Research output: Contribution to journal › Article
Karyotype features of trematode Himasthla elongata. / Solovyeva, A. I.; Stefanova, V. N.; Podgornaya, O. I.; Demin, S. Iu.
In: Molecular Cytogenetics, Vol. 9, No. 1, 2016, p. 1-12.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Karyotype features of trematode Himasthla elongata
AU - Solovyeva, A. I.
AU - Stefanova, V. N.
AU - Podgornaya, O. I.
AU - Demin, S. Iu.
PY - 2016
Y1 - 2016
N2 - BACKGROUND: Trematodes have a complex life cycle with animal host changes and alternation of parthenogenetic and hermaphrodite generations. The parthenogenetic generation of the worm (rediae) from the first intermediate host Littorina littorea was used for chromosome spreads production. Karyotype description of parasitic flatworm Himasthla elongata Mehlis, 1831 (Digenea: Himasthlidae) based on fluorochrome banding and 18S rDNA mapping. RESULTS: Chromosome spreads were obtained from cercariae embryos and redial tissue suspensions with high pressure squash method.74.4 % of the analysed spreads contained 12 chromosome pairs (2n = 24). Chromosome classification was performed according to the morphometry and nomenclature published. H. elongata spread chromosomes had a rather bead-like structure. Ideograms of DAPI-banded chromosomes contained 130 individual bands. According to flow cytometry data, the H. elongata genome contains 1.25 pg of DNA, so one band contains, on average, 9.4 Mb of DNA. Image bank captures of
AB - BACKGROUND: Trematodes have a complex life cycle with animal host changes and alternation of parthenogenetic and hermaphrodite generations. The parthenogenetic generation of the worm (rediae) from the first intermediate host Littorina littorea was used for chromosome spreads production. Karyotype description of parasitic flatworm Himasthla elongata Mehlis, 1831 (Digenea: Himasthlidae) based on fluorochrome banding and 18S rDNA mapping. RESULTS: Chromosome spreads were obtained from cercariae embryos and redial tissue suspensions with high pressure squash method.74.4 % of the analysed spreads contained 12 chromosome pairs (2n = 24). Chromosome classification was performed according to the morphometry and nomenclature published. H. elongata spread chromosomes had a rather bead-like structure. Ideograms of DAPI-banded chromosomes contained 130 individual bands. According to flow cytometry data, the H. elongata genome contains 1.25 pg of DNA, so one band contains, on average, 9.4 Mb of DNA. Image bank captures of
KW - 18S rDNA mapping
KW - Digenea
KW - Himasthla elongata
KW - Karyotype
U2 - 10.1186/s13039-016-0246-8
DO - 10.1186/s13039-016-0246-8
M3 - Article
VL - 9
SP - 1
EP - 12
JO - Molecular Cytogenetics
JF - Molecular Cytogenetics
SN - 1755-8166
IS - 1
ER -
ID: 7630693