Spectroscopic measurements of transition frequencies in various atomic systems are a significant part of modern physics. They enable the testing of fundamental interactions, the determination of physical constants, and the study of fundamental symmetries that occur in nature with unprecedented accuracy. Studies based on two-photon spectroscopy of simple atoms represent some of the most accurate experiments to date. The verification of precision experimental results is largely supported by theoretical analysis, which is most rigorous for light, nonrelativistic atoms and ions. In the last decade, much attention in the literature has been paid to the study of the quantum interference effect (QIE) in hydrogen and hydrogenlike atomic systems. This has made it possible to significantly reduce the experimental error in determining the appropriate transition frequency. The theoretical description of the QIE corresponds to the consideration of similar pathways arising for close-lying resonant states into which the transition frequency is measured. In the present work, the influence of the emission process on the absorption profile formation is investigated. The results of the studies carried out in this work show the need to take into account the effect of quantum interference in cascade radiation for the precise determination of the absorption transition frequency to highly excited states in two-photon spectroscopic experiments.