TY - JOUR

T1 - Interaction of fullerene C60 with bovine serum albumin at the water – air interface

AU - Noskov, B. A.

AU - Isakov, N. A.

AU - Gochev, G.

AU - Loglio, G.

AU - Miller, R.

N1 - Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - This work is directed to the elucidation of the peculiarities of fullerene C60 interactions with bovine serum albumin (BSA) in mixed layers at the water-air interface. To this aim, the dilational surface elasticity was measured as a function of surface pressure and surface age and optical methods together with the atomic force microscopy (AFM) were applied. The dependencies of the dilational dynamic surface elasticity of the mixed C60/BSA layers on the surface pressure have two local maxima indicating a conformational transition in the layer. At low surface pressures (region of the first maximum) the surface properties are determined mainly by the protein, while in the second region of relatively high surface pressures the contribution of the fullerene is more important. The AFM images show that both C60 layers and mixed C60/BSA layers contain separate large fullerene aggregates with the length in Z-directions of up to 100 nm but the morphology of the regions between these aggregates differs for the two systems. The mixed layer contains some patches of a network of almost merged fullerene/protein aggregates with a length in Z-direction not longer than about 20 nm. The formation and the subsequent reorganization of the network lead to non-monotonic kinetic dependences of the dynamic surface elasticity in the course of protein penetration into the fullerene layer. The obtained results show strong interactions between the components in the surface layer and thereby can change the toxicity of the fullerene.

AB - This work is directed to the elucidation of the peculiarities of fullerene C60 interactions with bovine serum albumin (BSA) in mixed layers at the water-air interface. To this aim, the dilational surface elasticity was measured as a function of surface pressure and surface age and optical methods together with the atomic force microscopy (AFM) were applied. The dependencies of the dilational dynamic surface elasticity of the mixed C60/BSA layers on the surface pressure have two local maxima indicating a conformational transition in the layer. At low surface pressures (region of the first maximum) the surface properties are determined mainly by the protein, while in the second region of relatively high surface pressures the contribution of the fullerene is more important. The AFM images show that both C60 layers and mixed C60/BSA layers contain separate large fullerene aggregates with the length in Z-directions of up to 100 nm but the morphology of the regions between these aggregates differs for the two systems. The mixed layer contains some patches of a network of almost merged fullerene/protein aggregates with a length in Z-direction not longer than about 20 nm. The formation and the subsequent reorganization of the network lead to non-monotonic kinetic dependences of the dynamic surface elasticity in the course of protein penetration into the fullerene layer. The obtained results show strong interactions between the components in the surface layer and thereby can change the toxicity of the fullerene.

KW - Aggregation

KW - Atomic force microscopy

KW - Bovine Serum Albumin

KW - Fullerene

KW - Oscillating barrier

KW - Surface dilational rheology

KW - CARBON NANOMATERIALS

KW - NANOPARTICLES

KW - DILATIONAL SURFACE VISCOELASTICITY

KW - COLLAPSE

KW - ADSORBED FILMS

KW - PROTEINS

KW - BINDING

UR - http://www.scopus.com/inward/record.url?scp=85117198598&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfa.2021.127702

DO - 10.1016/j.colsurfa.2021.127702

M3 - Article

AN - SCOPUS:85117198598

VL - 631

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - 127702

ER -