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Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris. / Ehrlich, Hermann; Kaluzhnaya, Oksana V.; Brunner, Eike; Tsurkan, Mikhail V.; Ereskovsky, Alexander; Ilan, Micha; Tabachnick, Konstantin R.; Bazhenov, Vasilii V.; Paasch, Silvia; Kammer, Martin; Born, René; Stelling, Allison; Galli, Roberta; Belikov, Sergei; Petrova, Olga V.; Sivkov, Victor V.; Vyalikh, Denis; Hunoldt, Sebastian; Wörheide, Gert.

In: Journal of Structural Biology, Vol. 183, No. 3, 09.2013, p. 474-483.

Research output: Contribution to journal › Article › peer-review

Harvard

Ehrlich, H, Kaluzhnaya, OV, Brunner, E, Tsurkan, MV, Ereskovsky, A, Ilan, M, Tabachnick, KR, Bazhenov, VV, Paasch, S, Kammer, M, Born, R, Stelling, A, Galli, R, Belikov, S, Petrova, OV, Sivkov, VV, Vyalikh, D, Hunoldt, S & Wörheide, G 2013, 'Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris', Journal of Structural Biology, vol. 183, no. 3, pp. 474-483. https://doi.org/10.1016/j.jsb.2013.06.015

APA

Ehrlich, H., Kaluzhnaya, O. V., Brunner, E., Tsurkan, M. V., Ereskovsky, A., Ilan, M., Tabachnick, K. R., Bazhenov, V. V., Paasch, S., Kammer, M., Born, R., Stelling, A., Galli, R., Belikov, S., Petrova, O. V., Sivkov, V. V., Vyalikh, D., Hunoldt, S., & Wörheide, G. (2013). Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris. Journal of Structural Biology, 183(3), 474-483. https://doi.org/10.1016/j.jsb.2013.06.015

Vancouver

Ehrlich H, Kaluzhnaya OV, Brunner E, Tsurkan MV, Ereskovsky A, Ilan M et al. Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris. Journal of Structural Biology. 2013 Sep;183(3):474-483. https://doi.org/10.1016/j.jsb.2013.06.015

Author

Ehrlich, Hermann ; Kaluzhnaya, Oksana V. ; Brunner, Eike ; Tsurkan, Mikhail V. ; Ereskovsky, Alexander ; Ilan, Micha ; Tabachnick, Konstantin R. ; Bazhenov, Vasilii V. ; Paasch, Silvia ; Kammer, Martin ; Born, René ; Stelling, Allison ; Galli, Roberta ; Belikov, Sergei ; Petrova, Olga V. ; Sivkov, Victor V. ; Vyalikh, Denis ; Hunoldt, Sebastian ; Wörheide, Gert. / Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris. In: Journal of Structural Biology. 2013 ; Vol. 183, No. 3. pp. 474-483.

BibTeX

@article{44af61f9e20443be959e00a36fc4ef73,
title = "Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris",
abstract = "This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.",
keywords = "Bioanalytics, Chitin, Chitin synthase, Skeleton, Sponges",
author = "Hermann Ehrlich and Kaluzhnaya, {Oksana V.} and Eike Brunner and Tsurkan, {Mikhail V.} and Alexander Ereskovsky and Micha Ilan and Tabachnick, {Konstantin R.} and Bazhenov, {Vasilii V.} and Silvia Paasch and Martin Kammer and Ren{\'e} Born and Allison Stelling and Roberta Galli and Sergei Belikov and Petrova, {Olga V.} and Sivkov, {Victor V.} and Denis Vyalikh and Sebastian Hunoldt and Gert W{\"o}rheide",
note = "Funding Information: Financial support by DFG (projects EH 394/1-1 and BR 1278/ 17-1) is gratefully acknowledged. Furthermore, this study was funded by Erasmus Mundus DAAD Program 2011, RFBR No. 11-04-00323-а, G-RISC (German-Russian Interdisciplinary Science Center) Grant 2012, the BMBF within the project CryPhysConcept (03EK3029A) and BHMZ Programme of Dr.-Erich-Kr{\"u}ger- Foundation (Germany). ",
year = "2013",
month = sep,
doi = "10.1016/j.jsb.2013.06.015",
language = "English",
volume = "183",
pages = "474--483",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

AU - Ehrlich, Hermann

AU - Kaluzhnaya, Oksana V.

AU - Brunner, Eike

AU - Tsurkan, Mikhail V.

AU - Ereskovsky, Alexander

AU - Ilan, Micha

AU - Tabachnick, Konstantin R.

AU - Bazhenov, Vasilii V.

AU - Paasch, Silvia

AU - Kammer, Martin

AU - Born, René

AU - Stelling, Allison

AU - Galli, Roberta

AU - Belikov, Sergei

AU - Petrova, Olga V.

AU - Sivkov, Victor V.

AU - Vyalikh, Denis

AU - Hunoldt, Sebastian

AU - Wörheide, Gert

N1 - Funding Information: Financial support by DFG (projects EH 394/1-1 and BR 1278/ 17-1) is gratefully acknowledged. Furthermore, this study was funded by Erasmus Mundus DAAD Program 2011, RFBR No. 11-04-00323-а, G-RISC (German-Russian Interdisciplinary Science Center) Grant 2012, the BMBF within the project CryPhysConcept (03EK3029A) and BHMZ Programme of Dr.-Erich-Krüger- Foundation (Germany).

PY - 2013/9

Y1 - 2013/9

N2 - This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.

AB - This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.

KW - Bioanalytics

KW - Chitin

KW - Chitin synthase

KW - Skeleton

KW - Sponges

UR - http://www.scopus.com/inward/record.url?scp=84883466136&partnerID=8YFLogxK

U2 - 10.1016/j.jsb.2013.06.015

DO - 10.1016/j.jsb.2013.06.015

M3 - Article

C2 - 23831449

AN - SCOPUS:84883466136

VL - 183

SP - 474

EP - 483

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -

ID: 97905067