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Human Artificial Chromosomes and Their Transfer to Target Cells. / Ponomartsev, S. V.; Sinenko, S. A.; Tomilin, A. N.

In: Acta Naturae, Vol. 14, No. 3, 29.10.2022, p. 35-45.

Research output: Contribution to journal › Article › peer-review

Harvard

Ponomartsev, SV, Sinenko, SA & Tomilin, AN 2022, 'Human Artificial Chromosomes and Their Transfer to Target Cells', Acta Naturae, vol. 14, no. 3, pp. 35-45. https://doi.org/10.32607/actanaturae.11670, https://doi.org/10.32607/actanaturae.11670

APA

Ponomartsev, S. V., Sinenko, S. A., & Tomilin, A. N. (2022). Human Artificial Chromosomes and Their Transfer to Target Cells. Acta Naturae, 14(3), 35-45. https://doi.org/10.32607/actanaturae.11670, https://doi.org/10.32607/actanaturae.11670

Vancouver

Ponomartsev SV, Sinenko SA, Tomilin AN. Human Artificial Chromosomes and Their Transfer to Target Cells. Acta Naturae. 2022 Oct 29;14(3):35-45. https://doi.org/10.32607/actanaturae.11670, https://doi.org/10.32607/actanaturae.11670

Author

Ponomartsev, S. V. ; Sinenko, S. A. ; Tomilin, A. N. / Human Artificial Chromosomes and Their Transfer to Target Cells. In: Acta Naturae. 2022 ; Vol. 14, No. 3. pp. 35-45.

BibTeX

@article{dd188e9bca2248a39e55598365a0b5e9,
title = "Human Artificial Chromosomes and Their Transfer to Target Cells",
abstract = "Human artificial chromosomes (HACs) have been developed as genetic vectors with the capacity to carry large transgenic constructs or entire gene loci. HACs represent either truncated native chromosomes or de novo synthesized genetic constructs. The important features of HACs are their ultra-high capacity and ability to self-maintain as independent genetic elements, without integrating into host chromosomes. In this review, we discuss the development and construction methods, structural and functional features, as well as the areas of application of the main HAC types. Also, we address one of the most technically challenging and time-consuming steps in this technology – the transfer of HACs from donor to recipient cells.",
keywords = "Human artificial chromosomes, Microcell-mediated chromosome transfer, Tetracycline operator, Transformation-associated recombination",
author = "Ponomartsev, {S. V.} and Sinenko, {S. A.} and Tomilin, {A. N.}",
note = "Publisher Copyright: {\textcopyright} 2022 National Research University Higher School of Economics. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.key:",
year = "2022",
month = oct,
day = "29",
doi = "10.32607/actanaturae.11670",
language = "English",
volume = "14",
pages = "35--45",
journal = "Acta Naturae",
issn = "2075-8251",
publisher = "Парк-медиа",
number = "3",

}

RIS

TY - JOUR

T1 - Human Artificial Chromosomes and Their Transfer to Target Cells

AU - Ponomartsev, S. V.

AU - Sinenko, S. A.

AU - Tomilin, A. N.

N1 - Publisher Copyright: © 2022 National Research University Higher School of Economics. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.key:

PY - 2022/10/29

Y1 - 2022/10/29

N2 - Human artificial chromosomes (HACs) have been developed as genetic vectors with the capacity to carry large transgenic constructs or entire gene loci. HACs represent either truncated native chromosomes or de novo synthesized genetic constructs. The important features of HACs are their ultra-high capacity and ability to self-maintain as independent genetic elements, without integrating into host chromosomes. In this review, we discuss the development and construction methods, structural and functional features, as well as the areas of application of the main HAC types. Also, we address one of the most technically challenging and time-consuming steps in this technology – the transfer of HACs from donor to recipient cells.

AB - Human artificial chromosomes (HACs) have been developed as genetic vectors with the capacity to carry large transgenic constructs or entire gene loci. HACs represent either truncated native chromosomes or de novo synthesized genetic constructs. The important features of HACs are their ultra-high capacity and ability to self-maintain as independent genetic elements, without integrating into host chromosomes. In this review, we discuss the development and construction methods, structural and functional features, as well as the areas of application of the main HAC types. Also, we address one of the most technically challenging and time-consuming steps in this technology – the transfer of HACs from donor to recipient cells.

KW - Human artificial chromosomes

KW - Microcell-mediated chromosome transfer

KW - Tetracycline operator

KW - Transformation-associated recombination

UR - http://www.scopus.com/inward/record.url?scp=85141781951&partnerID=8YFLogxK

U2 - 10.32607/actanaturae.11670

DO - 10.32607/actanaturae.11670

M3 - Article

VL - 14

SP - 35

EP - 45

JO - Acta Naturae

JF - Acta Naturae

SN - 2075-8251

IS - 3

ER -

ID: 100658403