In a great number of primate species bipedal stance was defined as a factor increasing manual laterality for reaching. For deeper understanding of the reasons causing this phenomenon it is important to investigate manual preferences in non-primate mammalian species with bipedal locomotion as a preferred gait, e.g., bipedal hopping marsupials. We studied laterality in forelimb use in red-necked wallabies during their usual daily activity in five different zoos. During feeding from the bipedal position adult red-necked wallabies (N = 27) displayed group-level left forelimb preference with the great majority of individuals being lateralized. However, no lateralization has been found on the group-level during feeding from the quadrupedal position, with only few animals expressing individual preferences. Wallabies showed significant group-level bias to use the right forelimb for supporting the body in the tripedal stance with nearly half individuals being lateralized. On a smaller sample (N = 9) a group-level tend