TY - JOUR

T1 - Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles

AU - Gorshkov, Oleg

AU - Chernova, Tatyana

AU - Mokshina, Natalia

AU - Gogoleva, Natalia

AU - Suslov, Dmitry

AU - Tkachenko, Alexander

AU - Gorshkova, Tatyana

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

AB - Phloem fibers are important elements of plant architecture and the target product of many fiber crops. A key stage in fiber development is intrusive elongation, the mechanisms of which are largely unknown. Integrated analysis of miRNA and mRNA expression profiles in intrusivelygrowing fibers obtained by laser microdissection from flax (Linum usitatissimum L.) stem revealed all 124 known flax miRNA from23 gene families and the potential targets of differentially expressed miRNAs. A comparison of the expression between phloem fibers at different developmental stages, and parenchyma and xylem tissues demonstrated that members of miR159, miR166, miR167, miR319, miR396 families were down-regulated in intrusively growing fibers. Some putative target genes of these miRNA families, such as those putatively encoding growth-regulating factors, an argonaute family protein, and a homeobox-leucine zipper family protein were up-regulated in elongating fibers. miR160, miR169, miR390, andmiR394 showed increased expression. Changes in the expression levels of miRNAs and their target genes did not match expectations for the majority of predicted target genes. Taken together, poorly understood intrusive fiber elongation, the key process of phloem fiber development, was characterized from a miRNA-target point of view, giving new insights into its regulation.

KW - Flax

KW - Intrusive growth

KW - Laser microdissection

KW - MiRNA

KW - Phloem fibers

KW - Transcriptome

KW - LINUM-USITATISSIMUM

KW - ARABIDOPSIS-THALIANA

KW - READ ALIGNMENT

KW - IN-SILICO IDENTIFICATION

KW - RESPONSE REGULATORS

KW - transcriptome

KW - CELL-PROLIFERATION

KW - MICRORNAS

KW - flax

KW - intrusive growth

KW - GENE-EXPRESSION

KW - phloem fibers

KW - TARGETS

KW - laser microdissection

KW - miRNA

KW - DIFFERENTIATION

UR - http://www.scopus.com/inward/record.url?scp=85064281690&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/intrusive-growth-phloem-fibers-flax-stem-integrated-analysis-mirna-mrna-expression-profiles

U2 - 10.3390/plants8020047

DO - 10.3390/plants8020047

M3 - Article

C2 - 30791461

VL - 8

JO - Plants

JF - Plants

SN - 2223-7747

IS - 2

M1 - 47

ER -