[Formation of antibodies against neuraminidase of A/California/07/2009 (H1N1) influenza virus after immunization with live monovalent influenza vaccine]. / Smolonogina, T. A.; Desheva, I. A.; Shaldzhian, A. A.; Grudinin, M. P.; Rudenko, L. G.
In: Zhurnal mikrobiologii, epidemiologii, i immunobiologii, No. 6, 01.11.2011, p. 72-76.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - [Formation of antibodies against neuraminidase of A/California/07/2009 (H1N1) influenza virus after immunization with live monovalent influenza vaccine].
AU - Smolonogina, T. A.
AU - Desheva, I. A.
AU - Shaldzhian, A. A.
AU - Grudinin, M. P.
AU - Rudenko, L. G.
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Detection of antibodies against neuraminidase (NA) of A/California/07/2009 (H1N1) influenza virus in blood sera of volunteers after the immunization with live monovalent influenza vaccine (LIV). Neuraminidase enzyme activity inhibition by antibodies test with reassortant strain A(H7N1) containing NA of pandemic strain was used. Anti-neuraminidase IgG antibodies against whole reassortant virus A(H7N1) and purified NA of A/California/07/2009 (H1N1) strains were determined by enzyme immunoassay (EIA). After two immunizations with LIV of seronegative individuals a 1.5 times mean increase of antibodies against homologous neuraminidase was detected (by hemagglutinin inhibition reaction). The high level of anti-neuraminidase antibodies were detected in individuals that had been naturally infected. A correlation between anti-neuraminidase IgG antibody titers obtained in EIA with whole reassortant virus A(H7N1) and purified protein was demonstrated. Modified sialidase activity inhibition method and EIA with reassortant diagnostic strain can be applied to evaluate anti-neuraminidase antibodies.
AB - Detection of antibodies against neuraminidase (NA) of A/California/07/2009 (H1N1) influenza virus in blood sera of volunteers after the immunization with live monovalent influenza vaccine (LIV). Neuraminidase enzyme activity inhibition by antibodies test with reassortant strain A(H7N1) containing NA of pandemic strain was used. Anti-neuraminidase IgG antibodies against whole reassortant virus A(H7N1) and purified NA of A/California/07/2009 (H1N1) strains were determined by enzyme immunoassay (EIA). After two immunizations with LIV of seronegative individuals a 1.5 times mean increase of antibodies against homologous neuraminidase was detected (by hemagglutinin inhibition reaction). The high level of anti-neuraminidase antibodies were detected in individuals that had been naturally infected. A correlation between anti-neuraminidase IgG antibody titers obtained in EIA with whole reassortant virus A(H7N1) and purified protein was demonstrated. Modified sialidase activity inhibition method and EIA with reassortant diagnostic strain can be applied to evaluate anti-neuraminidase antibodies.
UR - http://www.scopus.com/inward/record.url?scp=84873054000&partnerID=8YFLogxK
M3 - статья
C2 - 22308734
AN - SCOPUS:84873054000
SP - 72
EP - 76
JO - Журнал микробиологии, эпидемиологии и иммунобиологии
JF - Журнал микробиологии, эпидемиологии и иммунобиологии
SN - 0372-9311
IS - 6
ER -
ID: 45925728