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Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism. / Pchelin, Ivan M.; Mochalov, Yuri V.; Azarov, Daniil V.; Romanyuk, Sofya A.; Chilina, Galina A.; Vybornova, Irina V.; Bogdanova, Tatiyana V.; Zlatogursky, Vasily V.; Apalko, Svetlana V.; Vasilyeva, Natalia V.; Taraskina, Anastasia E.

In: Mycoses, Vol. 63, No. 11, 01.11.2020, p. 1244-1254.

Research output: Contribution to journalArticlepeer-review

Harvard

Pchelin, IM, Mochalov, YV, Azarov, DV, Romanyuk, SA, Chilina, GA, Vybornova, IV, Bogdanova, TV, Zlatogursky, VV, Apalko, SV, Vasilyeva, NV & Taraskina, AE 2020, 'Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism', Mycoses, vol. 63, no. 11, pp. 1244-1254. https://doi.org/10.1111/myc.13162

APA

Pchelin, I. M., Mochalov, Y. V., Azarov, D. V., Romanyuk, S. A., Chilina, G. A., Vybornova, I. V., Bogdanova, T. V., Zlatogursky, V. V., Apalko, S. V., Vasilyeva, N. V., & Taraskina, A. E. (2020). Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism. Mycoses, 63(11), 1244-1254. https://doi.org/10.1111/myc.13162

Vancouver

Pchelin IM, Mochalov YV, Azarov DV, Romanyuk SA, Chilina GA, Vybornova IV et al. Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism. Mycoses. 2020 Nov 1;63(11):1244-1254. https://doi.org/10.1111/myc.13162

Author

Pchelin, Ivan M. ; Mochalov, Yuri V. ; Azarov, Daniil V. ; Romanyuk, Sofya A. ; Chilina, Galina A. ; Vybornova, Irina V. ; Bogdanova, Tatiyana V. ; Zlatogursky, Vasily V. ; Apalko, Svetlana V. ; Vasilyeva, Natalia V. ; Taraskina, Anastasia E. / Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism. In: Mycoses. 2020 ; Vol. 63, No. 11. pp. 1244-1254.

BibTeX

@article{f83aa52b5b8b4407a69d5bd9650d7e66,
title = "Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism",
abstract = "Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5{\textquoteright}-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.",
keywords = "dermatophyte, microsatellites, molecular strain typing, population structure, Trichophyton rubrum, whole-genome sequencing",
author = "Pchelin, {Ivan M.} and Mochalov, {Yuri V.} and Azarov, {Daniil V.} and Romanyuk, {Sofya A.} and Chilina, {Galina A.} and Vybornova, {Irina V.} and Bogdanova, {Tatiyana V.} and Zlatogursky, {Vasily V.} and Apalko, {Svetlana V.} and Vasilyeva, {Natalia V.} and Taraskina, {Anastasia E.}",
note = "Funding Information: This work was supported by Russian Foundation for Basic Research [grant number 18-34-00153]. The authors would like to thank the CBS-KNAW culture collection of Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands, for providing the strain CBS 392.58. We also appreciate valuable comments from Ad?la ?mokov? and Ali Rezaei-Matehkolaei. Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = nov,
day = "1",
doi = "10.1111/myc.13162",
language = "English",
volume = "63",
pages = "1244--1254",
journal = "Mycoses",
issn = "0933-7407",
publisher = "Wiley-Blackwell",
number = "11",

}

RIS

TY - JOUR

T1 - Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism

AU - Pchelin, Ivan M.

AU - Mochalov, Yuri V.

AU - Azarov, Daniil V.

AU - Romanyuk, Sofya A.

AU - Chilina, Galina A.

AU - Vybornova, Irina V.

AU - Bogdanova, Tatiyana V.

AU - Zlatogursky, Vasily V.

AU - Apalko, Svetlana V.

AU - Vasilyeva, Natalia V.

AU - Taraskina, Anastasia E.

N1 - Funding Information: This work was supported by Russian Foundation for Basic Research [grant number 18-34-00153]. The authors would like to thank the CBS-KNAW culture collection of Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands, for providing the strain CBS 392.58. We also appreciate valuable comments from Ad?la ?mokov? and Ali Rezaei-Matehkolaei. Publisher Copyright: © 2020 Wiley-VCH GmbH Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/1

Y1 - 2020/11/1

N2 - Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5’-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.

AB - Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5’-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.

KW - dermatophyte

KW - microsatellites

KW - molecular strain typing

KW - population structure

KW - Trichophyton rubrum

KW - whole-genome sequencing

UR - http://www.scopus.com/inward/record.url?scp=85090448246&partnerID=8YFLogxK

U2 - 10.1111/myc.13162

DO - 10.1111/myc.13162

M3 - Article

C2 - 32785975

AN - SCOPUS:85090448246

VL - 63

SP - 1244

EP - 1254

JO - Mycoses

JF - Mycoses

SN - 0933-7407

IS - 11

ER -

ID: 71412653