Research output: Contribution to journal › Article › peer-review
Light scatter by cell suspensions in normal conditions and on exposure to external agents. / Ostashevskii, I. Ya; Sungurov, A. Yu; Volchkov, V. A.
In: Biophysics, Vol. 20, No. 5, 1975, p. 868-873.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Light scatter by cell suspensions in normal conditions and on exposure to external agents
AU - Ostashevskii, I. Ya
AU - Sungurov, A. Yu
AU - Volchkov, V. A.
PY - 1975
Y1 - 1975
N2 - The authors have studied the characteristics of the scatter of light by cell suspensions in norpemal conditions (pH 7·2; 20 °C) and on exposure to external agents (change in pH, rise in temrature). It is shown that in normal conditions for large cells (radius 3-7 μ) the turbidity weakly depends on 1/λ; power n = 0·2-0·3. On damage to the cells the values of n rises and the course of the dependence coincides with the character of the increase in the fraction of damaged cells determined from the eosin test. The changes in the parameters of the total light scatter on damage to the cells are related to the increase in the contribution of scatter by the intracellular microstructure.
AB - The authors have studied the characteristics of the scatter of light by cell suspensions in norpemal conditions (pH 7·2; 20 °C) and on exposure to external agents (change in pH, rise in temrature). It is shown that in normal conditions for large cells (radius 3-7 μ) the turbidity weakly depends on 1/λ; power n = 0·2-0·3. On damage to the cells the values of n rises and the course of the dependence coincides with the character of the increase in the fraction of damaged cells determined from the eosin test. The changes in the parameters of the total light scatter on damage to the cells are related to the increase in the contribution of scatter by the intracellular microstructure.
UR - http://www.scopus.com/inward/record.url?scp=0016619555&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0016619555
VL - 20
SP - 868
EP - 873
JO - Biophysics (Russian Federation)
JF - Biophysics (Russian Federation)
SN - 0006-3509
IS - 5
ER -
ID: 85034709