Research output: Contribution to journal › Article › peer-review
We have earlier characterized Saccharomyces cerevisiae strains with mutations of essential SUP45 and SUP35, which code for translation termination factors eRF1 and eRF3, respectively. In this work, the sup45 and sup35 nonsense mutants were compared with respect to the levels of eight tRNAs: tRNA Tyr, tRNAGln, tRNATrp, tRNALeu, tRNAArg (described as potential suppressor tRNAs), tRNA Pro, tRNAHis, and tRNAGly. The mutants did not display a selective increase in tRNAs, capable of a noncanonical read-through at stop codons. Most of the mutations increased the level of all tRNAs under study. The mechanisms providing for the viability of the sup45 and sup35 nonsense mutants are discussed.
Original language | English |
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Pages (from-to) | 647-653 |
Number of pages | 7 |
Journal | Molecular Biology |
Volume | 40 |
Issue number | 4 |
DOIs | |
State | Published - Jul 2006 |
ID: 70378862