Research output: Contribution to journal › Article › peer-review
Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria. / Vasileva, A.; Skopina, M.; Averina, S.; Gavrilova, O.; Ivanikova, N.; Pinevich, A.
In: Journal of Great Lakes Research, Vol. 43, 2017, p. 43-54.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Metagenomic analysis in Lake Onego (Russia) Synechococcus cyanobacteria
AU - Vasileva, A.
AU - Skopina, M.
AU - Averina, S.
AU - Gavrilova, O.
AU - Ivanikova, N.
AU - Pinevich, A.
PY - 2017
Y1 - 2017
N2 - © 2017.Despite Synechococcus picocyanobacteria being important in lacustrine ecosystems, they have received less attention than their marine counterparts. We describe for the first time, using 454 pyrosequencing, the diversity of Lake Onego cyanobacteria. The majority (97.52%) of 16S rRNA gene sequences observed in Synechococcus collected from the epilimnion belonged to OTUs (operational taxonomic units) I-XVI. The obtained pyrotags were classified, together with specially created database of 770 reference sequences, via the M-pick algorithm and compared with traditional (cited in the literature) assemblages, which for reason of reliability were reappraised. The entire set of sequences were attributed to freshwater Synechococcus phylotypes (FSP) 1-45. Most of the Lake Onego Synechococcus sequences (47.47%) belonged to OTU VII within FSP 14, which comprised ubiquitous strains, whereas the low-abundance FSP 20 (5% of sequences) demonstrated a limited geographical distribution. The study of connection between La
AB - © 2017.Despite Synechococcus picocyanobacteria being important in lacustrine ecosystems, they have received less attention than their marine counterparts. We describe for the first time, using 454 pyrosequencing, the diversity of Lake Onego cyanobacteria. The majority (97.52%) of 16S rRNA gene sequences observed in Synechococcus collected from the epilimnion belonged to OTUs (operational taxonomic units) I-XVI. The obtained pyrotags were classified, together with specially created database of 770 reference sequences, via the M-pick algorithm and compared with traditional (cited in the literature) assemblages, which for reason of reliability were reappraised. The entire set of sequences were attributed to freshwater Synechococcus phylotypes (FSP) 1-45. Most of the Lake Onego Synechococcus sequences (47.47%) belonged to OTU VII within FSP 14, which comprised ubiquitous strains, whereas the low-abundance FSP 20 (5% of sequences) demonstrated a limited geographical distribution. The study of connection between La
U2 - 10.1016/j.jglr.2017.03.003
DO - 10.1016/j.jglr.2017.03.003
M3 - Article
VL - 43
SP - 43
EP - 54
JO - Journal of Great Lakes Research
JF - Journal of Great Lakes Research
SN - 0380-1330
ER -
ID: 7924648