TY - JOUR

T1 - Improved osseointegration properties of hierarchical microtopographic/nanotopographic coatings fabricated on titanium implants

AU - Земцова, Елена Георгиевна

AU - Смирнов, Владимир Михайлович

AU - Морозов, Павел Евгеньевич

AU - Юдинцева, Н.М.

AU - Валиев, Руслан Зуфарович

AU - Шевцов, МА

N1 - Funding Information: The authors are grateful to Leonid I Potokin for SEM studies and Olga G Genbach, Nelly V Koroleva, and Dmitriy Gavr-ilov for assistance in animal experiments. The assessment of the nanocoated samples was performed at the resource center at the Scientific Park of the St Petersburg State University (Nanotechnology, X-ray Diffraction Studies, Innovative Technologies of Composite Nanomaterials). The study was supported by a grant from the Russian Science Foundation (Grant No 14-50-00068) and by the Federal Agency of Scientific Organizations, Russia, by State Grant of the Ministry of Health of the Russian Federation (No 32).

PY - 2018/4

Y1 - 2018/4

N2 - Background: Titanium (Ti) implants are extensively used in reconstructive surgery and orthopedics. However, the intrinsic inertness of untreated Ti implants usually results in insufficient osseointegration. In order to improve the osteoconductivity properties of the implants, they are coated with hierarchical microtopographic/nanotopographic coatings employing the method of molecular layering of atomic layer deposition (ML-ALD). Results: The analysis of the fabricated nanostructured relief employing scanning electron microscopy, atomic force microscopy, and electron spectroscopy for chemical analysis clearly demonstrated the formation of the nanotopographic (<100 nm) and microtopographic (0.1-0.5 µm) titano-organic structures on the surface of the nanograined Ti implants. Subsequent coincubation of the MC3T3-E1 mouse osteoblasts on the microtopographic/nanotopographic surface of the implants resulted in enhanced osteogenic cell differentiation (the production of alkaline phosphatase, osteopontin, and osteocalcin). In vivo assessment of the osseointegrative properties of the microtopographically/nanotopographically coated implants in a model of below-knee amputation in New Zealand rabbits demonstrated enhanced new bone formation in the zone of the bone-implant contact (as measured by X-ray study) and increased osseointegration strength (removal torque measurements). Conclusion: The fabrication of the hierarchical microtopographic/nanotopographic coatings on the nanograined Ti implants significantly improves the osseointegrative properties of the intraosseous Ti implants. This effect could be employed in both translational and clinical studies in orthopedic and reconstructive surgery.

AB - Background: Titanium (Ti) implants are extensively used in reconstructive surgery and orthopedics. However, the intrinsic inertness of untreated Ti implants usually results in insufficient osseointegration. In order to improve the osteoconductivity properties of the implants, they are coated with hierarchical microtopographic/nanotopographic coatings employing the method of molecular layering of atomic layer deposition (ML-ALD). Results: The analysis of the fabricated nanostructured relief employing scanning electron microscopy, atomic force microscopy, and electron spectroscopy for chemical analysis clearly demonstrated the formation of the nanotopographic (<100 nm) and microtopographic (0.1-0.5 µm) titano-organic structures on the surface of the nanograined Ti implants. Subsequent coincubation of the MC3T3-E1 mouse osteoblasts on the microtopographic/nanotopographic surface of the implants resulted in enhanced osteogenic cell differentiation (the production of alkaline phosphatase, osteopontin, and osteocalcin). In vivo assessment of the osseointegrative properties of the microtopographically/nanotopographically coated implants in a model of below-knee amputation in New Zealand rabbits demonstrated enhanced new bone formation in the zone of the bone-implant contact (as measured by X-ray study) and increased osseointegration strength (removal torque measurements). Conclusion: The fabrication of the hierarchical microtopographic/nanotopographic coatings on the nanograined Ti implants significantly improves the osseointegrative properties of the intraosseous Ti implants. This effect could be employed in both translational and clinical studies in orthopedic and reconstructive surgery.

KW - Hierarchical coatings

KW - MC3T3-E1 osteoblasts

KW - Microtopographic/nanotopographic coatings

KW - Molecular layering

KW - Osseointegration

KW - Titanium implants

KW - Torque

KW - Male

KW - Titanium/chemistry

KW - Osteoblasts/cytology

KW - Bone-Implant Interface/physiology

KW - Surface Properties

KW - Cell Differentiation

KW - Rabbits

KW - Microscopy, Electron, Scanning

KW - Nanostructures/chemistry

KW - Coated Materials, Biocompatible/chemistry

KW - Prostheses and Implants

KW - Bone Regeneration

KW - Osseointegration/drug effects

KW - Animals

KW - Mice

KW - Osteocalcin/metabolism

KW - Alkaline Phosphatase/metabolism

KW - Osteogenesis

KW - OSTEOBLAST DIFFERENTIATION

KW - TOPOGRAPHY

KW - VIVO BONE RESPONSE

KW - PROLIFERATION

KW - FATE

KW - IN-VITRO

KW - NANOSTRUCTURES

KW - osseointegration

KW - MESENCHYMAL STEM-CELLS

KW - NANOTOPOGRAPHY

KW - titanium implants

KW - hierarchical coatings

KW - microtopographic/nanotopographic coatings

KW - molecular layering

KW - SURFACES

UR - http://www.scopus.com/inward/record.url?scp=85045407427&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/improved-osseointegration-properties-hierarchical-microtopographicnanotopographic-coatings-fabricate

U2 - 10.2147/IJN.S161292

DO - 10.2147/IJN.S161292

M3 - Article

C2 - 29692612

VL - 13

SP - 2175

EP - 2188

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

IS - 11

ER -