TY - JOUR

T1 - Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection

AU - Sitkov, Nikita

AU - Ryabko, Andrey

AU - Kolobov, Alexey

AU - Maximov, Alexsandr

AU - Moshnikov, Vyacheslav

AU - Pshenichnyuk , Stanislav

AU - Komolov, Alexei

AU - Aleshin, Andrey

AU - Zimina, Tatiana

N1 - Sitkov, N.; Ryabko, A.; Kolobov, A.; Maximov, A.; Moshnikov, V.; Pshenichnyuk, S.; Komolov, A.; Aleshin, A.; Zimina, T. Impedimetric Biosensor Coated with Zinc Oxide Nanorods Synthesized by a Modification of the Hydrothermal Method for Antibody Detection. Chemosensors 2023, 11, 66. https://doi.org/10.3390/chemosensors11010066

PY - 2023/1/13

Y1 - 2023/1/13

N2 - Impedimetric biosensors are used for detecting a wide range of analytes. The detection principle is a perspective for the development of new types of analytical devices for biomolecular diagnosis of diseases. Of particular interest are biosensors with very high sensitivities, capable of detecting trace amounts of biomarkers or drugs in biological fluids. Impedimetric biosensors possess a potential for increased sensitivity, since their electrodes can be modified with nanostructured materials, in particular zinc oxide. In this work, a miniature biosensor with an array of zinc oxide nanorods synthesized by the hydrothermal method has been created. Protein A was immobilized on the resulting structure, which was previously tested for binding to omalizumab by capillary electrophoresis. Using impedance spectroscopy, it was possible to detect the binding of omalizumab at concentrations down to 5 pg/mL. The resulting structures are suitable for creating reusable biosensor systems, since ZnO-coated electrodes are easily cleaned by photocatalytic decomposition of the bound molecules. The biosensor is promising for use in Point-of-Care systems designed for fast, multimodal detection of molecular markers of a wide range of diseases.

AB - Impedimetric biosensors are used for detecting a wide range of analytes. The detection principle is a perspective for the development of new types of analytical devices for biomolecular diagnosis of diseases. Of particular interest are biosensors with very high sensitivities, capable of detecting trace amounts of biomarkers or drugs in biological fluids. Impedimetric biosensors possess a potential for increased sensitivity, since their electrodes can be modified with nanostructured materials, in particular zinc oxide. In this work, a miniature biosensor with an array of zinc oxide nanorods synthesized by the hydrothermal method has been created. Protein A was immobilized on the resulting structure, which was previously tested for binding to omalizumab by capillary electrophoresis. Using impedance spectroscopy, it was possible to detect the binding of omalizumab at concentrations down to 5 pg/mL. The resulting structures are suitable for creating reusable biosensor systems, since ZnO-coated electrodes are easily cleaned by photocatalytic decomposition of the bound molecules. The biosensor is promising for use in Point-of-Care systems designed for fast, multimodal detection of molecular markers of a wide range of diseases.

KW - impedimetric biosensor

KW - zinc oxide

KW - express detection

KW - nanorods

KW - antibodies

KW - label-free detection

KW - hydrothermal synthesis

UR - https://www.mendeley.com/catalogue/8d347864-f2d3-3b83-82d2-2b6da52f7bb8/

U2 - 10.3390/chemosensors11010066

DO - 10.3390/chemosensors11010066

M3 - Article

VL - 11

JO - Chemosensors

JF - Chemosensors

SN - 2227-9040

IS - 1

M1 - 66

ER -