TY - JOUR

T1 - MESENCHYMAL STEM CELLS IN COMPLEX TREATMENT OF URINARY TRACT DISEASES: A LITERATURE REVIEW AND OUR OWN EXPERIENCE

AU - Орлова, Н.В.

AU - Муравьев , Александр Николаевич

AU - Горелова, Анна Андреевна

AU - Ремезова, Анна Николаевна

AU - Виноградова , Татьяна Ивановна

AU - Юдинцева , Наталия Михайловна

AU - Нащекина, Юлия Александровна

AU - Горбунов, Александр Игоревич

AU - ЛЕБЕДЕВ, АЛЕКСАНДР АНАТОЛЬЕВИЧ

AU - Горелов, Андрей Игоревич

AU - Яблонский, Петр Казимирович

PY - 2023/3/30

Y1 - 2023/3/30

N2 - Over recent years, the use of mesenchymal stem cells (MSCs) has been increasingly studied in complex therapy of various diseases, as well as in reconstruction of damaged organs and tissues. In particular, immunoregulatory properties of MSCs are in scope, manifesting both by a direct effect on the target cells, and via signaling molecules and cytokines. This review is devoted to perspectives of using MSCs in urinary tract disorders, including the description of immunosuppressive mechanisms and the reasons for using this cell type in experimental and clinical practice. We also present our own experience of bladder reconstruction in animal model. We are currently studying the substitution cystoplasty using tissue-engineered constructs. The study is based on data from our previous experimental studies. We are testing poly-l-lactide/silk fibroin scaffold of different sizes seeded with MSCs using a rabbit bladder resection model, in order to find out what part of the bladder we could substitute. At this stage, there are positive results of 5-to 10-mL substitutional cystoplasty. According to multislice computed tomography of the abdominal cavity and small pelvis (native study and with intravesical administration of a radiopaque agent) 4 weeks post surgery, a bladder of physiological capacity (10-11 ml) is determined, without streaks of a contrast agent. Also, 4 weeks after the operation, the anastomosis zone is macroscopically consistent, the tissue-engineered structure is determined at the implantation site. Conclusion The unique immunomodulatory properties of mesenchymal stem cells, combined with their multipotency, allow their usage in complex treatment of different clinical disorders. Moreover, both systemic use of MSCs and their application for tissue engineering constructs are feasible. The usage of such constructs consisting of a composite matrix and rabbit mesenchymal stem cells, proved to be effective for reconstruction of small bladder defects. Further advances in development of the multicomponent grafts with allogeneic cells may improve the treatment outcomes in distinct disorders where autologous biomaterial is not available.

AB - Over recent years, the use of mesenchymal stem cells (MSCs) has been increasingly studied in complex therapy of various diseases, as well as in reconstruction of damaged organs and tissues. In particular, immunoregulatory properties of MSCs are in scope, manifesting both by a direct effect on the target cells, and via signaling molecules and cytokines. This review is devoted to perspectives of using MSCs in urinary tract disorders, including the description of immunosuppressive mechanisms and the reasons for using this cell type in experimental and clinical practice. We also present our own experience of bladder reconstruction in animal model. We are currently studying the substitution cystoplasty using tissue-engineered constructs. The study is based on data from our previous experimental studies. We are testing poly-l-lactide/silk fibroin scaffold of different sizes seeded with MSCs using a rabbit bladder resection model, in order to find out what part of the bladder we could substitute. At this stage, there are positive results of 5-to 10-mL substitutional cystoplasty. According to multislice computed tomography of the abdominal cavity and small pelvis (native study and with intravesical administration of a radiopaque agent) 4 weeks post surgery, a bladder of physiological capacity (10-11 ml) is determined, without streaks of a contrast agent. Also, 4 weeks after the operation, the anastomosis zone is macroscopically consistent, the tissue-engineered structure is determined at the implantation site. Conclusion The unique immunomodulatory properties of mesenchymal stem cells, combined with their multipotency, allow their usage in complex treatment of different clinical disorders. Moreover, both systemic use of MSCs and their application for tissue engineering constructs are feasible. The usage of such constructs consisting of a composite matrix and rabbit mesenchymal stem cells, proved to be effective for reconstruction of small bladder defects. Further advances in development of the multicomponent grafts with allogeneic cells may improve the treatment outcomes in distinct disorders where autologous biomaterial is not available.

KW - Mesenchymal stem cells

KW - effects of mesenchymal stem cells

KW - small bladder

KW - tissue-engineering construction

UR - https://elibrary.ru/item.asp?id=50505272

UR - https://www.mendeley.com/catalogue/e3e019bd-af5c-3d4c-bfd0-15fb51876f96/

U2 - 10.18620/ctt-1866-8836-2023-12-1-51-59

DO - 10.18620/ctt-1866-8836-2023-12-1-51-59

M3 - Article

VL - 12

SP - 51

EP - 59

JO - Cellular Therapy and Transplantation

JF - Cellular Therapy and Transplantation

SN - 1867-416X

IS - 1

ER -