Research output: Contribution to journal › Article › peer-review
Formation of Soluble Complexes of Cationic Polypyridylphenylene Dendrimers with DNA. / Kuchkina, N. V.; Laptinskaya, T. V.; Tsvetkov, N. V.; Rusanov, A. L.; Izumrudov, V. A.; Shifrina, Z. B.
In: Polymer Science - Series C, Vol. 52, No. 1, 2010, p. 105-110.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Formation of Soluble Complexes of Cationic Polypyridylphenylene Dendrimers with DNA
AU - Kuchkina, N. V.
AU - Laptinskaya, T. V.
AU - Tsvetkov, N. V.
AU - Rusanov, A. L.
AU - Izumrudov, V. A.
AU - Shifrina, Z. B.
PY - 2010
Y1 - 2010
N2 - The complexation of DNA with a dendrimer with the successive addition of the dendrimer solution to the solution of DNA leads to the appearance and accumulation of compact particles with a diameter of Dh=100 10 nm that coexist with particles whose dimensions coincide with those of free DNA molecules (Dh = 450 50 nm). The fraction of large particles decreases regularly; moreover, when the equimolar ratio of charged groups of interacting components is achieved, these particles disappear. The coexistence of practically free DNA molecules and a compact soluble negatively charged complex is confirmed by a sedimentation assay. Further addition of the dendrimer up to a certain ratio of charged groups of the components, much higher than unity, brings about phase separation, while at a large excess of charge groups of the dendrimer (above fivefold), the system turns out to be fully single-phase and composed of compact (110 10 nm) cationic soluble complexes. Owing to the positive charge and small size of complex part
AB - The complexation of DNA with a dendrimer with the successive addition of the dendrimer solution to the solution of DNA leads to the appearance and accumulation of compact particles with a diameter of Dh=100 10 nm that coexist with particles whose dimensions coincide with those of free DNA molecules (Dh = 450 50 nm). The fraction of large particles decreases regularly; moreover, when the equimolar ratio of charged groups of interacting components is achieved, these particles disappear. The coexistence of practically free DNA molecules and a compact soluble negatively charged complex is confirmed by a sedimentation assay. Further addition of the dendrimer up to a certain ratio of charged groups of the components, much higher than unity, brings about phase separation, while at a large excess of charge groups of the dendrimer (above fivefold), the system turns out to be fully single-phase and composed of compact (110 10 nm) cationic soluble complexes. Owing to the positive charge and small size of complex part
U2 - 10.1134/S1811238210010121
DO - 10.1134/S1811238210010121
M3 - Article
VL - 52
SP - 105
EP - 110
JO - Polymer Science - Series C
JF - Polymer Science - Series C
SN - 1811-2382
IS - 1
ER -
ID: 5205813