Research output: Contribution to journal › Article › peer-review
Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism. / Pchelin, Ivan M.; Mochalov, Yuri V.; Azarov, Daniil V.; Romanyuk, Sofya A.; Chilina, Galina A.; Vybornova, Irina V.; Bogdanova, Tatiyana V.; Zlatogursky, Vasily V.; Apalko, Svetlana V.; Vasilyeva, Natalia V.; Taraskina, Anastasia E.
In: Mycoses, Vol. 63, No. 11, 01.11.2020, p. 1244-1254.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism
AU - Pchelin, Ivan M.
AU - Mochalov, Yuri V.
AU - Azarov, Daniil V.
AU - Romanyuk, Sofya A.
AU - Chilina, Galina A.
AU - Vybornova, Irina V.
AU - Bogdanova, Tatiyana V.
AU - Zlatogursky, Vasily V.
AU - Apalko, Svetlana V.
AU - Vasilyeva, Natalia V.
AU - Taraskina, Anastasia E.
N1 - Funding Information: This work was supported by Russian Foundation for Basic Research [grant number 18-34-00153]. The authors would like to thank the CBS-KNAW culture collection of Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands, for providing the strain CBS 392.58. We also appreciate valuable comments from Ad?la ?mokov? and Ali Rezaei-Matehkolaei. Publisher Copyright: © 2020 Wiley-VCH GmbH Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5’-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.
AB - Background: The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T rubrum sensu stricto and T violaceum, as well as other less well-established or debatable taxa like T soudanense, T kuryangei and T megninii. Our previous study provided limited evidence in favour of the existence of two genetic lineages in the Russian T rubrum sensu stricto population. Objectives: We aimed to study the genetic structure of the Russian population of T rubrum and to identify factors shaping this structure. Methods: We analysed the polymorphism of 12 simple sequence repeat (SSR or microsatellite) markers and single nucleotide polymorphism in the TERG_02941 protein-coding gene in 70 T rubrum isolates and performed a phylogenomic reconstruction. Results: All three types of data provided conclusive evidence that the population consists of two genetic lineages. Clustering, performed by means of microsatellite length polymorphism analysis, was strongly dependent on the number of nucleotide repeats in the 5’-area of the fructose-1,6-bisphosphate aldolase gene. Analysis of molecular variance (AMOVA) on the basis of SSR typing data indicated that 22%-48% of the variability was among groups within T rubrum. There was no clear connection of population structure with types of infection, places of geographic origin, aldolase gene expression or urease activity. Conclusion: Our results suggest that the Russian population of T rubrum consists of two cosmopolitan genetic lineages.
KW - dermatophyte
KW - microsatellites
KW - molecular strain typing
KW - population structure
KW - Trichophyton rubrum
KW - whole-genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85090448246&partnerID=8YFLogxK
U2 - 10.1111/myc.13162
DO - 10.1111/myc.13162
M3 - Article
C2 - 32785975
AN - SCOPUS:85090448246
VL - 63
SP - 1244
EP - 1254
JO - Mycoses
JF - Mycoses
SN - 0933-7407
IS - 11
ER -
ID: 71412653