Research output: Contribution to journal › Article › peer-review
Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes : A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia. / Wu, Cheng Ham; Kisel, Kristina S.; Thangavel, Muthu Kumar; Chen, Yi Ting; Chang, Kai Hsin; Tsai, Ming Rung; Chu, Chia Yu; Shen, Yu Fang; Wu, Pei Chun; Zhang, Zhiming; Liu, Tzu Ming; Jänis, Janne; Grachova, Elena V.; Shakirova, Julia R.; Tunik, Sergey P.; Koshevoy, Igor O.; Chou, Pi Tai.
In: Advanced Science, Vol. 8, No. 20, 2102788, 20.10.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Functionalizing Collagen with Vessel-Penetrating Two-Photon Phosphorescence Probes
T2 - A New In Vivo Strategy to Map Oxygen Concentration in Tumor Microenvironment and Tissue Ischemia
AU - Wu, Cheng Ham
AU - Kisel, Kristina S.
AU - Thangavel, Muthu Kumar
AU - Chen, Yi Ting
AU - Chang, Kai Hsin
AU - Tsai, Ming Rung
AU - Chu, Chia Yu
AU - Shen, Yu Fang
AU - Wu, Pei Chun
AU - Zhang, Zhiming
AU - Liu, Tzu Ming
AU - Jänis, Janne
AU - Grachova, Elena V.
AU - Shakirova, Julia R.
AU - Tunik, Sergey P.
AU - Koshevoy, Igor O.
AU - Chou, Pi Tai
N1 - Publisher Copyright: © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH
PY - 2021/10/20
Y1 - 2021/10/20
N2 - The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI-diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.
AB - The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI-diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.
KW - phosphorescence lifetime imaging microscopy
KW - phosphorescent oxygen sensors
KW - Re diimine carbonyl complexes
KW - tissue ischemia
KW - tumor hypoxia
KW - two-photon phosphorescence
KW - DESIGN
KW - RHENIUM(I) COMPLEXES
KW - Re-I diimine carbonyl complexes
KW - MITOCHONDRIA
KW - IMAGING MICROSCOPY
KW - LUMINESCENCE
KW - ABSORPTION CROSS-SECTIONS
KW - PHOTOPHYSICS
KW - EMISSION
KW - METAL-COMPLEXES
KW - LIVING CELLS
KW - ReI diimine carbonyl complexes
UR - http://www.scopus.com/inward/record.url?scp=85113135189&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/2406aca4-9c65-3a96-8c16-8e7204fb04fa/
U2 - 10.1002/advs.202102788
DO - 10.1002/advs.202102788
M3 - Article
C2 - 34414696
AN - SCOPUS:85113135189
VL - 8
JO - Advanced Science
JF - Advanced Science
SN - 2198-3844
IS - 20
M1 - 2102788
ER -
ID: 85025199