Research output: Contribution to journal › Article › peer-review
Metabolic Profiling of Wheat Seedlings Under Oxygen Deficiency and Subsequent Reaeration Conditions. / Yemelyanov, V.V.; Puzanskiy, R.K.; Bogdanova, E.M.; Vanisov, S.A.; Dubrovskiy, M.D.; Lastochkin, V.V.; Kirpichnikova, A.A.; Brykova, A.N.; Shavarda, A.L.; Shishova, M.F.
In: International Journal of Molecular Sciences, Vol. 26, No. 23, 2025.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Metabolic Profiling of Wheat Seedlings Under Oxygen Deficiency and Subsequent Reaeration Conditions
AU - Yemelyanov, V.V.
AU - Puzanskiy, R.K.
AU - Bogdanova, E.M.
AU - Vanisov, S.A.
AU - Dubrovskiy, M.D.
AU - Lastochkin, V.V.
AU - Kirpichnikova, A.A.
AU - Brykova, A.N.
AU - Shavarda, A.L.
AU - Shishova, M.F.
N1 - Export Date: 29 January 2026; Cited By: 0
PY - 2025
Y1 - 2025
N2 - The ability of plants to survive oxygen deficiency is associated with significant changes in metabolism. Metabolic profiling of wheat seedlings under anoxia and subsequent reoxygenation conditions was performed using GC-MS. A total of 374 and 298 compounds were detected in root and shoot metabolomes, respectively. All intermediates of central metabolism were identified. Early anoxic responses of root and shoot metabolomes showed similarity, leading to the accumulation of amino acids (Ala, GABA and Tyr), carboxylates (lactate and succinate), nucleotides and amines, together with a decrease in sugars. The metabolic response to long-term anoxia varied significantly in the roots and shoots of wheat seedlings and was related to the redistribution of carbon flux from glycolysis predominantly to lipids in the roots, while it was directed to carboxylates and GABA in the shoots. Imposition of 24 h of reaeration after short-term anoxia (6 h) switched the metabolome toward a normoxic profile, predominantly in roots. Anaerobically down-regulated metabolites were accumulated, while anaerobic intermediates were depleted post-anoxia. The effects of more prolonged anoxia on wheat seedling metabolomes were less reversible, particularly in shoots. Interestingly, several metabolites with not fully understood roles (e.g., hydroxyl carboxylates, α,ω-dicarboxylic acids, polyols) were detected under anoxic conditions in wheat seedlings, which could potentially serve as markers of plant sensitivity to oxygen deficiency. © 2025 by the authors.
AB - The ability of plants to survive oxygen deficiency is associated with significant changes in metabolism. Metabolic profiling of wheat seedlings under anoxia and subsequent reoxygenation conditions was performed using GC-MS. A total of 374 and 298 compounds were detected in root and shoot metabolomes, respectively. All intermediates of central metabolism were identified. Early anoxic responses of root and shoot metabolomes showed similarity, leading to the accumulation of amino acids (Ala, GABA and Tyr), carboxylates (lactate and succinate), nucleotides and amines, together with a decrease in sugars. The metabolic response to long-term anoxia varied significantly in the roots and shoots of wheat seedlings and was related to the redistribution of carbon flux from glycolysis predominantly to lipids in the roots, while it was directed to carboxylates and GABA in the shoots. Imposition of 24 h of reaeration after short-term anoxia (6 h) switched the metabolome toward a normoxic profile, predominantly in roots. Anaerobically down-regulated metabolites were accumulated, while anaerobic intermediates were depleted post-anoxia. The effects of more prolonged anoxia on wheat seedling metabolomes were less reversible, particularly in shoots. Interestingly, several metabolites with not fully understood roles (e.g., hydroxyl carboxylates, α,ω-dicarboxylic acids, polyols) were detected under anoxic conditions in wheat seedlings, which could potentially serve as markers of plant sensitivity to oxygen deficiency. © 2025 by the authors.
KW - adaptation
KW - anoxia
KW - hydroxyl carboxylic acids
KW - metabolomics
KW - reoxygenation
KW - tolerance
KW - Triticum aestivum
KW - 1,3 propanediamine
KW - 2 hydroxyglutaric acid
KW - 2,3 butanediol
KW - 3 hydroxybutyric acid
KW - 4 hydroxybutyric acid
KW - acid
KW - aconitic acid
KW - acylglycerol
KW - adenine
KW - adenosine
KW - allantoin
KW - ascorbic acid
KW - butyric acid
KW - carbohydrate
KW - carboxylic acid
KW - dicarboxylic acid
KW - fatty acid
KW - fatty acid derivative
KW - fructose 6 phosphate
KW - fumaric acid
KW - gluconic acid
KW - glucose 6 phosphate
KW - glyceric acid
KW - glycerol
KW - glyoxylic acid
KW - guanine
KW - hexose
KW - hydracrylic acid
KW - inositol
KW - inositol phosphate
KW - lactic acid
KW - malic acid
KW - nitrogen
KW - nucleotide
KW - oligosaccharide
KW - ornithine
KW - oxygen
KW - pentose
KW - polyol
KW - propionic acid
KW - pyruvic acid
KW - shikimic acid
KW - starch
KW - succinic acid
KW - sucrose
KW - terpenoid
KW - thymine
KW - uracil
KW - urate
KW - uridine
KW - xanthine
KW - aeration
KW - Article
KW - citric acid cycle
KW - comparative study
KW - controlled study
KW - dynamics
KW - hypoxia
KW - metabolic fingerprinting
KW - metabolite set enrichment analysis
KW - metabolome
KW - nucleotide metabolism
KW - plant root
KW - seedling
KW - shoot
KW - steroid metabolism
KW - wheat
KW - mass fragmentography
KW - metabolism
KW - procedures
KW - Gas Chromatography-Mass Spectrometry
KW - Metabolome
KW - Metabolomics
KW - Oxygen
KW - Plant Roots
KW - Plant Shoots
KW - Seedlings
KW - Triticum
U2 - 10.3390/ijms262311610
DO - 10.3390/ijms262311610
M3 - статья
VL - 26
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 23
ER -
ID: 147995724