TY - JOUR

T1 - Magnetron plasma mediated immobilization of hyaluronic acid for the development of functional double-sided biodegradable vascular graft

AU - Kudryavtseva, Valeriya

AU - Stankevich, Ksenia

AU - Kozelskaya, Anna

AU - Kibler, Elina

AU - Zhukov, Yuri

AU - Malashicheva, Anna

AU - Golovkin, Alexey

AU - Mishanin, Alexander

AU - Filimonov, Victor

AU - Bolbasov, Evgeny

AU - Tverdokhlebov, Sergei

N1 - Funding Information: This study was financially supported by the Ministry of Science and Higher Education of the Russian Federation (State Project “Science” № FSWW-2020-0011). The authors acknowledge the Resource Centre of Saint-Petersburg State University “Physical methods of surface investigation” for conducting XPS study. Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/1

Y1 - 2020/11/1

N2 - The clinical need for vascular grafts is associated with cardiovascular diseases frequently leading to fatal outcomes. Artificial vessels based on bioresorbable polymers can replace the damaged vascular tissue or create a bypass path for blood flow while stimulating regeneration of a blood vessel in situ. However, the problem of proper conditions for the cells to grow on the vascular graft from the adventitia while maintaining its mechanical integrity of the luminal surface remains a challenge. In this work, we propose a two-stage technology for processing electrospun vascular graft from polycaprolactone, which consists of plasma treatment and subsequent immobilization of hyaluronic acid on its surface producing thin double-sided graft with one hydrophilic and one hydrophobic side. Plasma modification activates the polymer surfaces and produces a thin layer for linker-free immobilisation of bioactive molecules, thereby producing materials with unique properties. Proposed modification does not affect the morphology or mechanical properties of the graft and improves cell adhesion. The proposed approach can potentially be used for various biodegradable polymers such as polylactic acid, polyglycolide and their copolymers and blends, with a hydrophilic inner surface and a hydrophobic outer surface.

AB - The clinical need for vascular grafts is associated with cardiovascular diseases frequently leading to fatal outcomes. Artificial vessels based on bioresorbable polymers can replace the damaged vascular tissue or create a bypass path for blood flow while stimulating regeneration of a blood vessel in situ. However, the problem of proper conditions for the cells to grow on the vascular graft from the adventitia while maintaining its mechanical integrity of the luminal surface remains a challenge. In this work, we propose a two-stage technology for processing electrospun vascular graft from polycaprolactone, which consists of plasma treatment and subsequent immobilization of hyaluronic acid on its surface producing thin double-sided graft with one hydrophilic and one hydrophobic side. Plasma modification activates the polymer surfaces and produces a thin layer for linker-free immobilisation of bioactive molecules, thereby producing materials with unique properties. Proposed modification does not affect the morphology or mechanical properties of the graft and improves cell adhesion. The proposed approach can potentially be used for various biodegradable polymers such as polylactic acid, polyglycolide and their copolymers and blends, with a hydrophilic inner surface and a hydrophobic outer surface.

KW - Electrospinning

KW - Hyaluronic acid

KW - Plasma

KW - Polycaprolactone

KW - Superhydrophilicity

KW - Vascular graft

KW - PERFORMANCE

KW - ADSORPTION

KW - STENT

KW - FILMS

KW - SURFACE

KW - XPS

KW - SCAFFOLDS

UR - http://www.scopus.com/inward/record.url?scp=85087959750&partnerID=8YFLogxK

U2 - 10.1016/j.apsusc.2020.147196

DO - 10.1016/j.apsusc.2020.147196

M3 - Article

AN - SCOPUS:85087959750

VL - 529

JO - Applied Surface Science

JF - Applied Surface Science

SN - 0169-4332

M1 - 147196

ER -