Research output: Contribution to journal › Article › peer-review
Lifetime oxygen sensors based on block copolymer micelles and non-covalent human serum albumin adducts bearing phosphorescent near-infrared iridium(III) complex. / Elistratova, Anastasiia A.; Kritchenkov, Ilya S.; Lezov, Alexey A.; Gubarev, Alexander S.; Solomatina, Anastasia I.; Kachkin, Daniel V.; Shcherbina, Nadezhda A.; Liao, Yu Chan; Liu, Yi Chun; Yang, Ya Yun; Tsvetkov, Nikolai V.; Chelushkin, Pavel S.; Chou, Pi Tai; Tunik, Sergey P.
In: European Polymer Journal, Vol. 159, 110761, 05.10.2021.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Lifetime oxygen sensors based on block copolymer micelles and non-covalent human serum albumin adducts bearing phosphorescent near-infrared iridium(III) complex
AU - Elistratova, Anastasiia A.
AU - Kritchenkov, Ilya S.
AU - Lezov, Alexey A.
AU - Gubarev, Alexander S.
AU - Solomatina, Anastasia I.
AU - Kachkin, Daniel V.
AU - Shcherbina, Nadezhda A.
AU - Liao, Yu Chan
AU - Liu, Yi Chun
AU - Yang, Ya Yun
AU - Tsvetkov, Nikolai V.
AU - Chelushkin, Pavel S.
AU - Chou, Pi Tai
AU - Tunik, Sergey P.
N1 - Publisher Copyright: © 2021 Elsevier Ltd
PY - 2021/10/5
Y1 - 2021/10/5
N2 - In the present report we describe block copolymer micelles based on either poly(dimethylsiloxane-block-ethylene glycol), PDMS15-b-PEG110, or poly(ε-caprolactone-block-ethylene glycol), PCL45-b-PEG110, loaded by the near-infrared (NIR) phosphorescent iridium(III) complex (Ir1) of [(N^C)2Ir(N^N)]+ type, where N^C is cyclometalating 6-(benzo[b]thiophen-2-yl)phenanthridine ligand and N^N is a bidentate diimine (1-(pyridin-2-yl)-1H-1,2,3-triazol-4-yl)methyl benzoate) ligand. We also compare both types of phosphorescent micelles (Ir1@PDMS15-b-PEG110 and Ir1@PCL45-b-PEG110) with aggregate-free non-covalent adducts of Ir1 with human serum albumin (Ir1@HSA). Finally, we evaluate the applicability of all these phosphorescent nanoparticles for oxygen sensing by phosphorescence lifetime imaging (PLIM). Both studied block copolymer micelles are compact (hydrodynamic radii less than 20 nm) and solubilize Ir1 up to at least 8 wt.% with almost 100% loading efficiency while preserving the complex phosphorescence. In contrary, the loading efficiency of Ir1 in Ir1@HSA does not exceed 27% resulting in highest possible loading of 0.35 wt.% and much lower luminescence intensity. Lifetime measurements revealed that Ir1@PCL45-b-PEG110 micelles are the best in protecting Ir1 from interactions with the components of physiological media while Ir1@PDMS15-b-PEG110 demonstrates the highest lifetime response towards oxygen variations (τdeg/τaer = 2.5), the fastest internalization into Chinese hamster ovary (CHO-K1) cell monolayers, and the strongest intracellular PLIM signal. Though neither system showed ideal combination of desired properties, the present study clearly demonstrates high potential of phosphorescent block copolymer micelles in PLIM oxygen sensing.
AB - In the present report we describe block copolymer micelles based on either poly(dimethylsiloxane-block-ethylene glycol), PDMS15-b-PEG110, or poly(ε-caprolactone-block-ethylene glycol), PCL45-b-PEG110, loaded by the near-infrared (NIR) phosphorescent iridium(III) complex (Ir1) of [(N^C)2Ir(N^N)]+ type, where N^C is cyclometalating 6-(benzo[b]thiophen-2-yl)phenanthridine ligand and N^N is a bidentate diimine (1-(pyridin-2-yl)-1H-1,2,3-triazol-4-yl)methyl benzoate) ligand. We also compare both types of phosphorescent micelles (Ir1@PDMS15-b-PEG110 and Ir1@PCL45-b-PEG110) with aggregate-free non-covalent adducts of Ir1 with human serum albumin (Ir1@HSA). Finally, we evaluate the applicability of all these phosphorescent nanoparticles for oxygen sensing by phosphorescence lifetime imaging (PLIM). Both studied block copolymer micelles are compact (hydrodynamic radii less than 20 nm) and solubilize Ir1 up to at least 8 wt.% with almost 100% loading efficiency while preserving the complex phosphorescence. In contrary, the loading efficiency of Ir1 in Ir1@HSA does not exceed 27% resulting in highest possible loading of 0.35 wt.% and much lower luminescence intensity. Lifetime measurements revealed that Ir1@PCL45-b-PEG110 micelles are the best in protecting Ir1 from interactions with the components of physiological media while Ir1@PDMS15-b-PEG110 demonstrates the highest lifetime response towards oxygen variations (τdeg/τaer = 2.5), the fastest internalization into Chinese hamster ovary (CHO-K1) cell monolayers, and the strongest intracellular PLIM signal. Though neither system showed ideal combination of desired properties, the present study clearly demonstrates high potential of phosphorescent block copolymer micelles in PLIM oxygen sensing.
KW - Oxygen sensing
KW - Polymer micelles
KW - Phosphorescence lifetime imaging
KW - Human serum albumin
KW - Block copolymers
KW - CELLS
KW - PLIM
KW - SIZE
KW - DOTS
KW - POLYMERIC MICELLES
KW - NANOPARTICLES
KW - INTERNALIZATION
KW - INTRACELLULAR OXYGEN
KW - PH
KW - PROBE
UR - http://www.scopus.com/inward/record.url?scp=85115029616&partnerID=8YFLogxK
U2 - 10.1016/j.eurpolymj.2021.110761
DO - 10.1016/j.eurpolymj.2021.110761
M3 - Article
AN - SCOPUS:85115029616
VL - 159
JO - European Polymer Journal
JF - European Polymer Journal
SN - 0014-3057
M1 - 110761
ER -
ID: 85636479