DOI

Conjugates of benzothiophene-fused azacyclononyne BT9N-NH2 with fluorescent dyes were developed to visualise azidoglycans intracellularly. The significance of the cycloalkyne core was demonstrated by comparing new reagents with DBCO- and BCN-dye conjugates. To reduce non-specificity during intracellular bioconjugation using SPAAC, less reactive BT9N-dye reagents are preferred over highly reactive DBCO- and BCN-dye conjugates.
Original languageEnglish
Pages (from-to)7637-7642
JournalOrganic and Biomolecular Chemistry
Volume22
Issue number37
DOIs
StatePublished - 3 Jul 2024

ID: 125928744