Research output: Contribution to journal › Article
Imbalance in memory B-cells in patients with pulmonary sarcoidosis. / Zinchenko, U.; Kudryavtsev, I.; Starshinova, A.; Basantsova, N.; Serebriakova, M.; Malkova, A.; Toubi, E.; Churilov, L.; Yablonskiy, P.
In: European Respiratory Journal, Supplement, Vol. 54, No. S63, 2019, p. PA4096.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Imbalance in memory B-cells in patients with pulmonary sarcoidosis.
AU - Zinchenko, U.
AU - Kudryavtsev, I.
AU - Starshinova, A.
AU - Basantsova, N.
AU - Serebriakova, M.
AU - Malkova, A.
AU - Toubi, E.
AU - Churilov, L.
AU - Yablonskiy, P.
PY - 2019
Y1 - 2019
N2 - Introduction: Sarcoidosis (SD) is considered to be a T-cell-driven disorder, but recent studies show also B-cells participation in SD (Musaelyan, 2018; Saussine, 2012). The aim was to determine the different subsets of memory B-cells in SD. Methods: A prospective comparative study was conducted in 2016-2018 with examination of patients with SD (n=37) and healthy control (HC) (n=35). 200 μL of whole blood were stained on surface with fluorochrome-conjugated monoclonal antibodies: anti-IgD Alexa Fluor 488 (clone IA6-2, isotype - Mouse IgG2a, κ), anti-CD38 PE (clone LS198-4-3, isotype - IgG1 Mouse). IgD, CD19 - BioLegend, Inc. (USA), CD38 - Beckman Coulter, Inc (USA). Sample acquisition was performed with a Navios flow cytometer (Beckman Coulter, Inc., USA). Supported by Russian Federation Government Grant (contract N14.W03.31.0009 of 13.02.2017). Results: The frequency of B cells in SD was significantly higher than in HC (14.48% (9.86; 17.29) vs 11.09% (8.52; 13.28), p=0.008). “Activated naive” Bm2 cells and pr
AB - Introduction: Sarcoidosis (SD) is considered to be a T-cell-driven disorder, but recent studies show also B-cells participation in SD (Musaelyan, 2018; Saussine, 2012). The aim was to determine the different subsets of memory B-cells in SD. Methods: A prospective comparative study was conducted in 2016-2018 with examination of patients with SD (n=37) and healthy control (HC) (n=35). 200 μL of whole blood were stained on surface with fluorochrome-conjugated monoclonal antibodies: anti-IgD Alexa Fluor 488 (clone IA6-2, isotype - Mouse IgG2a, κ), anti-CD38 PE (clone LS198-4-3, isotype - IgG1 Mouse). IgD, CD19 - BioLegend, Inc. (USA), CD38 - Beckman Coulter, Inc (USA). Sample acquisition was performed with a Navios flow cytometer (Beckman Coulter, Inc., USA). Supported by Russian Federation Government Grant (contract N14.W03.31.0009 of 13.02.2017). Results: The frequency of B cells in SD was significantly higher than in HC (14.48% (9.86; 17.29) vs 11.09% (8.52; 13.28), p=0.008). “Activated naive” Bm2 cells and pr
M3 - Article
VL - 54
SP - PA4096
JO - European Respiratory Journal, Supplement
JF - European Respiratory Journal, Supplement
SN - 0904-1850
IS - S63
ER -
ID: 78450385