TY - JOUR

T1 - Major chromosome rearrangements in intergeneric wheat × rye hybrids in compatible and incompatible crosses detected by GBS read coverage analysis

AU - Tikhenko, Natalia

AU - Haupt, Max

AU - Fuchs, Jörg

AU - Perovic, Dragan

AU - Himmelbach, Axel

AU - Mascher, Martin

AU - Houben, Andreas

AU - Rutten, Twan

AU - Nagel, Manuela

AU - Tsvetkova, Natalia V

AU - Sehmisch, Stefanie

AU - Börner, Andreas

N1 - © 2024. The Author(s).

PY - 2024/5/14

Y1 - 2024/5/14

N2 - The presence of incompatibility alleles in primary amphidiploids constitutes a reproductive barrier in newly synthesized wheat-rye hybrids. To overcome this barrier, the genome stabilization process includes large-scale chromosome rearrangements. In incompatible crosses resulting in fertile amphidiploids, the elimination of one of the incompatible alleles Eml-A1 or Eml-R1b can occur already in the somatic tissue of the wheat × rye hybrid embryo. We observed that the interaction of incompatible loci Eml-A1 of wheat and Eml-R1b of rye after overcoming embryo lethality leads to hybrid sterility in primary triticale. During subsequent seed reproductions (R1, R2 or R3) most of the chromosomes of A, B, D and R subgenomes undergo rearrangement or eliminations to increase the fertility of the amphidiploid by natural selection. Genotyping-by-sequencing (GBS) coverage analysis showed that improved fertility is associated with the elimination of entire and partial chromosomes carrying factors that either cause the disruption of plant development in hybrid plants or lead to the restoration of the euploid number of chromosomes (2n = 56) in the absence of one of the incompatible alleles. Highly fertile offspring obtained in compatible and incompatible crosses can be successfully adapted for the production of triticale pre-breeding stocks.

AB - The presence of incompatibility alleles in primary amphidiploids constitutes a reproductive barrier in newly synthesized wheat-rye hybrids. To overcome this barrier, the genome stabilization process includes large-scale chromosome rearrangements. In incompatible crosses resulting in fertile amphidiploids, the elimination of one of the incompatible alleles Eml-A1 or Eml-R1b can occur already in the somatic tissue of the wheat × rye hybrid embryo. We observed that the interaction of incompatible loci Eml-A1 of wheat and Eml-R1b of rye after overcoming embryo lethality leads to hybrid sterility in primary triticale. During subsequent seed reproductions (R1, R2 or R3) most of the chromosomes of A, B, D and R subgenomes undergo rearrangement or eliminations to increase the fertility of the amphidiploid by natural selection. Genotyping-by-sequencing (GBS) coverage analysis showed that improved fertility is associated with the elimination of entire and partial chromosomes carrying factors that either cause the disruption of plant development in hybrid plants or lead to the restoration of the euploid number of chromosomes (2n = 56) in the absence of one of the incompatible alleles. Highly fertile offspring obtained in compatible and incompatible crosses can be successfully adapted for the production of triticale pre-breeding stocks.

KW - Triticum/genetics

KW - Secale/genetics

KW - Chromosomes, Plant/genetics

KW - Hybridization, Genetic

KW - Crosses, Genetic

KW - Alleles

KW - Genotyping Techniques

KW - Hybrid sterility

KW - Coverage analysis

KW - Chromosome rearrangements

KW - Embryo lethality

KW - Incompatible crosses

KW - Genotyping-by-sequencing GBS

KW - Wheat-rye amphidiploids

UR - https://www.mendeley.com/catalogue/764d4a55-36fa-3ea7-9fc8-cf0a24000e87/

U2 - 10.1038/s41598-024-61622-1

DO - 10.1038/s41598-024-61622-1

M3 - Article

C2 - 38745019

VL - 14

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 11010

ER -