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Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles. / Stegemann, Christin; Kolobov, Alexander; Leonova, Yulia F.; Knappe, Daniel; Shamova, Olga; Ovchinnikova, Tatiana V.; Kokryakov, Vladimir N.; Hoffmann, Ralf.

In: Proteomics, Vol. 9, No. 5, 03.2009, p. 1364-1373.

Research output: Contribution to journal › Article › peer-review

Harvard

Stegemann, C, Kolobov, A, Leonova, YF, Knappe, D, Shamova, O, Ovchinnikova, TV, Kokryakov, VN & Hoffmann, R 2009, 'Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles', Proteomics, vol. 9, no. 5, pp. 1364-1373. https://doi.org/10.1002/pmic.200800569

APA

Stegemann, C., Kolobov, A., Leonova, Y. F., Knappe, D., Shamova, O., Ovchinnikova, T. V., Kokryakov, V. N., & Hoffmann, R. (2009). Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles. Proteomics, 9(5), 1364-1373. https://doi.org/10.1002/pmic.200800569

Vancouver

Stegemann C, Kolobov A, Leonova YF, Knappe D, Shamova O, Ovchinnikova TV et al. Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles. Proteomics. 2009 Mar;9(5):1364-1373. https://doi.org/10.1002/pmic.200800569

Author

Stegemann, Christin ; Kolobov, Alexander ; Leonova, Yulia F. ; Knappe, Daniel ; Shamova, Olga ; Ovchinnikova, Tatiana V. ; Kokryakov, Vladimir N. ; Hoffmann, Ralf. / Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles. In: Proteomics. 2009 ; Vol. 9, No. 5. pp. 1364-1373.

BibTeX

@article{bfc113e5214b4b7fa1de9e235b931156,
title = "Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles",
abstract = "A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond turtle Emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. It was highly active in vitro against Escherichia coli, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, and Candida albicans. The isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissociation in combination with different chemical derivatization techniques. The 40-residue peptide, called TBD-1 (turtle P-defensin 1), represents the first defensin isolated from reptilian leukocytes. It contains three disulfide bonds and shows high structural similarities to beta-defensins isolated from birds and mammals.",
keywords = "Antibacterial assay, Antimicrobial peptide, Mass spectrometry, Turtle, SOFT-SHELLED TURTLE, ANTIMICROBIAL PEPTIDES, MASS-SPECTROMETRY, INNATE IMMUNITY, ALPHA-DEFENSINS, MATRIX, ANTIBACTERIAL, ANTIBIOTICS, PROTEIN",
author = "Christin Stegemann and Alexander Kolobov and Leonova, {Yulia F.} and Daniel Knappe and Olga Shamova and Ovchinnikova, {Tatiana V.} and Kokryakov, {Vladimir N.} and Ralf Hoffmann",
year = "2009",
month = mar,
doi = "10.1002/pmic.200800569",
language = "Английский",
volume = "9",
pages = "1364--1373",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Isolation, purification and de novo sequencing of TBD-1, the first beta-defensin from leukocytes of reptiles

AU - Stegemann, Christin

AU - Kolobov, Alexander

AU - Leonova, Yulia F.

AU - Knappe, Daniel

AU - Shamova, Olga

AU - Ovchinnikova, Tatiana V.

AU - Kokryakov, Vladimir N.

AU - Hoffmann, Ralf

PY - 2009/3

Y1 - 2009/3

N2 - A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond turtle Emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. It was highly active in vitro against Escherichia coli, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, and Candida albicans. The isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissociation in combination with different chemical derivatization techniques. The 40-residue peptide, called TBD-1 (turtle P-defensin 1), represents the first defensin isolated from reptilian leukocytes. It contains three disulfide bonds and shows high structural similarities to beta-defensins isolated from birds and mammals.

AB - A novel peptide with antimicrobial activity was isolated from leukocytes of the European pond turtle Emys orbicularis and purified to homogeneity by preparative gel electrophoresis followed by reversed phase chromatography. It was highly active in vitro against Escherichia coli, Listeria monocytogenes, methicillin-resistant Staphylococcus aureus, and Candida albicans. The isolated peptide was sequenced de novo by tandem mass spectrometry using both collision-induced and electron-transfer dissociation in combination with different chemical derivatization techniques. The 40-residue peptide, called TBD-1 (turtle P-defensin 1), represents the first defensin isolated from reptilian leukocytes. It contains three disulfide bonds and shows high structural similarities to beta-defensins isolated from birds and mammals.

KW - Antibacterial assay

KW - Antimicrobial peptide

KW - Mass spectrometry

KW - Turtle

KW - SOFT-SHELLED TURTLE

KW - ANTIMICROBIAL PEPTIDES

KW - MASS-SPECTROMETRY

KW - INNATE IMMUNITY

KW - ALPHA-DEFENSINS

KW - MATRIX

KW - ANTIBACTERIAL

KW - ANTIBIOTICS

KW - PROTEIN

U2 - 10.1002/pmic.200800569

DO - 10.1002/pmic.200800569

M3 - статья

VL - 9

SP - 1364

EP - 1373

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 5

ER -

ID: 11508531