TY - JOUR

T1 - HPLC-MS/MS determination of non-steroidal anti-inflammatory drugs in bovine milk based on simultaneous deep eutectic solvents formation and its solidification

AU - Shishov, Andrey

AU - Nechaeva, Daria

AU - Bulatov, Andrey

PY - 2019/11/1

Y1 - 2019/11/1

N2 - A simple, rapid, eco-friendly and effective microextraction procedure for separation and preconcentration of nonsteroidal anti-inflammatory drugs (NSAIDs) from bovine milk samples based on in situ deep eutectic solvents formation between menthol and NSAIDs and its solidification was developed. The procedure involved alkaline hydrolysis of sample matrix, mixing aqueous sample phase with molten menthol to obtain deep eutectic solvents and analytes extraction followed by organic phase (extract) separation based on its solidification. The conditions of in situ deep eutectic solvents formation were optimized and used for the HPLC-MS/MS determination of diclofenac, flurbiprofen, ketoprofen and mefenamic acid in bovine milk samples. The developed approach allowed to exclude matrixes effects and increase enrichment factors of target analytes. The LODs, calculated from the blank tests based on 3σ, were 0.01, 0.01, 0.01 and 0.03 μg kg− 1, for diclofenac, flurbiprofen, ketoprofen and mefenamic acid, respectively. The procedure was successfully applied to the analysis of real samples.

AB - A simple, rapid, eco-friendly and effective microextraction procedure for separation and preconcentration of nonsteroidal anti-inflammatory drugs (NSAIDs) from bovine milk samples based on in situ deep eutectic solvents formation between menthol and NSAIDs and its solidification was developed. The procedure involved alkaline hydrolysis of sample matrix, mixing aqueous sample phase with molten menthol to obtain deep eutectic solvents and analytes extraction followed by organic phase (extract) separation based on its solidification. The conditions of in situ deep eutectic solvents formation were optimized and used for the HPLC-MS/MS determination of diclofenac, flurbiprofen, ketoprofen and mefenamic acid in bovine milk samples. The developed approach allowed to exclude matrixes effects and increase enrichment factors of target analytes. The LODs, calculated from the blank tests based on 3σ, were 0.01, 0.01, 0.01 and 0.03 μg kg− 1, for diclofenac, flurbiprofen, ketoprofen and mefenamic acid, respectively. The procedure was successfully applied to the analysis of real samples.

KW - Bovine milk

KW - Deep eutectic mixture

KW - HPLC-MS/MS

KW - Menthol

KW - Microextraction

KW - Nonsteroidal anti-inflammatory drugs

KW - LIQUID-LIQUID MICROEXTRACTION

KW - NSAIDS

KW - CHROMATOGRAPHY

UR - http://www.scopus.com/inward/record.url?scp=85069448261&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2019.104080

DO - 10.1016/j.microc.2019.104080

M3 - Article

AN - SCOPUS:85069448261

VL - 150

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

M1 - 104080

ER -