Research output: Contribution to journal › Article › peer-review
INTERACTION OF DNA WITH COORDINATION-COMPOUNDS OF DIVALENT PLATINUM .1. EFFECT OF CIS-DIAMINODICHLOROPLATINUM ON DNA MOLECULAR-PARAMETERS IN SOLUTION. / KASYANENKO, NA; VALUEVA, SV; SMORYGO, NA; DYANCHENKO, SA; FRISMAN, EV.
In: Molecular Biology, Vol. 29, No. 2, 1995, p. 200-203.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - INTERACTION OF DNA WITH COORDINATION-COMPOUNDS OF DIVALENT PLATINUM .1. EFFECT OF CIS-DIAMINODICHLOROPLATINUM ON DNA MOLECULAR-PARAMETERS IN SOLUTION
AU - KASYANENKO, NA
AU - VALUEVA, SV
AU - SMORYGO, NA
AU - DYANCHENKO, SA
AU - FRISMAN, EV
PY - 1995
Y1 - 1995
N2 - The interaction of DNA with cis-diaminodichloroplatinum at different ionic strengths was studied by dynamic birefringence, viscosimetry, and circular dichroism. Although cis-diaminodichloroplatinum is a nonelectrolyte, it can bind to DNA by electrostatic interaction. It was found that the mechanism of complex formation is determined by the [Pt]/[DNA] ratio. At a low concentration, cis-diaminodichloroplatinum formed complexes with the phosphate groups of DNA, while at a higher one it bound to the nucleic bases. As this took place, the secondary structure of DNA did not change. An increase of cis-diaminodichloroplatinum concentration disrupted the stacking contacts and denatured the DNA. It was assumed that the stability of the complex is ensured by incorporation of the nitrogen-containing groups of the bases into the platinum first coordination sphere. The phosphates of DNA served as counterions in the outer coordination
AB - The interaction of DNA with cis-diaminodichloroplatinum at different ionic strengths was studied by dynamic birefringence, viscosimetry, and circular dichroism. Although cis-diaminodichloroplatinum is a nonelectrolyte, it can bind to DNA by electrostatic interaction. It was found that the mechanism of complex formation is determined by the [Pt]/[DNA] ratio. At a low concentration, cis-diaminodichloroplatinum formed complexes with the phosphate groups of DNA, while at a higher one it bound to the nucleic bases. As this took place, the secondary structure of DNA did not change. An increase of cis-diaminodichloroplatinum concentration disrupted the stacking contacts and denatured the DNA. It was assumed that the stability of the complex is ensured by incorporation of the nitrogen-containing groups of the bases into the platinum first coordination sphere. The phosphates of DNA served as counterions in the outer coordination
KW - CIS-DIAMINODICHLOROPLATINUM
KW - DNA-PLATINUM COMPLEX
M3 - статья
VL - 29
SP - 200
EP - 203
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 2
ER -
ID: 73400497