Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes

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Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes : A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia. / Wu, Cheng Ham; Kisel, Kristina S.; Thangavel, Muthu Kumar; Chen, Yi Ting; Chang, Kai Hsin; Tsai, Ming Rung; Chu, Chia Yu; Shen, Yu Fang; Wu, Pei Chun; Zhang, Zhiming; Liu, Tzu Ming; Jänis, Janne; Grachova, Elena V.; Shakirova, Julia R.; Tunik, Sergey P.; Koshevoy, Igor O.; Chou, Pi Tai.

In: Advanced Science, Vol. 8, No. 20, 2102788, 20.10.2021.

Research output: Contribution to journal › Article › peer-review

Author

Wu, Cheng Ham ; Kisel, Kristina S. ; Thangavel, Muthu Kumar ; Chen, Yi Ting ; Chang, Kai Hsin ; Tsai, Ming Rung ; Chu, Chia Yu ; Shen, Yu Fang ; Wu, Pei Chun ; Zhang, Zhiming ; Liu, Tzu Ming ; Jänis, Janne ; Grachova, Elena V. ; Shakirova, Julia R. ; Tunik, Sergey P. ; Koshevoy, Igor O. ; Chou, Pi Tai. / Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes : A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia. In: Advanced Science. 2021 ; Vol. 8, No. 20.

BibTeX

@article{be4cbe7e250444589bb61622bd061af8,

title = "Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes: A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia",

abstract = "The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI-diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.",

keywords = "phosphorescence lifetime imaging microscopy, phosphorescent oxygen sensors, Re diimine carbonyl complexes, tissue ischemia, tumor hypoxia, two-photon phosphorescence, DESIGN, RHENIUM(I) COMPLEXES, Re-I diimine carbonyl complexes, MITOCHONDRIA, IMAGING MICROSCOPY, LUMINESCENCE, ABSORPTION CROSS-SECTIONS, PHOTOPHYSICS, EMISSION, METAL-COMPLEXES, LIVING CELLS, ReI diimine carbonyl complexes",

author = "Wu, {Cheng Ham} and Kisel, {Kristina S.} and Thangavel, {Muthu Kumar} and Chen, {Yi Ting} and Chang, {Kai Hsin} and Tsai, {Ming Rung} and Chu, {Chia Yu} and Shen, {Yu Fang} and Wu, {Pei Chun} and Zhiming Zhang and Liu, {Tzu Ming} and Janne J{\"a}nis and Grachova, {Elena V.} and Shakirova, {Julia R.} and Tunik, {Sergey P.} and Koshevoy, {Igor O.} and Chou, {Pi Tai}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Advanced Science published by Wiley-VCH GmbH",

year = "2021",

month = oct,

day = "20",

doi = "10.1002/advs.202102788",

language = "English",

volume = "8",

journal = "Advanced Science",

issn = "2198-3844",

publisher = "Wiley-Blackwell",

number = "20",

}

RIS

TY - JOUR

T1 - Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes

T2 - A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia

AU - Wu, Cheng Ham

AU - Kisel, Kristina S.

AU - Thangavel, Muthu Kumar

AU - Chen, Yi Ting

AU - Chang, Kai Hsin

AU - Tsai, Ming Rung

AU - Chu, Chia Yu

AU - Shen, Yu Fang

AU - Wu, Pei Chun

AU - Zhang, Zhiming

AU - Liu, Tzu Ming

AU - Jänis, Janne

AU - Grachova, Elena V.

AU - Shakirova, Julia R.

AU - Tunik, Sergey P.

AU - Koshevoy, Igor O.

AU - Chou, Pi Tai

PY - 2021/10/20

Y1 - 2021/10/20

N2 - The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI-diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.

AB - The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI-diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.

KW - phosphorescence lifetime imaging microscopy

KW - phosphorescent oxygen sensors

KW - Re diimine carbonyl complexes

KW - tissue ischemia

KW - tumor hypoxia

KW - two-photon phosphorescence

KW - DESIGN

KW - RHENIUM(I) COMPLEXES

KW - Re-I diimine carbonyl complexes

KW - MITOCHONDRIA

KW - IMAGING MICROSCOPY

KW - LUMINESCENCE

KW - ABSORPTION CROSS-SECTIONS

KW - PHOTOPHYSICS

KW - EMISSION

KW - METAL-COMPLEXES

KW - LIVING CELLS

KW - ReI diimine carbonyl complexes

UR - http://www.scopus.com/inward/record.url?scp=85113135189&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/2406aca4-9c65-3a96-8c16-8e7204fb04fa/

U2 - 10.1002/advs.202102788

DO - 10.1002/advs.202102788

M3 - Article

C2 - 34414696

AN - SCOPUS:85113135189

VL - 8

JO - Advanced Science

JF - Advanced Science

SN - 2198-3844

IS - 20

M1 - 2102788

ER -

ID: 85025199