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Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia. / Murashev, Boris; Kazennova, Elena; Kozlov, Alexander; Murasheva, Irina; Dukhovlinova, Elena; Galachyants, Yuri; Dorofeeva, Ekaterina; Dukhovlinov, Ilya; Smirnova, Galina; Masharsky, Alexey; Klimov, Nikolay; Kozlov, Andrei P.

In: Biotechnology Journal, Vol. 2, No. 7, 01.07.2007, p. 871-878.

Research output: Contribution to journal › Article › peer-review

Harvard

Murashev, B, Kazennova, E, Kozlov, A, Murasheva, I, Dukhovlinova, E, Galachyants, Y, Dorofeeva, E, Dukhovlinov, I, Smirnova, G, Masharsky, A, Klimov, N & Kozlov, AP 2007, 'Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia', Biotechnology Journal, vol. 2, no. 7, pp. 871-878. https://doi.org/10.1002/biot.200700024

APA

Murashev, B., Kazennova, E., Kozlov, A., Murasheva, I., Dukhovlinova, E., Galachyants, Y., Dorofeeva, E., Dukhovlinov, I., Smirnova, G., Masharsky, A., Klimov, N., & Kozlov, A. P. (2007). Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia. Biotechnology Journal, 2(7), 871-878. https://doi.org/10.1002/biot.200700024

Vancouver

Murashev B, Kazennova E, Kozlov A, Murasheva I, Dukhovlinova E, Galachyants Y et al. Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia. Biotechnology Journal. 2007 Jul 1;2(7):871-878. https://doi.org/10.1002/biot.200700024

Author

Murashev, Boris ; Kazennova, Elena ; Kozlov, Alexander ; Murasheva, Irina ; Dukhovlinova, Elena ; Galachyants, Yuri ; Dorofeeva, Ekaterina ; Dukhovlinov, Ilya ; Smirnova, Galina ; Masharsky, Alexey ; Klimov, Nikolay ; Kozlov, Andrei P. / Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia. In: Biotechnology Journal. 2007 ; Vol. 2, No. 7. pp. 871-878.

BibTeX

@article{ab8d8ae6942e451d9af5c690cd6def2b,
title = "Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia",
abstract = "Human immunodeficiency virus (HIV)-1 subtype A strains circulating among the majority of HIV-infected individuals in the former Soviet Union (FSU) countries demonstrate low genetic diversity. The consensus sequence of the FSU region-specific isolate has been used for the candidate DNA vaccine development. We constructed recombinant plasmids with four viral genes: env (gp140), gag, pol (reverse transcriptase), and nef. We immunized BALB/c mice intramuscularly using equimolar mixture of four recombinant plasmids, and observed significant cytotoxic T lymphocyte response and specific CD8+ cell production against cells presenting HIV-1 peptides. Overall, the Th1 pathway of immune response clearly dominated. Immunological properties of this candidate DNA vaccine against HIV-1 suggest the possibility of its further study in clinical trials.",
keywords = "DNA vaccine, Flow cytometry-based CTL assay, H2-D-restricted CTL epitopes, HIV-1 subtype A, Intracellular cytokine staining",
author = "Boris Murashev and Elena Kazennova and Alexander Kozlov and Irina Murasheva and Elena Dukhovlinova and Yuri Galachyants and Ekaterina Dorofeeva and Ilya Dukhovlinov and Galina Smirnova and Alexey Masharsky and Nikolay Klimov and Kozlov, {Andrei P.}",
year = "2007",
month = jul,
day = "1",
doi = "10.1002/biot.200700024",
language = "English",
volume = "2",
pages = "871--878",
journal = "Biotechnology Journal",
issn = "1860-6768",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - Immunogenicity of candidate DNA vaccine based on subtype A of human immunodeficiency virus type 1 predominant in Russia

AU - Murashev, Boris

AU - Kazennova, Elena

AU - Kozlov, Alexander

AU - Murasheva, Irina

AU - Dukhovlinova, Elena

AU - Galachyants, Yuri

AU - Dorofeeva, Ekaterina

AU - Dukhovlinov, Ilya

AU - Smirnova, Galina

AU - Masharsky, Alexey

AU - Klimov, Nikolay

AU - Kozlov, Andrei P.

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Human immunodeficiency virus (HIV)-1 subtype A strains circulating among the majority of HIV-infected individuals in the former Soviet Union (FSU) countries demonstrate low genetic diversity. The consensus sequence of the FSU region-specific isolate has been used for the candidate DNA vaccine development. We constructed recombinant plasmids with four viral genes: env (gp140), gag, pol (reverse transcriptase), and nef. We immunized BALB/c mice intramuscularly using equimolar mixture of four recombinant plasmids, and observed significant cytotoxic T lymphocyte response and specific CD8+ cell production against cells presenting HIV-1 peptides. Overall, the Th1 pathway of immune response clearly dominated. Immunological properties of this candidate DNA vaccine against HIV-1 suggest the possibility of its further study in clinical trials.

AB - Human immunodeficiency virus (HIV)-1 subtype A strains circulating among the majority of HIV-infected individuals in the former Soviet Union (FSU) countries demonstrate low genetic diversity. The consensus sequence of the FSU region-specific isolate has been used for the candidate DNA vaccine development. We constructed recombinant plasmids with four viral genes: env (gp140), gag, pol (reverse transcriptase), and nef. We immunized BALB/c mice intramuscularly using equimolar mixture of four recombinant plasmids, and observed significant cytotoxic T lymphocyte response and specific CD8+ cell production against cells presenting HIV-1 peptides. Overall, the Th1 pathway of immune response clearly dominated. Immunological properties of this candidate DNA vaccine against HIV-1 suggest the possibility of its further study in clinical trials.

KW - DNA vaccine

KW - Flow cytometry-based CTL assay

KW - H2-D-restricted CTL epitopes

KW - HIV-1 subtype A

KW - Intracellular cytokine staining

UR - http://www.scopus.com/inward/record.url?scp=34547470055&partnerID=8YFLogxK

U2 - 10.1002/biot.200700024

DO - 10.1002/biot.200700024

M3 - Article

C2 - 17582822

AN - SCOPUS:34547470055

VL - 2

SP - 871

EP - 878

JO - Biotechnology Journal

JF - Biotechnology Journal

SN - 1860-6768

IS - 7

ER -

ID: 37699242