Research output: Contribution to journal › Article › peer-review
Fullerenes on a Nanodiamond Platform Demonstrate Antibacterial Activity with Low Cytotoxicity. / Bolshakova, Olga; Lebedev, Vasily; Mikhailova, Elena; Zherebyateva, Olga; Aznabaeva, Liliya; Burdakov, Vladimir; Kulvelis, Yuri; Yevlampieva, Natalia; Mironov, Andrey; Miroshnichenko, Igor; Sarantseva, Svetlana.
In: Pharmaceutics, Vol. 15, No. 7, 1984, 19.07.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Fullerenes on a Nanodiamond Platform Demonstrate Antibacterial Activity with Low Cytotoxicity
AU - Bolshakova, Olga
AU - Lebedev, Vasily
AU - Mikhailova, Elena
AU - Zherebyateva, Olga
AU - Aznabaeva, Liliya
AU - Burdakov, Vladimir
AU - Kulvelis, Yuri
AU - Yevlampieva, Natalia
AU - Mironov, Andrey
AU - Miroshnichenko, Igor
AU - Sarantseva, Svetlana
PY - 2023/7/19
Y1 - 2023/7/19
N2 - Carbon nanoparticles with antimicrobial properties, such as fullerenes, can be distinguished among the promising means of combating pathogens characterized by resistance to commercial antibiotics. However, they have a number of limitations for their use in medicine. In particular, the insolubility of carbon nanoparticles in water leads to a low biocompatibility and especially strong aggregation when transferred to liquid media. To overcome the negative factors and enhance the action of fullerenes in an extended range of applications, for example, in antimicrobial photodynamic therapy, we created new water-soluble complexes containing, in addition to C60 fullerene, purified detonation nanodiamonds (AC960) and/or polyvinylpyrrolidone (PVP). The in vitro antibacterial activity and toxicity to human cells of the three-component complex C60+AC960+PVP were analyzed in comparison with binary C60+PVP and C60+AC960. All complexes showed a low toxicity to cultured human skin fibroblasts and ECV lines, as well as significant antimicrobial activity, which depend on the type of microorganisms exposed, the chemical composition of the complex, its dosage and exposure time. Complex C60+PVP+AC960 at a concentration of 175 µg/mL showed the most stable and pronounced inhibitory microbicidal/microbiostatic effect.
AB - Carbon nanoparticles with antimicrobial properties, such as fullerenes, can be distinguished among the promising means of combating pathogens characterized by resistance to commercial antibiotics. However, they have a number of limitations for their use in medicine. In particular, the insolubility of carbon nanoparticles in water leads to a low biocompatibility and especially strong aggregation when transferred to liquid media. To overcome the negative factors and enhance the action of fullerenes in an extended range of applications, for example, in antimicrobial photodynamic therapy, we created new water-soluble complexes containing, in addition to C60 fullerene, purified detonation nanodiamonds (AC960) and/or polyvinylpyrrolidone (PVP). The in vitro antibacterial activity and toxicity to human cells of the three-component complex C60+AC960+PVP were analyzed in comparison with binary C60+PVP and C60+AC960. All complexes showed a low toxicity to cultured human skin fibroblasts and ECV lines, as well as significant antimicrobial activity, which depend on the type of microorganisms exposed, the chemical composition of the complex, its dosage and exposure time. Complex C60+PVP+AC960 at a concentration of 175 µg/mL showed the most stable and pronounced inhibitory microbicidal/microbiostatic effect.
KW - antibacterial activity
KW - biofilms
KW - cell
KW - fullerene
KW - microorganism
KW - nanodiamond
KW - polymer
KW - toxicity
UR - https://www.mendeley.com/catalogue/cabc3459-b08d-304d-be91-e9093c42c6cc/
U2 - DOI: 10.3390/pharmaceutics15071984
DO - DOI: 10.3390/pharmaceutics15071984
M3 - Article
C2 - 37514170
VL - 15
JO - Pharmaceutics
JF - Pharmaceutics
SN - 1999-4923
IS - 7
M1 - 1984
ER -
ID: 115012837