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Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy. / Shakirova, Julia R. ; Baigildin, Vadim A. ; Solomatina, Anastasia I. ; Babadi Aghakhanpour, Reza ; Pavlovskiy, Vladimir V. ; Porsev, Vitaly V. ; Tunik, Sergey P. .

In: Advanced Functional Materials, 22.12.2022.

Research output: Contribution to journal › Article › peer-review

Harvard

Shakirova, JR, Baigildin, VA, Solomatina, AI, Babadi Aghakhanpour, R, Pavlovskiy, VV, Porsev, VV & Tunik, SP 2022, 'Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy', Advanced Functional Materials.

APA

Shakirova, J. R., Baigildin, V. A., Solomatina, A. I., Babadi Aghakhanpour, R., Pavlovskiy, V. V., Porsev, V. V., & Tunik, S. P. (2022). Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy. Advanced Functional Materials.

Vancouver

Shakirova JR, Baigildin VA, Solomatina AI, Babadi Aghakhanpour R, Pavlovskiy VV, Porsev VV et al. Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy. Advanced Functional Materials. 2022 Dec 22.

Author

Shakirova, Julia R. ; Baigildin, Vadim A. ; Solomatina, Anastasia I. ; Babadi Aghakhanpour, Reza ; Pavlovskiy, Vladimir V. ; Porsev, Vitaly V. ; Tunik, Sergey P. . / Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy. In: Advanced Functional Materials. 2022.

BibTeX

@article{2b8234d819764aabac27522118e0a943,
title = "Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy",
abstract = "Herein, a novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported. To prevent oxygen quenching of phosphorescence and to improve the probe biocompatibility, the complex is covalently conjugated with a water-soluble block-copolymer that also increases its pH sensitivity. The resulting polymeric nanoprobe demonstrates a strong response of the phosphorescence lifetime onto pH variations in physiological range. Cellular experiments with Chinese hamster ovary (CHO-K1) cells show the predominant internalization of the probe in acidified cell compartments, endosomes and lysosomes. The analysis of phosphorescence lifetime imaging microscopy data confirms applicability of the sensor for monitoring of intra- and extracellular pH in cell cultures.",
author = "Shakirova, {Julia R.} and Baigildin, {Vadim A.} and Solomatina, {Anastasia I.} and {Babadi Aghakhanpour}, Reza and Pavlovskiy, {Vladimir V.} and Porsev, {Vitaly V.} and Tunik, {Sergey P.}",
year = "2022",
month = dec,
day = "22",
language = "English",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Intracellular pH sensor based on heteroleptic bis-cyclometalated iridium(III) complex embedded into block-copolymer nanospecies: application in phosphorescence lifetime imaging microscopy

AU - Shakirova, Julia R.

AU - Baigildin, Vadim A.

AU - Solomatina, Anastasia I.

AU - Babadi Aghakhanpour, Reza

AU - Pavlovskiy, Vladimir V.

AU - Porsev, Vitaly V.

AU - Tunik, Sergey P.

PY - 2022/12/22

Y1 - 2022/12/22

N2 - Herein, a novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported. To prevent oxygen quenching of phosphorescence and to improve the probe biocompatibility, the complex is covalently conjugated with a water-soluble block-copolymer that also increases its pH sensitivity. The resulting polymeric nanoprobe demonstrates a strong response of the phosphorescence lifetime onto pH variations in physiological range. Cellular experiments with Chinese hamster ovary (CHO-K1) cells show the predominant internalization of the probe in acidified cell compartments, endosomes and lysosomes. The analysis of phosphorescence lifetime imaging microscopy data confirms applicability of the sensor for monitoring of intra- and extracellular pH in cell cultures.

AB - Herein, a novel pH-responsive phosphorescent probe based on cyclometalated iridium(III) complex is reported. To prevent oxygen quenching of phosphorescence and to improve the probe biocompatibility, the complex is covalently conjugated with a water-soluble block-copolymer that also increases its pH sensitivity. The resulting polymeric nanoprobe demonstrates a strong response of the phosphorescence lifetime onto pH variations in physiological range. Cellular experiments with Chinese hamster ovary (CHO-K1) cells show the predominant internalization of the probe in acidified cell compartments, endosomes and lysosomes. The analysis of phosphorescence lifetime imaging microscopy data confirms applicability of the sensor for monitoring of intra- and extracellular pH in cell cultures.

UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202212390

M3 - Article

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

ER -

ID: 100931767