Genetic and morphological variation of metacercariae of Microphallus piriformes (Trematoda, Microphallidae): effects of paraxenia and geographic location

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Genetic and morphological variation of metacercariae of Microphallus piriformes (Trematoda, Microphallidae): effects of paraxenia and geographic location. / Repkin, Egor A.; Maltseva, Arina L.; Varfolomeeva, Marina A.; Aianka, Roman V.; Mikhailova, Natalia A.; Granovitch, Andrei I.

In: International Journal for Parasitology: Parasites and Wildlife, Vol. 11, 235-245, 04.2020, p. 235-245.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{b1e453f3d46148b594d84ea999f55f1f,

title = "Genetic and morphological variation of metacercariae of Microphallus piriformes (Trematoda, Microphallidae): effects of paraxenia and geographic location",

abstract = "Host organism offers an environment for a parasite, and this environment is heterogenous within the host, variable among individual as well as between the hosts, and changing during the host's lifetime. This heterogeneity may act as a prerequisite for parasite species divergence. Intraspecific variability related to a certain type of heterogeneity may indicate an initial stage of speciation, and thus poses an evolutionary importance. Here we analyzed genetic and morphologic variation of trematode metacercariae of Microphallus piriformes (Trematoda, Microphallidae). Genetic variability of trematodes was assessed from sequences of cytochrome c oxidase subunit 1 (COI) and internal transcribed spacer region (ITS-1). Morphological variation of metacercarial body shape was for the first time analyzed using geometric morphometrics. Parasites from the White Sea and the Barents Sea coasts demonstrated partial genetic divergence (according to COI sequence analysis) and had significantly different body shape. Neither genetic nor morphological variation of metacercariae was related to intermediate host species. We discuss possible causes of the observed genetic divergence of parasite populations in different geographic regions.",

keywords = "Developmental stability, Geometric morphometrics, Microphallus piriformes, Molecular markers, Paraxenia, Trematoda",

author = "Repkin, {Egor A.} and Maltseva, {Arina L.} and Varfolomeeva, {Marina A.} and Aianka, {Roman V.} and Mikhailova, {Natalia A.} and Granovitch, {Andrei I.}",

note = "Funding Information: This research was funded by the Russian Foundation for Basic Research grants number 19-04-00392 ; the purchase of basic laboratory equipment was financed by the St. Petersburg State University grant number 0.40.491.2017 . The opportunities for DNA purification, amplification and sequencing were provided by the Resource Center “Molecular and Cell Technologies”; data analysis was performed in the Computing Centre of the St. Petersburg State University; experimental animals were maintained in the Resource Center “Observatory of environmental safety” of St. Petersburg State University. Sampling in the White Sea coast was logistically based on the Educational and research station “Belomorskaia” of the St. Petersburg State University. We thank Eugeniy L. Yakovis for thorough proofreading and language editing.",

year = "2020",

month = apr,

doi = "10.1016/j.ijppaw.2020.02.004",

language = "English",

volume = "11",

pages = "235--245",

journal = "International Journal for Parasitology: Parasites and Wildlife",

issn = "2213-2244",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Genetic and morphological variation of metacercariae of Microphallus piriformes (Trematoda, Microphallidae): effects of paraxenia and geographic location

AU - Repkin, Egor A.

AU - Maltseva, Arina L.

AU - Varfolomeeva, Marina A.

AU - Aianka, Roman V.

AU - Mikhailova, Natalia A.

AU - Granovitch, Andrei I.

N1 - Funding Information: This research was funded by the Russian Foundation for Basic Research grants number 19-04-00392 ; the purchase of basic laboratory equipment was financed by the St. Petersburg State University grant number 0.40.491.2017 . The opportunities for DNA purification, amplification and sequencing were provided by the Resource Center “Molecular and Cell Technologies”; data analysis was performed in the Computing Centre of the St. Petersburg State University; experimental animals were maintained in the Resource Center “Observatory of environmental safety” of St. Petersburg State University. Sampling in the White Sea coast was logistically based on the Educational and research station “Belomorskaia” of the St. Petersburg State University. We thank Eugeniy L. Yakovis for thorough proofreading and language editing.

PY - 2020/4

Y1 - 2020/4

N2 - Host organism offers an environment for a parasite, and this environment is heterogenous within the host, variable among individual as well as between the hosts, and changing during the host's lifetime. This heterogeneity may act as a prerequisite for parasite species divergence. Intraspecific variability related to a certain type of heterogeneity may indicate an initial stage of speciation, and thus poses an evolutionary importance. Here we analyzed genetic and morphologic variation of trematode metacercariae of Microphallus piriformes (Trematoda, Microphallidae). Genetic variability of trematodes was assessed from sequences of cytochrome c oxidase subunit 1 (COI) and internal transcribed spacer region (ITS-1). Morphological variation of metacercarial body shape was for the first time analyzed using geometric morphometrics. Parasites from the White Sea and the Barents Sea coasts demonstrated partial genetic divergence (according to COI sequence analysis) and had significantly different body shape. Neither genetic nor morphological variation of metacercariae was related to intermediate host species. We discuss possible causes of the observed genetic divergence of parasite populations in different geographic regions.

AB - Host organism offers an environment for a parasite, and this environment is heterogenous within the host, variable among individual as well as between the hosts, and changing during the host's lifetime. This heterogeneity may act as a prerequisite for parasite species divergence. Intraspecific variability related to a certain type of heterogeneity may indicate an initial stage of speciation, and thus poses an evolutionary importance. Here we analyzed genetic and morphologic variation of trematode metacercariae of Microphallus piriformes (Trematoda, Microphallidae). Genetic variability of trematodes was assessed from sequences of cytochrome c oxidase subunit 1 (COI) and internal transcribed spacer region (ITS-1). Morphological variation of metacercarial body shape was for the first time analyzed using geometric morphometrics. Parasites from the White Sea and the Barents Sea coasts demonstrated partial genetic divergence (according to COI sequence analysis) and had significantly different body shape. Neither genetic nor morphological variation of metacercariae was related to intermediate host species. We discuss possible causes of the observed genetic divergence of parasite populations in different geographic regions.

KW - Developmental stability

KW - Geometric morphometrics

KW - Microphallus piriformes

KW - Molecular markers

KW - Paraxenia

KW - Trematoda

UR - http://www.scopus.com/inward/record.url?scp=85081647267&partnerID=8YFLogxK

U2 - 10.1016/j.ijppaw.2020.02.004

DO - 10.1016/j.ijppaw.2020.02.004

M3 - Article

VL - 11

SP - 235

EP - 245

JO - International Journal for Parasitology: Parasites and Wildlife

JF - International Journal for Parasitology: Parasites and Wildlife

SN - 2213-2244

M1 - 235-245

ER -

ID: 51230670