TY - JOUR

T1 - Ketamine modulates the exploratory dynamics and homebase-related behaviors of adult zebrafish

AU - Pretzel, C.W.

AU - Borba, J.V.

AU - Resmim, C.M.

AU - De Abreu, M.S.

AU - Kalueff, A.V.

AU - Fontana, B.D.

AU - Canzian, J.

AU - Rosemberg, D.B.

N1 - Export Date: 27 October 2024 CODEN: PBBHA Сведения о финансировании: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq Сведения о финансировании: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES Сведения о финансировании: Xi’an Jiaotong-Liverpool University, XJTLU Сведения о финансировании: Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, FAPERGS, 307690/2021-0, 0036/2021, 23038.007445/2021-21, 23/2551-0001853-5 Сведения о финансировании: Saint Petersburg State University, SPbU, 95443748 Текст о финансировании 1: The authors recognize the financial support and fellowships from Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior (CAPES) \u2013 Finance code 001, Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq), and Funda\u00E7\u00E3o de Amparo \u00E0 Pesquisa do Estado do Rio Grande do Sul (FAPERGS). C.W.P is recipient of PROBIC-FAPERGS fellowship grant. J.V.B. and C.M.R. are recipient of CAPES fellowship grant. B.D.F. and J.C. are recipient of CAPES PrInt and FAPERGS Post-Doc fellowship grants, respectively. D.B.R. is recipient of the CNPq research productivity grant (process number 307690/2021-0) and his work is also supported by FAPERGS (process number 23/2551-0001853-5) and PROEX/CAPES (process number 23038.007445/2021-21; grant number 0036/2021) fellowship grants. A.V.K. prior research was supported by St. Petersburg State University funds (PURE ID 95443748). The lab research is supported by the School of Science of XJTLU and the Suzhou Key Municipal Laboratory of Neurobiology and Cell Signaling at XJTLU (Suzhou, China). The funders had no role in study, design, data collection, and analysis, decision to publish, or preparation of the paper. Текст о финансировании 2: The authors recognize the financial support and fellowships from Coordena\u00E7\u00E3o de Aperfei\u00E7oamento de Pessoal de N\u00EDvel Superior (CAPES) \u2013 Finance code 001, Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq), and Funda\u00E7\u00E3o de Amparo \u00E0 Pesquisa do Estado do Rio Grande do Sul (FAPERGS). C.W.P is recipient of PROBIC-FAPERGS fellowship grant. J.V.B. and C.M.R. are recipient of CAPES fellowship grant. B.D.F. and J.C. are recipient of CAPES PrInt and FAPERGS Post-Doc fellowship grants, respectively. D.B.R. is recipient of the CNPq research productivity grant (process number 307690/2021-0 ) and his work is also supported by FAPERGS (process number 23/2551-0001853-5 ) and PROEX/CAPES (process number 23038.007445/2021-21; grant number 0036/2021 ) fellowship grants. The funders had no role in study, design, data collection, and analysis, decision to publish, or preparation of the paper.

PY - 2024/12/1

Y1 - 2024/12/1

N2 - Anxiety can be a protective emotion when animals face aversive conditions, but is commonly associated with various neuropsychiatric disorders when pathologically exacerbated. Drug repurposing has emerged as a valuable strategy based on utilizing the existing pharmaceuticals for new therapeutic purposes. Ketamine, traditionally used as an anesthetic, acts as a non-competitive antagonist of the glutamate N-methyl-D-aspartate (NMDA) receptor, and shows potential anxiolytic and antidepressant effects at subanesthetic doses. However, the influence of ketamine on multiple behavioral domains in vertebrates is not completely understood. Here, we evaluated the potential modulatory effect of ketamine on the spatio-temporal exploratory dynamics and homebase-related behaviors in adult zebrafish using the open field test (OFT). Animals were exposed to subanesthetic concentrations of ketamine (0, 2, 20, and 40 mg/L) for 20 min and their locomotion-, exploration- and homebase-related behaviors were assessed in a single 30-min trial. Our data revealed that acute ketamine (20 and 40 mg/L) induced hyperlocomotion, as verified by the increased total distance traveled. All concentrations tested elicited circling behavior, a stereotyped-like response which gradually reduced across the periods of test. We also observed modulatory effects of ketamine on the spatio-temporal exploratory pattern, in which the reduced thigmotaxis and homebase activity, associated with the increased average length of trips, suggest anxiolytic-like effects. Collectively, our findings support the modulatory effects of ketamine on the spatio-temporal exploratory activity, and corroborate the utility of homebase-related measurements to evaluate the behavioral dynamics in zebrafish models. © 2024 Elsevier Inc.

AB - Anxiety can be a protective emotion when animals face aversive conditions, but is commonly associated with various neuropsychiatric disorders when pathologically exacerbated. Drug repurposing has emerged as a valuable strategy based on utilizing the existing pharmaceuticals for new therapeutic purposes. Ketamine, traditionally used as an anesthetic, acts as a non-competitive antagonist of the glutamate N-methyl-D-aspartate (NMDA) receptor, and shows potential anxiolytic and antidepressant effects at subanesthetic doses. However, the influence of ketamine on multiple behavioral domains in vertebrates is not completely understood. Here, we evaluated the potential modulatory effect of ketamine on the spatio-temporal exploratory dynamics and homebase-related behaviors in adult zebrafish using the open field test (OFT). Animals were exposed to subanesthetic concentrations of ketamine (0, 2, 20, and 40 mg/L) for 20 min and their locomotion-, exploration- and homebase-related behaviors were assessed in a single 30-min trial. Our data revealed that acute ketamine (20 and 40 mg/L) induced hyperlocomotion, as verified by the increased total distance traveled. All concentrations tested elicited circling behavior, a stereotyped-like response which gradually reduced across the periods of test. We also observed modulatory effects of ketamine on the spatio-temporal exploratory pattern, in which the reduced thigmotaxis and homebase activity, associated with the increased average length of trips, suggest anxiolytic-like effects. Collectively, our findings support the modulatory effects of ketamine on the spatio-temporal exploratory activity, and corroborate the utility of homebase-related measurements to evaluate the behavioral dynamics in zebrafish models. © 2024 Elsevier Inc.

KW - Anxiety

KW - Ketamine

KW - NMDA receptor

KW - Open field test

KW - Spatio-temporal exploration

KW - Stereotyped behavior

UR - https://www.mendeley.com/catalogue/ec10d932-6010-322a-90b2-d98f1ed87473/

U2 - 10.1016/j.pbb.2024.173892

DO - 10.1016/j.pbb.2024.173892

M3 - статья

VL - 245

JO - Pharmacology, Biochemistry and Behavior

JF - Pharmacology, Biochemistry and Behavior

SN - 0091-3057

M1 - 173892

ER -