Research output: Contribution to journal › Article › peer-review
In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles. / Toropova, Yana G.; Golovkin, Alexey S.; Malashicheva, Anna B.; Korolev, Dmitry V.; Gorshkov, Andrey N.; Gareev, Kamil G.; Afonin, Michael V.; Galagudza, Michael M.
In: International Journal of Nanomedicine, Vol. 12, 2017, p. 593-603.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles
AU - Toropova, Yana G.
AU - Golovkin, Alexey S.
AU - Malashicheva, Anna B.
AU - Korolev, Dmitry V.
AU - Gorshkov, Andrey N.
AU - Gareev, Kamil G.
AU - Afonin, Michael V.
AU - Galagudza, Michael M.
PY - 2017
Y1 - 2017
N2 - Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, FemOn-SiO2 composite flake-like, and SiO2-FemOn core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 mu g. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. FemOn-SiO2 composite flake-like and SiO2-FemOn core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects.
AB - Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, FemOn-SiO2 composite flake-like, and SiO2-FemOn core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 mu g. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. FemOn-SiO2 composite flake-like and SiO2-FemOn core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects.
KW - iron oxide nanoparticles
KW - composite nanoparticles
KW - silica coating
KW - silica nanoflakes
KW - cytotoxicity
KW - IRON-OXIDE NANOPARTICLES
KW - BIOMEDICAL APPLICATIONS
KW - CONTRAST AGENT
KW - CELLS
KW - CYTOTOXICITY
KW - DELIVERY
KW - BIOCOMPATIBILITY
KW - FIBROBLASTS
KW - INDUCTION
KW - DESIGN
U2 - 10.2147/IJN.S122580
DO - 10.2147/IJN.S122580
M3 - статья
VL - 12
SP - 593
EP - 603
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
SN - 1176-9114
ER -
ID: 9458151