Research output: Contribution to journal › Article › peer-review
HETEROLOGOUS INTERFERONS SYNTHESIS IN YEAST PICHIA PASTORIS. / Padkina, M. V.; Parfenova, L. V.; Gradoboeva, A. E.; Самбук, Елена Викторовна.
In: Applied Biochemistry and Microbiology, Vol. 46, No. 4, 2010.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - HETEROLOGOUS INTERFERONS SYNTHESIS IN YEAST PICHIA PASTORIS
AU - Padkina, M. V.
AU - Parfenova, L. V.
AU - Gradoboeva, A. E.
AU - Самбук, Елена Викторовна
PY - 2010
Y1 - 2010
N2 - The HuIFNA16, HuIFNB1, and BoIFNG genes encoding human alpha 16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed. There was no effect of high level of heterologous protein synthesis on the yeast P. pastoris cell growth, unlike yeast Saccharomyces cerevisiae. The considerable part of the heterologous interferons was detected in the yeast P. pastoris soluble protein fraction but not in the "inclusion bodies." The treatment of human beta-interferon with endoglycosidase H showed that protein was expressed in glycosylated and unglycosylated forms. On the strength of these data, the hypothesis was suggested that the more effective heterologous gene expression in yeast P. pastoris and enhanced resistance of the methylotrophic yeast to negative effects of recombinant proteins was due to the special features of its metabolism.
AB - The HuIFNA16, HuIFNB1, and BoIFNG genes encoding human alpha 16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed. There was no effect of high level of heterologous protein synthesis on the yeast P. pastoris cell growth, unlike yeast Saccharomyces cerevisiae. The considerable part of the heterologous interferons was detected in the yeast P. pastoris soluble protein fraction but not in the "inclusion bodies." The treatment of human beta-interferon with endoglycosidase H showed that protein was expressed in glycosylated and unglycosylated forms. On the strength of these data, the hypothesis was suggested that the more effective heterologous gene expression in yeast P. pastoris and enhanced resistance of the methylotrophic yeast to negative effects of recombinant proteins was due to the special features of its metabolism.
U2 - 10.1134/S0003683810040083
DO - 10.1134/S0003683810040083
M3 - Article
VL - 46
JO - Applied Biochemistry and Microbiology
JF - Applied Biochemistry and Microbiology
SN - 0003-6838
IS - 4
ER -
ID: 8183025