnion belonged to OTUs (operational taxonomic units) I-XVI. The obtained pyrotags were classified, together with specially created database of 770 reference sequences, via the M-pick algorithm and compared with traditional (cited in the literature) assemblages, which for reason of reliability were reappraised. The entire set of sequences were attributed to freshwater Synechococcus phylotypes (FSP) 1-45. Most of the Lake Onego Synechococcus sequences (47.47%) belonged to OTU VII within FSP 14, which comprised ubiquitous strains, whereas the low-abundance FSP 20 (5% of sequences) demonstrated a limited geographical distribution. The study of connection between La",
author = "A. Vasileva and M. Skopina and S. Averina and O. Gavrilova and N. Ivanikova and A. Pinevich",
year = "2017",
doi = "10.1016/j.jglr.2017.03.003",
language = "English",
volume = "43",
pages = "43--54",
journal = "Journal of Great Lakes Research",
issn = "0380-1330",
publisher = "International Association of Great Lakes Research",

}

RIS

TY - JOUR

T1 - Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria

AU - Vasileva, A.

AU - Skopina, M.

AU - Averina, S.

AU - Gavrilova, O.

AU - Ivanikova, N.

AU - Pinevich, A.

PY - 2017

Y1 - 2017

N2 - © 2017.Despite Synechococcus picocyanobacteria being important in lacustrine ecosystems, they have received less attention than their marine counterparts. We describe for the first time, using 454 pyrosequencing, the diversity of Lake Onego cyanobacteria. The majority (97.52%) of 16S rRNA gene sequences observed in Synechococcus collected from the epilimnion belonged to OTUs (operational taxonomic units) I-XVI. The obtained pyrotags were classified, together with specially created database of 770 reference sequences, via the M-pick algorithm and compared with traditional (cited in the literature) assemblages, which for reason of reliability were reappraised. The entire set of sequences were attributed to freshwater Synechococcus phylotypes (FSP) 1-45. Most of the Lake Onego Synechococcus sequences (47.47%) belonged to OTU VII within FSP 14, which comprised ubiquitous strains, whereas the low-abundance FSP 20 (5% of sequences) demonstrated a limited geographical distribution. The study of connection between La

AB - © 2017.Despite Synechococcus picocyanobacteria being important in lacustrine ecosystems, they have received less attention than their marine counterparts. We describe for the first time, using 454 pyrosequencing, the diversity of Lake Onego cyanobacteria. The majority (97.52%) of 16S rRNA gene sequences observed in Synechococcus collected from the epilimnion belonged to OTUs (operational taxonomic units) I-XVI. The obtained pyrotags were classified, together with specially created database of 770 reference sequences, via the M-pick algorithm and compared with traditional (cited in the literature) assemblages, which for reason of reliability were reappraised. The entire set of sequences were attributed to freshwater Synechococcus phylotypes (FSP) 1-45. Most of the Lake Onego Synechococcus sequences (47.47%) belonged to OTU VII within FSP 14, which comprised ubiquitous strains, whereas the low-abundance FSP 20 (5% of sequences) demonstrated a limited geographical distribution. The study of connection between La

U2 - 10.1016/j.jglr.2017.03.003

DO - 10.1016/j.jglr.2017.03.003

M3 - Article

VL - 43

SP - 43

EP - 54

JO - Journal of Great Lakes Research

JF - Journal of Great Lakes Research

SN - 0380-1330

ER -

ID: 7924648