Generation of Hydrogen Peroxide and Transcriptional Regulation of Antioxidant Enzyme Expression in Chlamydomonas reinhardtii under Hypothermia

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Generation of Hydrogen Peroxide and Transcriptional Regulation of Antioxidant Enzyme Expression in Chlamydomonas reinhardtii under Hypothermia. / Zalutskaya, Zh M.; Skryabina, U. S.; Ermilova, E. V.

In: Russian Journal of Plant Physiology, Vol. 66, No. 2, 01.03.2019, p. 223-230.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{39c3ac33db5d49f4bf5b321a4a87f752,

title = "Generation of Hydrogen Peroxide and Transcriptional Regulation of Antioxidant Enzyme Expression in Chlamydomonas reinhardtii under Hypothermia",

abstract = "Abstract: In the present work, generation of hydrogen peroxide in Chlamydomonas reinhardtii Dang. cells under hypothermia was demonstrated. Analysis in these cells of genes' transcription encoding the enzymes involved in detoxification of H2O2 in them, such as ascorbate peroxidases (APX1, APX2 and APX3), catalases (CAT1 and CAT2), and glutathione peroxidases (GPX1, GPX3, GPX4 and GPX5), showed that action of low temperature (7°C) leads to lowering transcription of genes encoding catalases and ascorbate peroxidases. At the same time, induction of three GPX genes, GPX1, GPX3, and GPX5, was found under these conditions. Maximal (50 times) elevation in transcription levels was determined for the GPX3 gene. As a result, the gene encoding glutathione peroxidase 3 is suggested as a potential marker for C. reinhardtii{\textquoteright}s response to cold treatment. In addition, it was shown that hydrogen peroxide is involved in the regulation of the induction of the GPX3 gene under the action of low temperature.",

keywords = "antioxidant systems, Chlamydomonas reinhardtii, glutathione peroxidases, hydrogen peroxide, hypothermia, quantitative PCR, CATALASE, SIGNAL, OXIDATIVE STRESS, HOMOLOGOUS GENE, PEROXIREDOXIN, LIGHT, SENSORS, EXPOSURE, INSIGHTS",

author = "Zalutskaya, {Zh M.} and Skryabina, {U. S.} and Ermilova, {E. V.}",

year = "2019",

month = mar,

day = "1",

doi = "10.1134/S1021443719020171",

language = "English",

volume = "66",

pages = "223--230",

journal = "Russian Journal of Plant Physiology",

issn = "1021-4437",

publisher = "Pleiades Publishing",

number = "2",

}

RIS

TY - JOUR

T1 - Generation of Hydrogen Peroxide and Transcriptional Regulation of Antioxidant Enzyme Expression in Chlamydomonas reinhardtii under Hypothermia

AU - Zalutskaya, Zh M.

AU - Skryabina, U. S.

AU - Ermilova, E. V.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Abstract: In the present work, generation of hydrogen peroxide in Chlamydomonas reinhardtii Dang. cells under hypothermia was demonstrated. Analysis in these cells of genes' transcription encoding the enzymes involved in detoxification of H2O2 in them, such as ascorbate peroxidases (APX1, APX2 and APX3), catalases (CAT1 and CAT2), and glutathione peroxidases (GPX1, GPX3, GPX4 and GPX5), showed that action of low temperature (7°C) leads to lowering transcription of genes encoding catalases and ascorbate peroxidases. At the same time, induction of three GPX genes, GPX1, GPX3, and GPX5, was found under these conditions. Maximal (50 times) elevation in transcription levels was determined for the GPX3 gene. As a result, the gene encoding glutathione peroxidase 3 is suggested as a potential marker for C. reinhardtii’s response to cold treatment. In addition, it was shown that hydrogen peroxide is involved in the regulation of the induction of the GPX3 gene under the action of low temperature.

AB - Abstract: In the present work, generation of hydrogen peroxide in Chlamydomonas reinhardtii Dang. cells under hypothermia was demonstrated. Analysis in these cells of genes' transcription encoding the enzymes involved in detoxification of H2O2 in them, such as ascorbate peroxidases (APX1, APX2 and APX3), catalases (CAT1 and CAT2), and glutathione peroxidases (GPX1, GPX3, GPX4 and GPX5), showed that action of low temperature (7°C) leads to lowering transcription of genes encoding catalases and ascorbate peroxidases. At the same time, induction of three GPX genes, GPX1, GPX3, and GPX5, was found under these conditions. Maximal (50 times) elevation in transcription levels was determined for the GPX3 gene. As a result, the gene encoding glutathione peroxidase 3 is suggested as a potential marker for C. reinhardtii’s response to cold treatment. In addition, it was shown that hydrogen peroxide is involved in the regulation of the induction of the GPX3 gene under the action of low temperature.

KW - antioxidant systems

KW - Chlamydomonas reinhardtii

KW - glutathione peroxidases

KW - hydrogen peroxide

KW - hypothermia

KW - quantitative PCR

KW - CATALASE

KW - SIGNAL

KW - OXIDATIVE STRESS

KW - HOMOLOGOUS GENE

KW - PEROXIREDOXIN

KW - LIGHT

KW - SENSORS

KW - EXPOSURE

KW - INSIGHTS

UR - http://www.scopus.com/inward/record.url?scp=85068556375&partnerID=8YFLogxK

U2 - 10.1134/S1021443719020171

DO - 10.1134/S1021443719020171

M3 - Article

AN - SCOPUS:85068556375

VL - 66

SP - 223

EP - 230

JO - Russian Journal of Plant Physiology

JF - Russian Journal of Plant Physiology

SN - 1021-4437

IS - 2

ER -

ID: 45231488