TY - JOUR

T1 - High-resolution mapping of a/b compartments and topologically associated domains on giant lampbrush chromosomes

AU - Krasikova, A.

AU - Zlotina, A.

AU - Maslova, A.

AU - Starshova, P.

AU - Kulikova, T.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Progress in studies aimed at “deciphering” the spatial architecture of the genome is determined by the development of several key technologies: the chromatin conformation capture, ultra-high resolution optical microscopy and genomic locus imaging. At the same time, it remains unclear how the domains, determined by chromatin conformation capture technology, including topologically associated domains (TADs) and A/B compartments, are correlated with the chromatin domains detected at the cytological level. In the framework of this problem, a comprehensive study of the chromatin domains of giant lampbrush chromosomes characteristic of the growing oocytes in birds, amphibians and reptiles, seems appropriate. Methods: Here we aimed at comparing the chromomeres – the main structural unit of lampbrush chromosome axes – and topologically associated domains and A/B compartments in domestic chicken (Gallus gallus domesticus), whose genome was the first among the deciphered avian genomes. In addition, earlier, using the full-genome Hi-C method a number of hierarchical structural domains, such as A and B compartments and TADs, were identified in chicken embryonic fibroblasts. Results: The results obtained allowed us to verify the hypothesis of the correspondence between the globular-loop chromatin domains of the interphase nucleus and the chromomere-loop complexes of lampbrush chromosomes, as well as to shed light on the nature of the lampbrush chromosome chromomeres.

AB - Progress in studies aimed at “deciphering” the spatial architecture of the genome is determined by the development of several key technologies: the chromatin conformation capture, ultra-high resolution optical microscopy and genomic locus imaging. At the same time, it remains unclear how the domains, determined by chromatin conformation capture technology, including topologically associated domains (TADs) and A/B compartments, are correlated with the chromatin domains detected at the cytological level. In the framework of this problem, a comprehensive study of the chromatin domains of giant lampbrush chromosomes characteristic of the growing oocytes in birds, amphibians and reptiles, seems appropriate. Methods: Here we aimed at comparing the chromomeres – the main structural unit of lampbrush chromosome axes – and topologically associated domains and A/B compartments in domestic chicken (Gallus gallus domesticus), whose genome was the first among the deciphered avian genomes. In addition, earlier, using the full-genome Hi-C method a number of hierarchical structural domains, such as A and B compartments and TADs, were identified in chicken embryonic fibroblasts. Results: The results obtained allowed us to verify the hypothesis of the correspondence between the globular-loop chromatin domains of the interphase nucleus and the chromomere-loop complexes of lampbrush chromosomes, as well as to shed light on the nature of the lampbrush chromosome chromomeres.

KW - animal experiment

KW - Article

KW - chicken

KW - Chromatin

KW - chromosome

KW - controlled study

KW - fibroblast

KW - interphase

KW - nonhuman

KW - writing

UR - http://www.scopus.com/inward/record.url?scp=85073274128&partnerID=8YFLogxK

U2 - 10.7124/bc.0009AF

DO - 10.7124/bc.0009AF

M3 - Article

AN - SCOPUS:85073274128

VL - 35

SP - 175

JO - Biopolymers and Cell

JF - Biopolymers and Cell

SN - 0233-7657

IS - 3

ER -