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Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin. / Sokolov, Alexej V.; Ageeva, Kira V.; Cherkalina, Olga S.; Pulina, Maria O.; Zakharova, Elena T.; Prozorovskii, Vladimir N.; Aksenov, Denis V.; Vasilyev, Vadim B.; Panasenko, Oleg M.

In: Chemistry and Physics of Lipids, Vol. 163, No. 4-5, 05.2010, p. 347-355.

Research output: Contribution to journalArticlepeer-review

Harvard

Sokolov, AV, Ageeva, KV, Cherkalina, OS, Pulina, MO, Zakharova, ET, Prozorovskii, VN, Aksenov, DV, Vasilyev, VB & Panasenko, OM 2010, 'Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin', Chemistry and Physics of Lipids, vol. 163, no. 4-5, pp. 347-355. https://doi.org/10.1016/j.chemphyslip.2010.02.002

APA

Sokolov, A. V., Ageeva, K. V., Cherkalina, O. S., Pulina, M. O., Zakharova, E. T., Prozorovskii, V. N., Aksenov, D. V., Vasilyev, V. B., & Panasenko, O. M. (2010). Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin. Chemistry and Physics of Lipids, 163(4-5), 347-355. https://doi.org/10.1016/j.chemphyslip.2010.02.002

Vancouver

Sokolov AV, Ageeva KV, Cherkalina OS, Pulina MO, Zakharova ET, Prozorovskii VN et al. Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin. Chemistry and Physics of Lipids. 2010 May;163(4-5):347-355. https://doi.org/10.1016/j.chemphyslip.2010.02.002

Author

Sokolov, Alexej V. ; Ageeva, Kira V. ; Cherkalina, Olga S. ; Pulina, Maria O. ; Zakharova, Elena T. ; Prozorovskii, Vladimir N. ; Aksenov, Denis V. ; Vasilyev, Vadim B. ; Panasenko, Oleg M. / Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin. In: Chemistry and Physics of Lipids. 2010 ; Vol. 163, No. 4-5. pp. 347-355.

BibTeX

@article{0cbf281c323f4037bba4700b62f74227,
title = "Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin",
abstract = "The first evidence of multi-component complexes formed by myeloperoxidase (MPO), ceruloplasmin (CP), and very low/low density lipoproteins (VLDL/LDL) obtained by electrophoresis, gel filtration, and photon-correlation spectroscopy (PCS) is presented in this paper. Complexes were observed when isolated MPO, CP, and VLDL/LDL were mixed and/or when MPO was added to the blood plasma. Complex LDL-MPO-CP was detected in 44 of 100 plasma samples taken from patients with atherosclerosis, and 33 of 44 samples also contained the VLDL-MPO-CP complex. MPO concentration in these patients' plasma exceeded 800 ng/ml. Interaction of MPO with high density lipoproteins (HDL) was not revealed, as well as binding of CP to lipoproteins in the absence of MPO. Adding antibodies against apoB-100 to VLDL-MPO-CP and LDL-MPO-CP complexes results in release of lipoproteins. Using PCS the diameters of complexes under study were evaluated. By comparing concentrations of the components in complexes formed by MPO, CP, and lipoproteins their stoichiometry was assessed as 2VLDL:1MPO:2CP and 1LDL:1MPO:2CP. Lipoproteins affected the inhibition of MPO peroxidase activity by CP. The affinity of lipoproteins to MPO-CP complex was assessed using apparent dissociation constants determined as ∼0.3 nM for VLDL and ∼0.14 nM for LDL.",
keywords = "apoB-100, Ceruloplasmin, Human blood lipoproteins, Myeloperoxidase, Photon-correlation spectroscopy, Protein-protein interactions",
author = "Sokolov, {Alexej V.} and Ageeva, {Kira V.} and Cherkalina, {Olga S.} and Pulina, {Maria O.} and Zakharova, {Elena T.} and Prozorovskii, {Vladimir N.} and Aksenov, {Denis V.} and Vasilyev, {Vadim B.} and Panasenko, {Oleg M.}",
year = "2010",
month = may,
doi = "10.1016/j.chemphyslip.2010.02.002",
language = "English",
volume = "163",
pages = "347--355",
journal = "Chemistry and Physics of Lipids",
issn = "0009-3084",
publisher = "Elsevier",
number = "4-5",

}

RIS

TY - JOUR

T1 - Identification and properties of complexes formed by myeloperoxidase with lipoproteins and ceruloplasmin

AU - Sokolov, Alexej V.

AU - Ageeva, Kira V.

AU - Cherkalina, Olga S.

AU - Pulina, Maria O.

AU - Zakharova, Elena T.

AU - Prozorovskii, Vladimir N.

AU - Aksenov, Denis V.

AU - Vasilyev, Vadim B.

AU - Panasenko, Oleg M.

PY - 2010/5

Y1 - 2010/5

N2 - The first evidence of multi-component complexes formed by myeloperoxidase (MPO), ceruloplasmin (CP), and very low/low density lipoproteins (VLDL/LDL) obtained by electrophoresis, gel filtration, and photon-correlation spectroscopy (PCS) is presented in this paper. Complexes were observed when isolated MPO, CP, and VLDL/LDL were mixed and/or when MPO was added to the blood plasma. Complex LDL-MPO-CP was detected in 44 of 100 plasma samples taken from patients with atherosclerosis, and 33 of 44 samples also contained the VLDL-MPO-CP complex. MPO concentration in these patients' plasma exceeded 800 ng/ml. Interaction of MPO with high density lipoproteins (HDL) was not revealed, as well as binding of CP to lipoproteins in the absence of MPO. Adding antibodies against apoB-100 to VLDL-MPO-CP and LDL-MPO-CP complexes results in release of lipoproteins. Using PCS the diameters of complexes under study were evaluated. By comparing concentrations of the components in complexes formed by MPO, CP, and lipoproteins their stoichiometry was assessed as 2VLDL:1MPO:2CP and 1LDL:1MPO:2CP. Lipoproteins affected the inhibition of MPO peroxidase activity by CP. The affinity of lipoproteins to MPO-CP complex was assessed using apparent dissociation constants determined as ∼0.3 nM for VLDL and ∼0.14 nM for LDL.

AB - The first evidence of multi-component complexes formed by myeloperoxidase (MPO), ceruloplasmin (CP), and very low/low density lipoproteins (VLDL/LDL) obtained by electrophoresis, gel filtration, and photon-correlation spectroscopy (PCS) is presented in this paper. Complexes were observed when isolated MPO, CP, and VLDL/LDL were mixed and/or when MPO was added to the blood plasma. Complex LDL-MPO-CP was detected in 44 of 100 plasma samples taken from patients with atherosclerosis, and 33 of 44 samples also contained the VLDL-MPO-CP complex. MPO concentration in these patients' plasma exceeded 800 ng/ml. Interaction of MPO with high density lipoproteins (HDL) was not revealed, as well as binding of CP to lipoproteins in the absence of MPO. Adding antibodies against apoB-100 to VLDL-MPO-CP and LDL-MPO-CP complexes results in release of lipoproteins. Using PCS the diameters of complexes under study were evaluated. By comparing concentrations of the components in complexes formed by MPO, CP, and lipoproteins their stoichiometry was assessed as 2VLDL:1MPO:2CP and 1LDL:1MPO:2CP. Lipoproteins affected the inhibition of MPO peroxidase activity by CP. The affinity of lipoproteins to MPO-CP complex was assessed using apparent dissociation constants determined as ∼0.3 nM for VLDL and ∼0.14 nM for LDL.

KW - apoB-100

KW - Ceruloplasmin

KW - Human blood lipoproteins

KW - Myeloperoxidase

KW - Photon-correlation spectroscopy

KW - Protein-protein interactions

UR - http://www.scopus.com/inward/record.url?scp=77951022464&partnerID=8YFLogxK

U2 - 10.1016/j.chemphyslip.2010.02.002

DO - 10.1016/j.chemphyslip.2010.02.002

M3 - Article

C2 - 20167214

AN - SCOPUS:77951022464

VL - 163

SP - 347

EP - 355

JO - Chemistry and Physics of Lipids

JF - Chemistry and Physics of Lipids

SN - 0009-3084

IS - 4-5

ER -

ID: 97808241