Research output: Contribution to journal › Article › peer-review
Hadmolekuliarnaia organizatsiia aminotransferaz i degidrogenaz alpha- ketokislot mitochondriǐ golovnogo mozga krys. / Rozanov, V. A.; Fan Ban T'i, Ban T'i; Gerasimiak, G. R.; Liubarev, A. E.; Kurganov, B. I.; Rozanov, A. I.
In: Ukrainskii biokhimicheskii zhurnal, Vol. 63, No. 2, 01.03.1991, p. 66-71.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Hadmolekuliarnaia organizatsiia aminotransferaz i degidrogenaz alpha- ketokislot mitochondriǐ golovnogo mozga krys.
AU - Rozanov, V. A.
AU - Fan Ban T'i, Ban T'i
AU - Gerasimiak, G. R.
AU - Liubarev, A. E.
AU - Kurganov, B. I.
AU - Rozanov, A. I.
PY - 1991/3/1
Y1 - 1991/3/1
N2 - A fraction of coarse mitochondria from the rat brain was deposited after short-term effect of supersound to obtain "metabolones". Activity of dehydrogenases of alpha-ketoacids, succinate dehydrogenase, aspartate-, alanine- and GABA-alpha-ketoglutarate amino-transferases has been determined in the supernatant liquid and in "metabolones". It is shown that dehydrogenase activity is mainly (93-100%) localized in "metabolones", while the level of aminotransferase activity in the latter is lower (72-94%). Nonproportionally high activity of aminotransferases in the supernatant liquid is found to considerably suprpass a decrease in activity of these enzymes in "metabolones" against a background of extremely scanty losses of protein (within 5%) induced by the supersound effect. A hypothetic model of a "metabolone" containing the enzymes of the Krebbs cycle and GABA-shunt is suggested.
AB - A fraction of coarse mitochondria from the rat brain was deposited after short-term effect of supersound to obtain "metabolones". Activity of dehydrogenases of alpha-ketoacids, succinate dehydrogenase, aspartate-, alanine- and GABA-alpha-ketoglutarate amino-transferases has been determined in the supernatant liquid and in "metabolones". It is shown that dehydrogenase activity is mainly (93-100%) localized in "metabolones", while the level of aminotransferase activity in the latter is lower (72-94%). Nonproportionally high activity of aminotransferases in the supernatant liquid is found to considerably suprpass a decrease in activity of these enzymes in "metabolones" against a background of extremely scanty losses of protein (within 5%) induced by the supersound effect. A hypothetic model of a "metabolone" containing the enzymes of the Krebbs cycle and GABA-shunt is suggested.
UR - http://www.scopus.com/inward/record.url?scp=0026128048&partnerID=8YFLogxK
M3 - статья
C2 - 1882466
AN - SCOPUS:0026128048
VL - 63
SP - 66
EP - 71
JO - Ukrainian biochemical journal
JF - Ukrainian biochemical journal
SN - 2409-4943
IS - 2
ER -
ID: 42769663