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Investigation of DNA Damage Induced by Proton and Gamma Radiation. / Kotb, O. M.; Brozhik, D. S.; Verbenko, V. N.; Gulevich, E. P.; Ezhov, V. F.; Karlin, D. L.; Pak, F. A.; Paston, S. V.; Polyanichko, A. M.; Khalikov, A. I.; Chikhirzhina, E. V.

In: Biophysics (Russian Federation), Vol. 66, No. 2, 03.2021, p. 202-208.

Research output: Contribution to journal › Article › peer-review

Harvard

Kotb, OM, Brozhik, DS, Verbenko, VN, Gulevich, EP, Ezhov, VF, Karlin, DL, Pak, FA, Paston, SV, Polyanichko, AM, Khalikov, AI & Chikhirzhina, EV 2021, 'Investigation of DNA Damage Induced by Proton and Gamma Radiation', Biophysics (Russian Federation), vol. 66, no. 2, pp. 202-208. https://doi.org/10.1134/s0006350921020123

APA

Kotb, O. M., Brozhik, D. S., Verbenko, V. N., Gulevich, E. P., Ezhov, V. F., Karlin, D. L., Pak, F. A., Paston, S. V., Polyanichko, A. M., Khalikov, A. I., & Chikhirzhina, E. V. (2021). Investigation of DNA Damage Induced by Proton and Gamma Radiation. Biophysics (Russian Federation), 66(2), 202-208. https://doi.org/10.1134/s0006350921020123

Vancouver

Kotb OM, Brozhik DS, Verbenko VN, Gulevich EP, Ezhov VF, Karlin DL et al. Investigation of DNA Damage Induced by Proton and Gamma Radiation. Biophysics (Russian Federation). 2021 Mar;66(2):202-208. https://doi.org/10.1134/s0006350921020123

Author

Kotb, O. M. ; Brozhik, D. S. ; Verbenko, V. N. ; Gulevich, E. P. ; Ezhov, V. F. ; Karlin, D. L. ; Pak, F. A. ; Paston, S. V. ; Polyanichko, A. M. ; Khalikov, A. I. ; Chikhirzhina, E. V. / Investigation of DNA Damage Induced by Proton and Gamma Radiation. In: Biophysics (Russian Federation). 2021 ; Vol. 66, No. 2. pp. 202-208.

BibTeX

@article{06a8a8c2bb354e1aad27d2c048f16b45,
title = "Investigation of DNA Damage Induced by Proton and Gamma Radiation",
abstract = "In this study, we compared the effects of gamma and high-energy proton radiation (1000 MeV) on DNA in aqueous saline solutions (5 and 150 mM NaCl) at doses of 30 and 50 Gy. We used spectral methods (the ultraviolet absorption method, spectrophotometric methods for nucleic acid quantification, spectrophotometric DNA melting, and circular dichroism) for the estimation of the number of damaged nitrogenous bases and damage of the secondary DNA structure. It was found that under these conditions, proton radiation causes more severe destruction of nitrogenous bases and the secondary DNA structure than exposure to gamma rays at the same dose. In DNA irradiated with protons, the formation of crosslinks is possible and the probability for crosslinking increases with the increase of the ionic strength of the irradiated solution.",
keywords = "destruction of nucleobases, DNA, DNA melting, gamma radiation, Keywords: radiation oncology, proton therapy",
author = "Kotb, {O. M.} and Brozhik, {D. S.} and Verbenko, {V. N.} and Gulevich, {E. P.} and Ezhov, {V. F.} and Karlin, {D. L.} and Pak, {F. A.} and Paston, {S. V.} and Polyanichko, {A. M.} and Khalikov, {A. I.} and Chikhirzhina, {E. V.}",
note = "Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Inc.",
year = "2021",
month = mar,
doi = "10.1134/s0006350921020123",
language = "English",
volume = "66",
pages = "202--208",
journal = "Biophysics (Russian Federation)",
issn = "0006-3509",
publisher = "Springer Nature",
number = "2",

}

RIS

TY - JOUR

T1 - Investigation of DNA Damage Induced by Proton and Gamma Radiation

AU - Kotb, O. M.

AU - Brozhik, D. S.

AU - Verbenko, V. N.

AU - Gulevich, E. P.

AU - Ezhov, V. F.

AU - Karlin, D. L.

AU - Pak, F. A.

AU - Paston, S. V.

AU - Polyanichko, A. M.

AU - Khalikov, A. I.

AU - Chikhirzhina, E. V.

N1 - Publisher Copyright: © 2021, Pleiades Publishing, Inc.

PY - 2021/3

Y1 - 2021/3

N2 - In this study, we compared the effects of gamma and high-energy proton radiation (1000 MeV) on DNA in aqueous saline solutions (5 and 150 mM NaCl) at doses of 30 and 50 Gy. We used spectral methods (the ultraviolet absorption method, spectrophotometric methods for nucleic acid quantification, spectrophotometric DNA melting, and circular dichroism) for the estimation of the number of damaged nitrogenous bases and damage of the secondary DNA structure. It was found that under these conditions, proton radiation causes more severe destruction of nitrogenous bases and the secondary DNA structure than exposure to gamma rays at the same dose. In DNA irradiated with protons, the formation of crosslinks is possible and the probability for crosslinking increases with the increase of the ionic strength of the irradiated solution.

AB - In this study, we compared the effects of gamma and high-energy proton radiation (1000 MeV) on DNA in aqueous saline solutions (5 and 150 mM NaCl) at doses of 30 and 50 Gy. We used spectral methods (the ultraviolet absorption method, spectrophotometric methods for nucleic acid quantification, spectrophotometric DNA melting, and circular dichroism) for the estimation of the number of damaged nitrogenous bases and damage of the secondary DNA structure. It was found that under these conditions, proton radiation causes more severe destruction of nitrogenous bases and the secondary DNA structure than exposure to gamma rays at the same dose. In DNA irradiated with protons, the formation of crosslinks is possible and the probability for crosslinking increases with the increase of the ionic strength of the irradiated solution.

KW - destruction of nucleobases

KW - DNA

KW - DNA melting

KW - gamma radiation

KW - Keywords: radiation oncology

KW - proton therapy

UR - http://www.scopus.com/inward/record.url?scp=85109166313&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/ac2746e2-7740-3beb-bfbd-aca97af0a870/

U2 - 10.1134/s0006350921020123

DO - 10.1134/s0006350921020123

M3 - Article

AN - SCOPUS:85109166313

VL - 66

SP - 202

EP - 208

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

SN - 0006-3509

IS - 2

ER -

ID: 87708468