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Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. / Bogdanova, Maria; Zabirnyk, Arsenii; Malashicheva, Anna; Enayati, Katarina Zihlavnikova; Karlsen, Tommy Aleksander; Kaljusto, Mari Liis; Kvitting, John Peder Escobar; Dissen, Erik; Sullivan, Gareth John; Kostareva, Anna; Stensløkken, Kåre Olav; Rutkovskiy, Arkady; Vaage, Jarle.

In: Scientific Reports, Vol. 9, No. 1, 12934, 10.09.2019.

Research output: Contribution to journal › Article › peer-review

Harvard

Bogdanova, M, Zabirnyk, A, Malashicheva, A, Enayati, KZ, Karlsen, TA, Kaljusto, ML, Kvitting, JPE, Dissen, E, Sullivan, GJ, Kostareva, A, Stensløkken, KO, Rutkovskiy, A & Vaage, J 2019, 'Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties', Scientific Reports, vol. 9, no. 1, 12934. https://doi.org/10.1038/s41598-019-49016-0

APA

Bogdanova, M., Zabirnyk, A., Malashicheva, A., Enayati, K. Z., Karlsen, T. A., Kaljusto, M. L., Kvitting, J. P. E., Dissen, E., Sullivan, G. J., Kostareva, A., Stensløkken, K. O., Rutkovskiy, A., & Vaage, J. (2019). Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. Scientific Reports, 9(1), [12934]. https://doi.org/10.1038/s41598-019-49016-0

Vancouver

Bogdanova M, Zabirnyk A, Malashicheva A, Enayati KZ, Karlsen TA, Kaljusto ML et al. Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. Scientific Reports. 2019 Sep 10;9(1). 12934. https://doi.org/10.1038/s41598-019-49016-0

Author

Bogdanova, Maria ; Zabirnyk, Arsenii ; Malashicheva, Anna ; Enayati, Katarina Zihlavnikova ; Karlsen, Tommy Aleksander ; Kaljusto, Mari Liis ; Kvitting, John Peder Escobar ; Dissen, Erik ; Sullivan, Gareth John ; Kostareva, Anna ; Stensløkken, Kåre Olav ; Rutkovskiy, Arkady ; Vaage, Jarle. / Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. In: Scientific Reports. 2019 ; Vol. 9, No. 1.

BibTeX

@article{6a794bb9fd2f4242822e231ed7d2671a,
title = "Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties",
abstract = "Valve interstitial cells (VICs) are crucial in the development of calcific aortic valve disease. The purpose of the present investigation was to compare the phenotype, differentiation potential and stem cell-like properties of cells from calcified and healthy aortic valves. VICs were isolated from human healthy and calcified aortic valves. Calcification was induced with osteogenic medium. Unlike VICs from healthy valves, VICs from calcified valves cultured without osteogenic medium stained positively for calcium deposits with Alizarin Red confirming their calcific phenotype. Stimulation of VICs from calcified valves with osteogenic medium increased calcification (p = 0.02), but not significantly different from healthy VICs. When stimulated with myofibroblastic medium, VICs from calcified valves had lower expression of myofibroblastic markers, measured by flow cytometry and RT-qPCR, compared to healthy VICs. Contraction of collagen gel (a measure of myofibroblastic activity) was attenuated in cells from calcified valves (p = 0.04). Moreover, VICs from calcified valves, unlike cells from healthy valves had lower potential to differentiate into adipogenic pathway and lower expression of stem cell-associated markers CD106 (p = 0.04) and aldehyde dehydrogenase (p = 0.04). In conclusion, VICs from calcified aortic have reduced multipotency compared to cells from healthy valves, which should be considered when investigating possible medical treatments of aortic valve calcification.",
keywords = "ALDEHYDE DEHYDROGENASE, 3-DIMENSIONAL CULTURE, DISEASE, CALCIFICATION, EXPRESSION, STENOSIS, MECHANISMS, PHENOTYPE, INFLAMMATION",
author = "Maria Bogdanova and Arsenii Zabirnyk and Anna Malashicheva and Enayati, {Katarina Zihlavnikova} and Karlsen, {Tommy Aleksander} and Kaljusto, {Mari Liis} and Kvitting, {John Peder Escobar} and Erik Dissen and Sullivan, {Gareth John} and Anna Kostareva and Stensl{\o}kken, {K{\aa}re Olav} and Arkady Rutkovskiy and Jarle Vaage",
note = "Bogdanova, M., Zabirnyk, A., Malashicheva, A. et al. Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. Sci Rep 9, 12934 (2019) doi:10.1038/s41598-019-49016-0",
year = "2019",
month = sep,
day = "10",
doi = "10.1038/s41598-019-49016-0",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties

AU - Bogdanova, Maria

AU - Zabirnyk, Arsenii

AU - Malashicheva, Anna

AU - Enayati, Katarina Zihlavnikova

AU - Karlsen, Tommy Aleksander

AU - Kaljusto, Mari Liis

AU - Kvitting, John Peder Escobar

AU - Dissen, Erik

AU - Sullivan, Gareth John

AU - Kostareva, Anna

AU - Stensløkken, Kåre Olav

AU - Rutkovskiy, Arkady

AU - Vaage, Jarle

N1 - Bogdanova, M., Zabirnyk, A., Malashicheva, A. et al. Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties. Sci Rep 9, 12934 (2019) doi:10.1038/s41598-019-49016-0

PY - 2019/9/10

Y1 - 2019/9/10

N2 - Valve interstitial cells (VICs) are crucial in the development of calcific aortic valve disease. The purpose of the present investigation was to compare the phenotype, differentiation potential and stem cell-like properties of cells from calcified and healthy aortic valves. VICs were isolated from human healthy and calcified aortic valves. Calcification was induced with osteogenic medium. Unlike VICs from healthy valves, VICs from calcified valves cultured without osteogenic medium stained positively for calcium deposits with Alizarin Red confirming their calcific phenotype. Stimulation of VICs from calcified valves with osteogenic medium increased calcification (p = 0.02), but not significantly different from healthy VICs. When stimulated with myofibroblastic medium, VICs from calcified valves had lower expression of myofibroblastic markers, measured by flow cytometry and RT-qPCR, compared to healthy VICs. Contraction of collagen gel (a measure of myofibroblastic activity) was attenuated in cells from calcified valves (p = 0.04). Moreover, VICs from calcified valves, unlike cells from healthy valves had lower potential to differentiate into adipogenic pathway and lower expression of stem cell-associated markers CD106 (p = 0.04) and aldehyde dehydrogenase (p = 0.04). In conclusion, VICs from calcified aortic have reduced multipotency compared to cells from healthy valves, which should be considered when investigating possible medical treatments of aortic valve calcification.

AB - Valve interstitial cells (VICs) are crucial in the development of calcific aortic valve disease. The purpose of the present investigation was to compare the phenotype, differentiation potential and stem cell-like properties of cells from calcified and healthy aortic valves. VICs were isolated from human healthy and calcified aortic valves. Calcification was induced with osteogenic medium. Unlike VICs from healthy valves, VICs from calcified valves cultured without osteogenic medium stained positively for calcium deposits with Alizarin Red confirming their calcific phenotype. Stimulation of VICs from calcified valves with osteogenic medium increased calcification (p = 0.02), but not significantly different from healthy VICs. When stimulated with myofibroblastic medium, VICs from calcified valves had lower expression of myofibroblastic markers, measured by flow cytometry and RT-qPCR, compared to healthy VICs. Contraction of collagen gel (a measure of myofibroblastic activity) was attenuated in cells from calcified valves (p = 0.04). Moreover, VICs from calcified valves, unlike cells from healthy valves had lower potential to differentiate into adipogenic pathway and lower expression of stem cell-associated markers CD106 (p = 0.04) and aldehyde dehydrogenase (p = 0.04). In conclusion, VICs from calcified aortic have reduced multipotency compared to cells from healthy valves, which should be considered when investigating possible medical treatments of aortic valve calcification.

KW - ALDEHYDE DEHYDROGENASE

KW - 3-DIMENSIONAL CULTURE

KW - DISEASE

KW - CALCIFICATION

KW - EXPRESSION

KW - STENOSIS

KW - MECHANISMS

KW - PHENOTYPE

KW - INFLAMMATION

UR - http://www.scopus.com/inward/record.url?scp=85072018040&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-49016-0

DO - 10.1038/s41598-019-49016-0

M3 - Article

C2 - 31506459

AN - SCOPUS:85072018040

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 12934

ER -

ID: 49027659