TY - JOUR

T1 - Influence of Iron Oxide-Based Nanoparticles with Various Shell Modifications on the Generation of Reactive Oxygen Species in Stimulated Human Blood Cells in vitro

AU - Toropova, Ya G.

AU - Gorshkova, M. N.

AU - Motorina, D. S.

AU - Korolev, D. V.

AU - Skorik, Yu. A.

AU - Shulmeister, G. A.

AU - Podyacheva, E. Yu.

AU - Bagrov , A. Ya.

N1 - Toropova, Y.G., Gorshkova, M.N., Motorina, D.S. et al. Influence of Iron Oxide-Based Nanoparticles with Various Shell Modifications on the Generation of Reactive Oxygen Species in Stimulated Human Blood Cells in vitro. J Evol Biochem Phys 57, 782–791 (2021). https://doi.org/10.1134/S0022093021040049

PY - 2021/7/1

Y1 - 2021/7/1

N2 - Among the available nanoparticles, those based on iron oxide are of particular interest due to their biological safety, magnetic properties, potential for imparting desired physico-chemical properties, and capacity for surface modifications with biocompatible, bioactive materials, ligands and antibodies. An important aspect of nanoparticles that determines their possibilities and range of clinical applications is their biocompatibility profile. Toxic effects can arise due to mechanisms mediated by reactive oxygen species (ROS); therefore, the development of nanoparticles intended for biomedical applications requires special attention to safety assessment in terms of the generation of oxidative stress. In this study, we examined the application of various shells (based on polylactide, polysaccharide, or albumin) on the dose-dependent effect of magnetite nanoparticles (MNPs) on the generation of ROS in stimulated human blood cells, as well as on the dynamics of the induced oxidative hemolysis of erythrocytes. MNPs with a shell of polylactide, albumin, and polysaccharide, in the range of all the used concentrations (0.1–2.0 mg/mL) and throughout the entire incubation period (0–180 min), did not affect the kinetics of the chemiluminescence response, while providing a unidirectional but differently pronounced decrease in the maximum intensity of induced chemiluminescence and total ROS production. All types of investigated nanoparticles in the range of concentrations from 1.0 to 2.0 mg/mL provided a dose-dependent enhancement of this effect. Under conditions of induced ROS generation, the various MNP shells did not modify the effects of these nanoparticles and only regulated their intensity. MNPs with a polylactide shell had a maximum effect.

AB - Among the available nanoparticles, those based on iron oxide are of particular interest due to their biological safety, magnetic properties, potential for imparting desired physico-chemical properties, and capacity for surface modifications with biocompatible, bioactive materials, ligands and antibodies. An important aspect of nanoparticles that determines their possibilities and range of clinical applications is their biocompatibility profile. Toxic effects can arise due to mechanisms mediated by reactive oxygen species (ROS); therefore, the development of nanoparticles intended for biomedical applications requires special attention to safety assessment in terms of the generation of oxidative stress. In this study, we examined the application of various shells (based on polylactide, polysaccharide, or albumin) on the dose-dependent effect of magnetite nanoparticles (MNPs) on the generation of ROS in stimulated human blood cells, as well as on the dynamics of the induced oxidative hemolysis of erythrocytes. MNPs with a shell of polylactide, albumin, and polysaccharide, in the range of all the used concentrations (0.1–2.0 mg/mL) and throughout the entire incubation period (0–180 min), did not affect the kinetics of the chemiluminescence response, while providing a unidirectional but differently pronounced decrease in the maximum intensity of induced chemiluminescence and total ROS production. All types of investigated nanoparticles in the range of concentrations from 1.0 to 2.0 mg/mL provided a dose-dependent enhancement of this effect. Under conditions of induced ROS generation, the various MNP shells did not modify the effects of these nanoparticles and only regulated their intensity. MNPs with a polylactide shell had a maximum effect.

KW - magnetite nanoparticles

KW - shell

KW - whole blood

KW - induced chemiluminescence

KW - hemolysis

KW - ROS

KW - dose dependence

KW - TOXICITY

KW - MAGNETIC NANOPARTICLES

UR - https://www.mendeley.com/catalogue/273e3d7a-c7ff-3dd2-a477-b3d247e753f0/

U2 - 10.1134/s0022093021040049

DO - 10.1134/s0022093021040049

M3 - Article

VL - 57

SP - 782

EP - 791

JO - Journal of Evolutionary Biochemistry and Physiology

JF - Journal of Evolutionary Biochemistry and Physiology

SN - 0022-0930

IS - 4

ER -