Genetic analysis of revertants of chlorophyll - Deficient mutants of chlamydomonas reinhardtii

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Genetic analysis of revertants of chlorophyll - Deficient mutants of chlamydomonas reinhardtii. / Nikulina, K. V.; Chekunova, E. M.; Rüdiger, W.; Chunaev, A. S.

In: Genetika, Vol. 33, No. 5, 1997, p. 577-582.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{55d72226b2574234b1154c8f468f565e,

title = "Genetic analysis of revertants of chlorophyll - Deficient mutants of chlamydomonas reinhardtii",

abstract = "From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnl gene, 15 different revenants were obtained and analyzed. In 13 revenants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revenants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revenants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnl gene. By means of tetrad analysis, four strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnl gene were isolated. No allelic specificity of suppression was: found.",

author = "Nikulina, {K. V.} and Chekunova, {E. M.} and W. R{\"u}diger and Chunaev, {A. S.}",

year = "1997",

language = "English",

volume = "33",

pages = "577--582",

journal = "ГЕНЕТИКА",

issn = "0016-6758",

publisher = "Международная книга",

number = "5",

}

RIS

TY - JOUR

T1 - Genetic analysis of revertants of chlorophyll - Deficient mutants of chlamydomonas reinhardtii

AU - Nikulina, K. V.

AU - Chekunova, E. M.

AU - Rüdiger, W.

AU - Chunaev, A. S.

PY - 1997

Y1 - 1997

N2 - From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnl gene, 15 different revenants were obtained and analyzed. In 13 revenants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revenants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revenants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnl gene. By means of tetrad analysis, four strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnl gene were isolated. No allelic specificity of suppression was: found.

AB - From Chlamydomonas reinhardtii chlorophyll b- and neoxanthin-depleted mutants in the cbnl gene, 15 different revenants were obtained and analyzed. In 13 revenants, the complete recovery of chlorophyll b and neoxanthin biosyntheses to the level of the wild-type strain was shown. In two revenants, the biosynthesis recovery of the studied pigments was incomplete (10 and 30% of wild-type chlorophyll b biosynthesis). Single mutations in nuclear suppressor genes were shown by hybridological analysis to be responsible for the recovery of chlorophyll b and neoxanthins accumulation in most revenants. Revertants were distinguished by the degree of linkage between the suppressor and original mutation in the cbnl gene. By means of tetrad analysis, four strains carrying various mutant alleles of suppressor genes in the background of a normal allele of the cbnl gene were isolated. No allelic specificity of suppression was: found.

UR - http://www.scopus.com/inward/record.url?scp=33746164843&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33746164843

VL - 33

SP - 577

EP - 582

JO - ГЕНЕТИКА

JF - ГЕНЕТИКА

SN - 0016-6758

IS - 5

ER -

ID: 76658252