Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry

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Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry. / Razgonova, Mayya P.; Burlyaeva, Marina O.; Zinchenko, Yulia N.; Krylova, Ekaterina A.; Chunikhina, Olga A.; Ivanova, Natalia M.; Zakharenko, Alexander M.; Golokhvast, Kirill S.

In: Plants, Vol. 11, No. 16, 2147, 18.08.2022.

Research output: Contribution to journal › Article › peer-review

Author

Razgonova, Mayya P. ; Burlyaeva, Marina O. ; Zinchenko, Yulia N. ; Krylova, Ekaterina A. ; Chunikhina, Olga A. ; Ivanova, Natalia M. ; Zakharenko, Alexander M. ; Golokhvast, Kirill S. / Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry. In: Plants. 2022 ; Vol. 11, No. 16.

BibTeX

@article{420c0424f5114b67b31e5a790439c2a8,

title = "Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry",

abstract = "The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China.",

keywords = "Vigna unguiculata, bioactive substances, grain cultivar, laser microscopy, local landrace, metabolites, seed, tandem mass spectrometry, vegetable cultivar",

author = "Razgonova, {Mayya P.} and Burlyaeva, {Marina O.} and Zinchenko, {Yulia N.} and Krylova, {Ekaterina A.} and Chunikhina, {Olga A.} and Ivanova, {Natalia M.} and Zakharenko, {Alexander M.} and Golokhvast, {Kirill S.}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = aug,

day = "18",

doi = "doi:10.3390/plants11162147",

language = "English",

volume = "11",

journal = "Plants",

issn = "2223-7747",

publisher = "MDPI AG",

number = "16",

}

RIS

TY - JOUR

T1 - Identification and Spatial Distribution of Bioactive Compounds in Seeds Vigna unguiculata (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry

AU - Razgonova, Mayya P.

AU - Burlyaeva, Marina O.

AU - Zinchenko, Yulia N.

AU - Krylova, Ekaterina A.

AU - Chunikhina, Olga A.

AU - Ivanova, Natalia M.

AU - Zakharenko, Alexander M.

AU - Golokhvast, Kirill S.

PY - 2022/8/18

Y1 - 2022/8/18

N2 - The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China.

AB - The research presents a comparative metabolomic study of extracts of Vigna unguiculata seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups sesquipedalis (vegetable accessions) and unguiculata (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in V. unguiculata seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group sesquipedalis, and 24 in unguiculata. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China.

KW - Vigna unguiculata

KW - bioactive substances

KW - grain cultivar

KW - laser microscopy

KW - local landrace

KW - metabolites

KW - seed

KW - tandem mass spectrometry

KW - vegetable cultivar

UR - http://www.scopus.com/inward/record.url?scp=85137346345&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/f921ffd4-cea9-3981-a4aa-717da6ac64d3/

U2 - doi:10.3390/plants11162147

DO - doi:10.3390/plants11162147

M3 - Article

C2 - 36015450

VL - 11

JO - Plants

JF - Plants

SN - 2223-7747

IS - 16

M1 - 2147

ER -

ID: 98734583