Research output: Contribution to journal › Article › peer-review
Mechanisms underlying iron and zinc transport to axis organs in grain during early seedling development of maize. / Bityutskii, Nikolai P.; Davydovskaya, Elena N.; Malyuga, Ekaterina A.; Yakkonen, Kyrill L.
In: Journal of Plant Nutrition, Vol. 27, No. 9, 2004, p. 1525-1541.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Mechanisms underlying iron and zinc transport to axis organs in grain during early seedling development of maize
AU - Bityutskii, Nikolai P.
AU - Davydovskaya, Elena N.
AU - Malyuga, Ekaterina A.
AU - Yakkonen, Kyrill L.
N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004
Y1 - 2004
N2 - The role of both acidity release and ferric reduction by scutellum was investigated in relation to iron (Fe) mobilization from endosperm and transport to axis organs (shoot + root) during early seedling development of maize (Zea mays L.). Effect of scutellum zinc (Zn) transfer to growing axis organs was also evaluated. Acidity release and translocation of Fe or Zn from an agar medium to axis organs were decreased when scutellum dorsal sides of intact seedlings were embedded into a pH buffered agar medium. Across genotypes root growth during at least 3 days was correlated to scutellar acidity release. Orthovanadate or pyrazole (1 mM) did not inhibit Fe translocation to axis organs. Thus, Fe translocation was not closely associated with scutellum plasma membrane H +-ATPases or alcohol dehydrogenase. Addition of Fe 2+-citrate facilitated Fe translocation from agar medium to axis organs by 20%, compared to FeSO4. Extracellular reduction of Fe 2+ to Fe3+ was associated with release of reductants from scutellum, which was dramatically inhibited by low temperature (7°C). This process was not affected by solution pH within range of 3.5-6.5 units or by copper (Cu2+), Zn2+, and manganese (Mn2+) added to assay-solution Fe.
AB - The role of both acidity release and ferric reduction by scutellum was investigated in relation to iron (Fe) mobilization from endosperm and transport to axis organs (shoot + root) during early seedling development of maize (Zea mays L.). Effect of scutellum zinc (Zn) transfer to growing axis organs was also evaluated. Acidity release and translocation of Fe or Zn from an agar medium to axis organs were decreased when scutellum dorsal sides of intact seedlings were embedded into a pH buffered agar medium. Across genotypes root growth during at least 3 days was correlated to scutellar acidity release. Orthovanadate or pyrazole (1 mM) did not inhibit Fe translocation to axis organs. Thus, Fe translocation was not closely associated with scutellum plasma membrane H +-ATPases or alcohol dehydrogenase. Addition of Fe 2+-citrate facilitated Fe translocation from agar medium to axis organs by 20%, compared to FeSO4. Extracellular reduction of Fe 2+ to Fe3+ was associated with release of reductants from scutellum, which was dramatically inhibited by low temperature (7°C). This process was not affected by solution pH within range of 3.5-6.5 units or by copper (Cu2+), Zn2+, and manganese (Mn2+) added to assay-solution Fe.
KW - Axis organs
KW - Grain
KW - Iron
KW - Maize
KW - Transport
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=4444258512&partnerID=8YFLogxK
U2 - 10.1081/PLN-200025996
DO - 10.1081/PLN-200025996
M3 - Article
VL - 27
SP - 1525
EP - 1541
JO - Journal of Plant Nutrition
JF - Journal of Plant Nutrition
SN - 0190-4167
IS - 9
ER -
ID: 5025950