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Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells. / Sutula, Gleb I. ; Alhasan, Bashar A.; Vorobev, Mikhail L. ; Guzhova, Irina V. ; Suvorova, Irina I. .

In: Biochemical and Biophysical Research Communications, Vol. 532, No. 2, 05.11.2020, p. 280-284.

Research output: Contribution to specialist publication › Article › Popular Science

Harvard

Sutula, GI, Alhasan, BA, Vorobev, ML, Guzhova, IV & Suvorova, II 2020, 'Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells' Biochemical and Biophysical Research Communications, vol. 532, no. 2, pp. 280-284. https://doi.org/10.1016/j.bbrc.2020.07.133

APA

Sutula, G. I., Alhasan, B. A., Vorobev, M. L., Guzhova, I. V., & Suvorova, I. I. (2020). Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells. Biochemical and Biophysical Research Communications, 532(2), 280-284. https://doi.org/10.1016/j.bbrc.2020.07.133

Vancouver

Sutula GI, Alhasan BA, Vorobev ML, Guzhova IV, Suvorova II. Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells. Biochemical and Biophysical Research Communications. 2020 Nov 5;532(2):280-284. https://doi.org/10.1016/j.bbrc.2020.07.133

Author

Sutula, Gleb I. ; Alhasan, Bashar A. ; Vorobev, Mikhail L. ; Guzhova, Irina V. ; Suvorova, Irina I. . / Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells. In: Biochemical and Biophysical Research Communications. 2020 ; Vol. 532, No. 2. pp. 280-284.

BibTeX

@misc{7bd2876a0d814cca83944422711cdb2e,
title = "Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells",
abstract = "Defective pluripotent cells are removed from embryos prior to differentiation, presumably due to upregulation of the p53 pathway. However, the mechanism underlying p53 protein activation is still unknown. Embryonic stem cells (ESCs), corresponding to cells of the preimplantation blastocyst, likely have similar mechanisms for abnormal cell elimination. Using a mouse ESC cell line with inducible ulk1 gene expression, we showed that Ulk1 upregulation is accompanied by p53 phosphorylation on Ser15. ESCs tolerated the activated p53 and did not undergo apoptosis or cell cycle blockade upon Ulk1 overexpression. However, massive cell death was observed after retinoic acid treatment, suggesting a role of Ulk1-induced p53 activation in the elimination of defective pluripotent cells prior to differentiation.",
keywords = "embryonic stem cells, Pluripotency, p53, mTORм, AMPK/Ulk1 pathway, autophagy, mTOR, Embryonic stem cells, Autophagy, AUTOPHAGY",
author = "Sutula, {Gleb I.} and Alhasan, {Bashar A.} and Vorobev, {Mikhail L.} and Guzhova, {Irina V.} and Suvorova, {Irina I.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = nov,
day = "5",
doi = "10.1016/j.bbrc.2020.07.133",
language = "English",
volume = "532",
pages = "280--284",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",

}

RIS

TY - GEN

T1 - Inducible Ulk1 expression activates the p53 protein in mouse embryonic stem cells

AU - Sutula, Gleb I.

AU - Alhasan, Bashar A.

AU - Vorobev, Mikhail L.

AU - Guzhova, Irina V.

AU - Suvorova, Irina I.

N1 - Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/11/5

Y1 - 2020/11/5

N2 - Defective pluripotent cells are removed from embryos prior to differentiation, presumably due to upregulation of the p53 pathway. However, the mechanism underlying p53 protein activation is still unknown. Embryonic stem cells (ESCs), corresponding to cells of the preimplantation blastocyst, likely have similar mechanisms for abnormal cell elimination. Using a mouse ESC cell line with inducible ulk1 gene expression, we showed that Ulk1 upregulation is accompanied by p53 phosphorylation on Ser15. ESCs tolerated the activated p53 and did not undergo apoptosis or cell cycle blockade upon Ulk1 overexpression. However, massive cell death was observed after retinoic acid treatment, suggesting a role of Ulk1-induced p53 activation in the elimination of defective pluripotent cells prior to differentiation.

AB - Defective pluripotent cells are removed from embryos prior to differentiation, presumably due to upregulation of the p53 pathway. However, the mechanism underlying p53 protein activation is still unknown. Embryonic stem cells (ESCs), corresponding to cells of the preimplantation blastocyst, likely have similar mechanisms for abnormal cell elimination. Using a mouse ESC cell line with inducible ulk1 gene expression, we showed that Ulk1 upregulation is accompanied by p53 phosphorylation on Ser15. ESCs tolerated the activated p53 and did not undergo apoptosis or cell cycle blockade upon Ulk1 overexpression. However, massive cell death was observed after retinoic acid treatment, suggesting a role of Ulk1-induced p53 activation in the elimination of defective pluripotent cells prior to differentiation.

KW - embryonic stem cells

KW - Pluripotency

KW - p53

KW - mTORм

KW - AMPK/Ulk1 pathway

KW - autophagy

KW - mTOR

KW - Embryonic stem cells

KW - Autophagy

KW - AUTOPHAGY

UR - http://www.scopus.com/inward/record.url?scp=85089995393&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/b3f754f6-f70e-3c8b-adb4-e1846895605c/

U2 - 10.1016/j.bbrc.2020.07.133

DO - 10.1016/j.bbrc.2020.07.133

M3 - Article

VL - 532

SP - 280

EP - 284

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -

ID: 70042250