Genetic Analysis of Revertants of Chlorophyll b-Deficient Mutants of Chlamydomonas reinhardtii

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Genetic Analysis of Revertants of Chlorophyll b-Deficient Mutants of Chlamydomonas reinhardtii. / Nikulina, K. V.; Chekunova, E. M.; Rüdiger, W.; Chunaev, A. S.

In: Russian Journal of Genetics, Vol. 33, No. 5, 05.1997, p. 474-479.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{02c3ac053a9f4489bd792ed71c44cbd4,

title = "Genetic Analysis of Revertants of Chlorophyll b-Deficient Mutants of Chlamydomonas reinhardtii",

abstract = "From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnI gene, 15 different revertants were obtained and analyzed. In 13 revertants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revertants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revertants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnI gene. By means of tetrad analysis, five strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnI gene were isolated. No allelic specificity of suppression was found.",

author = "Nikulina, {K. V.} and Chekunova, {E. M.} and W. R{\"u}diger and Chunaev, {A. S.}",

year = "1997",

month = may,

language = "English",

volume = "33",

pages = "474--479",

journal = "Russian Journal of Genetics",

issn = "1022-7954",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "5",

}

RIS

TY - JOUR

T1 - Genetic Analysis of Revertants of Chlorophyll b-Deficient Mutants of Chlamydomonas reinhardtii

AU - Nikulina, K. V.

AU - Chekunova, E. M.

AU - Rüdiger, W.

AU - Chunaev, A. S.

PY - 1997/5

Y1 - 1997/5

N2 - From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnI gene, 15 different revertants were obtained and analyzed. In 13 revertants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revertants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revertants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnI gene. By means of tetrad analysis, five strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnI gene were isolated. No allelic specificity of suppression was found.

AB - From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnI gene, 15 different revertants were obtained and analyzed. In 13 revertants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revertants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revertants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnI gene. By means of tetrad analysis, five strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnI gene were isolated. No allelic specificity of suppression was found.

UR - http://www.scopus.com/inward/record.url?scp=1842764848&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:1842764848

VL - 33

SP - 474

EP - 479

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 5

ER -

ID: 76658379