TY - JOUR

T1 - Functional mapping of the lower urinary tract by epidural electrical stimulation of the spinal cord in decerebrated cat model

AU - Сысоев, Юрий Игоревич

AU - Баженова, Елена Юрьевна

AU - Шкорбатова, Полина Юрьевна

AU - Ковалев, Глеб Валерьевич

AU - Лабетов, Иван Антонович

AU - Меркульева, Наталья Сергеевна

AU - Шкарупа, Дмитрий Дмитриевич

AU - Мусиенко, Павел Евгеньевич

PY - 2024/4/26

Y1 - 2024/4/26

N2 - Several neurologic diseases including spinal cord injury, Parkinson's disease or multiple sclerosis are accompanied by disturbances of the lower urinary tract functions. Clinical data indicates that chronic spinal cord stimulation can improve not only motor function but also ability to store urine and control micturition. Decoding the spinal mechanisms that regulate the functioning of detrusor (Detr) and external urethral sphincter (EUS) muscles is essential for effective neuromodulation therapy in patients with disturbances of micturition. In the present work we performed a mapping of Detr and EUS activity by applying epidural electrical stimulation (EES) at different levels of the spinal cord in decerebrated cat model. The study was performed in 5 adult male cats, evoked potentials were generated by EES aiming to recruit various spinal pathways responsible for LUT and hindlimbs control. Recruitment of Detr occurred mainly with stimulation of the lower thoracic and upper lumbar spinal cord (T13-L1 spinal segments). Responses in the EUS, in general, occurred with stimulation of all the studied sites of the spinal cord, however, a pronounced specificity was noted for the lower lumbar/upper sacral sections (L7-S1 spinal segments). These features were confirmed by comparing the normalized values of the slope angles used to approximate the recruitment curve data by the linear regression method. Thus, these findings are in accordance with our previous data obtained in rats and could be used for development of novel site-specific neuromodulation therapeutic approaches.

AB - Several neurologic diseases including spinal cord injury, Parkinson's disease or multiple sclerosis are accompanied by disturbances of the lower urinary tract functions. Clinical data indicates that chronic spinal cord stimulation can improve not only motor function but also ability to store urine and control micturition. Decoding the spinal mechanisms that regulate the functioning of detrusor (Detr) and external urethral sphincter (EUS) muscles is essential for effective neuromodulation therapy in patients with disturbances of micturition. In the present work we performed a mapping of Detr and EUS activity by applying epidural electrical stimulation (EES) at different levels of the spinal cord in decerebrated cat model. The study was performed in 5 adult male cats, evoked potentials were generated by EES aiming to recruit various spinal pathways responsible for LUT and hindlimbs control. Recruitment of Detr occurred mainly with stimulation of the lower thoracic and upper lumbar spinal cord (T13-L1 spinal segments). Responses in the EUS, in general, occurred with stimulation of all the studied sites of the spinal cord, however, a pronounced specificity was noted for the lower lumbar/upper sacral sections (L7-S1 spinal segments). These features were confirmed by comparing the normalized values of the slope angles used to approximate the recruitment curve data by the linear regression method. Thus, these findings are in accordance with our previous data obtained in rats and could be used for development of novel site-specific neuromodulation therapeutic approaches.

KW - Animals

KW - Cats

KW - Decerebrate State/physiopathology

KW - Electric Stimulation/methods

KW - Epidural Space

KW - Male

KW - Spinal Cord Stimulation/methods

KW - Spinal Cord/physiopathology

KW - Urethra/physiopathology

KW - Urinary Bladder/physiopathology

KW - Urinary Tract/physiopathology

KW - Urination/physiology

UR - https://www.mendeley.com/catalogue/5b4ac249-fc88-3336-8274-c4efb8133991/

U2 - 10.1038/s41598-024-54209-3

DO - 10.1038/s41598-024-54209-3

M3 - Article

C2 - 38670988

VL - 14

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 9654

ER -