LINE-related component of mouse heterochromatin and complex chromocenters' composition

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LINE-related component of mouse heterochromatin and complex chromocenters' composition. / Kuznetsova, I. S.; Ostromyshenskii, D. I.; Komissarov, A. S.; Prusov, A. N.; Waisertreiger, I. S.; Gorbunova, A. V.; Trifonov, V. A.; Ferguson-Smith, M. A.; Podgornaya, O. I.

In: Chromosome Research, Vol. 24, No. 3, 2016, p. 309-323.

Research output: Contribution to journal › Article

Author

Kuznetsova, I. S. ; Ostromyshenskii, D. I. ; Komissarov, A. S. ; Prusov, A. N. ; Waisertreiger, I. S. ; Gorbunova, A. V. ; Trifonov, V. A. ; Ferguson-Smith, M. A. ; Podgornaya, O. I. / LINE-related component of mouse heterochromatin and complex chromocenters' composition. In: Chromosome Research. 2016 ; Vol. 24, No. 3. pp. 309-323.

BibTeX

@article{da392e0806d540e0ad0cf4a3021b5bbd,

title = "LINE-related component of mouse heterochromatin and complex chromocenters' composition",

abstract = "Chromocenters are interphase nuclear landmark structures of constitutive heterochromatin. The tandem repeat (TR)-enriched parts of different chromosomes cluster together in chromocenters. There has been progress in recent years in determining the protein content of chromocenters, although it is not clear which DNA sequences underly constitutive heterochromatin apart from the TRs. The aim of the current work was to find out which DNA sequences besides TRs are involved in chromocenters' formation. Biochemically isolated chromocenters and microdissected centromeric regions were amplified by DOP-PCR, then cloned and sequenced. Alignment to Repbase, the mouse reference genome and WGS databases separated the sequences from both libraries into three groups: (1) sequences with similarity to pericentromere mouse major satellite; (2) sequences without similarity to any repetitive sequences; (3) sequences with similarity to long interspersed nuclear elements (LINEs). LINE-related sequences have a disperse pattern distri",

keywords = "Bioinformatics analysis, Fluorescent in situ hybridization (FISH), Heterochromatin, LINE, Mouse genome, Tandem repeat",

author = "Kuznetsova, {I. S.} and Ostromyshenskii, {D. I.} and Komissarov, {A. S.} and Prusov, {A. N.} and Waisertreiger, {I. S.} and Gorbunova, {A. V.} and Trifonov, {V. A.} and Ferguson-Smith, {M. A.} and Podgornaya, {O. I.}",

year = "2016",

doi = "10.1007/s10577-016-9525-9",

language = "English",

volume = "24",

pages = "309--323",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Nature",

number = "3",

}

RIS

TY - JOUR

T1 - LINE-related component of mouse heterochromatin and complex chromocenters' composition

AU - Kuznetsova, I. S.

AU - Ostromyshenskii, D. I.

AU - Komissarov, A. S.

AU - Prusov, A. N.

AU - Waisertreiger, I. S.

AU - Gorbunova, A. V.

AU - Trifonov, V. A.

AU - Ferguson-Smith, M. A.

AU - Podgornaya, O. I.

PY - 2016

Y1 - 2016

N2 - Chromocenters are interphase nuclear landmark structures of constitutive heterochromatin. The tandem repeat (TR)-enriched parts of different chromosomes cluster together in chromocenters. There has been progress in recent years in determining the protein content of chromocenters, although it is not clear which DNA sequences underly constitutive heterochromatin apart from the TRs. The aim of the current work was to find out which DNA sequences besides TRs are involved in chromocenters' formation. Biochemically isolated chromocenters and microdissected centromeric regions were amplified by DOP-PCR, then cloned and sequenced. Alignment to Repbase, the mouse reference genome and WGS databases separated the sequences from both libraries into three groups: (1) sequences with similarity to pericentromere mouse major satellite; (2) sequences without similarity to any repetitive sequences; (3) sequences with similarity to long interspersed nuclear elements (LINEs). LINE-related sequences have a disperse pattern distri

AB - Chromocenters are interphase nuclear landmark structures of constitutive heterochromatin. The tandem repeat (TR)-enriched parts of different chromosomes cluster together in chromocenters. There has been progress in recent years in determining the protein content of chromocenters, although it is not clear which DNA sequences underly constitutive heterochromatin apart from the TRs. The aim of the current work was to find out which DNA sequences besides TRs are involved in chromocenters' formation. Biochemically isolated chromocenters and microdissected centromeric regions were amplified by DOP-PCR, then cloned and sequenced. Alignment to Repbase, the mouse reference genome and WGS databases separated the sequences from both libraries into three groups: (1) sequences with similarity to pericentromere mouse major satellite; (2) sequences without similarity to any repetitive sequences; (3) sequences with similarity to long interspersed nuclear elements (LINEs). LINE-related sequences have a disperse pattern distri

KW - Bioinformatics analysis

KW - Fluorescent in situ hybridization (FISH)

KW - Heterochromatin

KW - LINE

KW - Mouse genome

KW - Tandem repeat

U2 - 10.1007/s10577-016-9525-9

DO - 10.1007/s10577-016-9525-9

M3 - Article

VL - 24

SP - 309

EP - 323

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 3

ER -

ID: 7630646