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Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth. / Marchenkova, M. A.; Konarev, P. V.; Boikova, A. S.; Ilina, K. B.; Pisarevsky, Yu V.; Kovalchuk, M. V.

In: Crystallography Reports, Vol. 66, No. 5, 01.09.2021, p. 751-757.

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Marchenkova, M. A. ; Konarev, P. V. ; Boikova, A. S. ; Ilina, K. B. ; Pisarevsky, Yu V. ; Kovalchuk, M. V. / Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth. In: Crystallography Reports. 2021 ; Vol. 66, No. 5. pp. 751-757.

BibTeX

@article{526756b2873e48cc8ab5f498b50932e5,
title = "Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth",
abstract = "Abstract: The influence of the precipitant type (LiCl, NaCl, KCl, NiCl2, and CuCl2) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to grow crystals in order to reveal the influence of the oligomeric composition on the crystal growth. The data obtained in this and previous studies on the influence of precipitant concentration yield an inversely proportional dependence of the total content of octamers and dimers on the cation atomic number, which is in agreement with the increase in the ion activity in the lyotropic series for Li+, Na+, and K+ and the increase in the ionic radius for Li+, Na+, K+, Ni2+, and Cu2+. It is shown that a decrease in the protein concentration in a crystallization solution leads to a decrease in octamer volume fraction at an invariable volume fraction of dimers and reduces the probability of crystal formation.",
keywords = "SMALL-ANGLE SCATTERING, STAGE, MACROMOLECULES, BEAMLINE",
author = "Marchenkova, {M. A.} and Konarev, {P. V.} and Boikova, {A. S.} and Ilina, {K. B.} and Pisarevsky, {Yu V.} and Kovalchuk, {M. V.}",
note = "Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Inc.",
year = "2021",
month = sep,
day = "1",
doi = "10.1134/s1063774521050138",
language = "English",
volume = "66",
pages = "751--757",
journal = "Crystallography Reports",
issn = "1063-7745",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "5",

}

RIS

TY - JOUR

T1 - Influence of Chlorides of Mono- and Divalent Metals on the Oligomeric Composition of Lysozyme Crystallization Solutions and Further Crystal Growth

AU - Marchenkova, M. A.

AU - Konarev, P. V.

AU - Boikova, A. S.

AU - Ilina, K. B.

AU - Pisarevsky, Yu V.

AU - Kovalchuk, M. V.

N1 - Publisher Copyright: © 2021, Pleiades Publishing, Inc.

PY - 2021/9/1

Y1 - 2021/9/1

N2 - Abstract: The influence of the precipitant type (LiCl, NaCl, KCl, NiCl2, and CuCl2) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to grow crystals in order to reveal the influence of the oligomeric composition on the crystal growth. The data obtained in this and previous studies on the influence of precipitant concentration yield an inversely proportional dependence of the total content of octamers and dimers on the cation atomic number, which is in agreement with the increase in the ion activity in the lyotropic series for Li+, Na+, and K+ and the increase in the ionic radius for Li+, Na+, K+, Ni2+, and Cu2+. It is shown that a decrease in the protein concentration in a crystallization solution leads to a decrease in octamer volume fraction at an invariable volume fraction of dimers and reduces the probability of crystal formation.

AB - Abstract: The influence of the precipitant type (LiCl, NaCl, KCl, NiCl2, and CuCl2) on the formation of oligomers (dimers and octamers) in lysozyme crystallization solutions at two protein concentrations has been investigated by small-angle X-ray scattering (SAXS). The same solutions have been used to grow crystals in order to reveal the influence of the oligomeric composition on the crystal growth. The data obtained in this and previous studies on the influence of precipitant concentration yield an inversely proportional dependence of the total content of octamers and dimers on the cation atomic number, which is in agreement with the increase in the ion activity in the lyotropic series for Li+, Na+, and K+ and the increase in the ionic radius for Li+, Na+, K+, Ni2+, and Cu2+. It is shown that a decrease in the protein concentration in a crystallization solution leads to a decrease in octamer volume fraction at an invariable volume fraction of dimers and reduces the probability of crystal formation.

KW - SMALL-ANGLE SCATTERING

KW - STAGE

KW - MACROMOLECULES

KW - BEAMLINE

UR - http://www.scopus.com/inward/record.url?scp=85116397331&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/42e8c082-17be-324a-9766-2ba5c6173f8a/

U2 - 10.1134/s1063774521050138

DO - 10.1134/s1063774521050138

M3 - Article

AN - SCOPUS:85116397331

VL - 66

SP - 751

EP - 757

JO - Crystallography Reports

JF - Crystallography Reports

SN - 1063-7745

IS - 5

ER -

ID: 88195152