Interaction of macrophage migration inhibitory factor with ceruloplasmin: role of labile copper ions. / Kostevich, V.A.; Sokolov, A.V.; Grudinina, N.A.; Zakharova, E.T.; Samygina, V.R.; Vasilyev, V.B.
In: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 28, No. 5, 2015, p. 817-826.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Interaction of macrophage migration inhibitory factor with ceruloplasmin: role of labile copper ions
AU - Kostevich, V.A.
AU - Sokolov, A.V.
AU - Grudinina, N.A.
AU - Zakharova, E.T.
AU - Samygina, V.R.
AU - Vasilyev, V.B.
PY - 2015
Y1 - 2015
N2 - Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is a target for pharmacological treatment of sepsis and malignant tumors. Inhibition of tautomerase activity of MIF in reaction with p-hydroxyphenylpyruvate (HPP) was observed in the presence of ceruloplasmin (CP), a copper-containing plasma protein. Binding labile copper ions to CP (CP+Cu(II)) is a prerequisite for MIF inhibiting. CP+Cu(II) is shown to be an uncompetitive inhibitor of MIF (Ki ~ 37 nM), which suggests formation of a complex ‘MIF-HPP-CP-Cu(II)’. Filtration of CP+Cu(II) on a column with Chelex-100, otherwise the presence of high concentrations of histidine, cysteine or methionine abrogated the inhibitory effect of CP. Adding salts of Co(II) and Ni(II) that replace copper ions in the labile sites prevented the inhibitory effect of CP+Cu(II). Limited proteolysis of CP by thrombin diminished its oxidase activity in reaction with p-phenylenediamine, but endowed it with the capacity of inhibiting MIF. Covalent modification of
AB - Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is a target for pharmacological treatment of sepsis and malignant tumors. Inhibition of tautomerase activity of MIF in reaction with p-hydroxyphenylpyruvate (HPP) was observed in the presence of ceruloplasmin (CP), a copper-containing plasma protein. Binding labile copper ions to CP (CP+Cu(II)) is a prerequisite for MIF inhibiting. CP+Cu(II) is shown to be an uncompetitive inhibitor of MIF (Ki ~ 37 nM), which suggests formation of a complex ‘MIF-HPP-CP-Cu(II)’. Filtration of CP+Cu(II) on a column with Chelex-100, otherwise the presence of high concentrations of histidine, cysteine or methionine abrogated the inhibitory effect of CP. Adding salts of Co(II) and Ni(II) that replace copper ions in the labile sites prevented the inhibitory effect of CP+Cu(II). Limited proteolysis of CP by thrombin diminished its oxidase activity in reaction with p-phenylenediamine, but endowed it with the capacity of inhibiting MIF. Covalent modification of
KW - Ceruloplasmin
KW - Macrophage migration inhibitory factor
KW - Protein–protein interaction
KW - Tautomerase activity
KW - p-Hydroxyphenylpyruvate
U2 - 10.1007/s10534-015-9868-2
DO - 10.1007/s10534-015-9868-2
M3 - Article
VL - 28
SP - 817
EP - 826
JO - BioMetals
JF - BioMetals
SN - 0966-0844
IS - 5
ER -
ID: 3946304